2013
DOI: 10.3389/fpls.2013.00011
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Defining the Core Proteome of the Chloroplast Envelope Membranes

Abstract: High-throughput protein localization studies require multiple strategies. Mass spectrometric analysis of defined cellular fractions is one of the complementary approaches to a diverse array of cell biological methods. In recent years, the protein content of different cellular (sub-)compartments was approached. Despite of all the efforts made, the analysis of membrane fractions remains difficult, in that the dissection of the proteomes of the envelope membranes of chloroplasts or mitochondria is often not relia… Show more

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Cited by 65 publications
(69 citation statements)
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References 93 publications
(154 reference statements)
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“…Although the plasma membrane marker H + -ATPase was also found in those preparations, a genuine chloroplast location for one or several isoforms of the PIP family was proposed. Most recently, TIP1;1 and PIP2;1 (also named PIP2A) were identified by mass spectrometry in the Arabidopsis envelope fraction [32].…”
Section: Introductionmentioning
confidence: 99%
“…Although the plasma membrane marker H + -ATPase was also found in those preparations, a genuine chloroplast location for one or several isoforms of the PIP family was proposed. Most recently, TIP1;1 and PIP2;1 (also named PIP2A) were identified by mass spectrometry in the Arabidopsis envelope fraction [32].…”
Section: Introductionmentioning
confidence: 99%
“…Such autoubiquitination may lead to proteasomal degradation, as SP1 protein accumulated following proteasome inhibitor treatment [112]. This may explain why proteomic analyses of the chloroplast envelope have not uncovered SP1 [138,139]. Autoubiquitination is also likely to occur at SGR9, as it was shown that SGR9 protein can be stabilized in vivo by mutation of its RING domain causing loss of E3 ligase activity [132].…”
Section: The Ups and Chloroplast Resident Proteinsmentioning
confidence: 90%
“…Interestingly, a recent proteomic study on purified chloroplast envelopes identified ubiquitin and some ubiquitin ligases [139]. However, contamination cannot be ruled out because of their high abundance.…”
Section: The Ups and Chloroplast Resident Proteinsmentioning
confidence: 98%
“…Chloroplast outer and inner envelopes, as well as thylakoids, can be efficiently separated by centrifugation techniques, and their respective proteomes have been extensively characterized [28,[108][109][110]. These data revealed that lipid and carotenoid synthesis is localized to the envelope membranes.…”
Section: Lessons From Membrane Fractionation Experiments -Thylakoid Hmentioning
confidence: 96%