Defining the Bacillus subtilis σW regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches

Cao, Min; Kobel, Phil; Morshedi, Maud M.; Wu, Ming; Paddon, Christopher J.; Helmann, John D.

doi:10.1006/jmbi.2001.5372

Cited by 179 publications

(212 citation statements)

References 35 publications

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“…Exceptions include the fosB fosfomycin-resistance gene (Cao et al, 2001), the pbpE penicillin-binding protein (Popham and Setlow, 1993), and sppA which may play a role in degradation of cleaved signal peptides (Bolhuis et al, 1999). Bioinformatic analysis suggests that several other σ W -dependent operons may function in bacteriocin or antibiotic resistance (Cao et al, 2002b). By studying B. subtilis in competition with other Bacilli, we have provided evidence that the σ W regulon plays an important role in providing intrinsic resistance against antibiotics produced by competing strains.…”

Section: Discussionmentioning

confidence: 97%

“…The σ W regulon includes about 60 genes, almost all of unknown function (Huang et al, 1998;Cao et al, 2002b). Exceptions include the fosB fosfomycin-resistance gene (Cao et al, 2001), the pbpE penicillin-binding protein (Popham and Setlow, 1993), and sppA which may play a role in degradation of cleaved signal peptides (Bolhuis et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…To determine which genes in the σ W regulon confer resistance against B. amyloliquefaciens FZB42, we surveyed a comprehensive panel of strains individually disrupted for each of 30 σ W -dependent operons (Cao et al, 2002b; Table S1). Only deletions of ydbST or fosB resulted in detectable increases in sensitivity to B. amyloliquefaciens.…”

Section: W Provides Resistance To Compounds Produced By Other Bacillumentioning

confidence: 99%

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Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Butcher

Helmann²

2006

Molecular Microbiology

177

184

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SummaryBacillus subtilis produces many antibiotics of varying structures and specificity. Here we identify a prominent role for s W , an extracytoplasmic function (ECF) sigma factor, in providing intrinsic resistance to antimicrobial compounds produced by other Bacilli . By using a panel of B. subtilis mutants disrupted for each of the 30 known s W -dependent operons we identified resistance genes for at least three different antimicrobial compounds. The ydbST and fosB genes contribute to resistance to antimicrobial compound(s) produced by B. amyloliquefaciens FZB42, the yqeZyqfAB operon provides resistance to the SP b prophage-encoded bacteriocin sublancin, and the yknWXYZ operon and yfhL provide resistance to the antimicrobial peptide SdpC. YfhL encodes a paralogue of SdpI, a membrane protein that provides immunity to SdpC. In competition experiments, we identify s W as a key factor in allowing B. subtilis to resist antibiotic killing and encroachment by competing strains. Together with the previous observation that s W provides inducible resistance against the Streptomyces antibiotic fosfomycin, these studies support the notion that s W controls an antibiosis regulon important in the microbial ecology of soil bacteria.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: W Provides Resistance To Compounds Produced By Other Bacillumentioning

confidence: 99%

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Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Butcher

Helmann²

2006

Molecular Microbiology

177

184

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“…4). 34) BHSigW and GKSigW, expressed in B. subtilis, were active and were concerned with transcription (Table 3). Hence, if the specificities for promoter recognition of all SigWs are similar, it is peculiar that only OISigW caused a toxic phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Whole DNA sequences encompassing the OIsigW and OIrsiW genes integrated Underlined sequences indicate À35, À10, and þ1 regions of promoter recognized by BSSigW. 34) Dashed-underlined sequences indicate putative SigW-dependent promoters based on the consensus sequence described above.…”

Section: Revertant Of the Growth-defective Phenotype Of The Oirsiw-inmentioning

confidence: 99%

Heterologous Expression of theOceanobacillus iheyensisSigW and Its Anti-Protein RsiW inBacillus subtilis

Yano

Inoue

Mori

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Pairs of the ECF sigma factor and its anti-sigma factor, SigW and RsiW, of Bacillus-related species that inhabit extreme environments were heterologously expressed in B. subtilis. All the RsiWs, membrane proteins, failed to fill their function of repressing cognate SigW activity, despite their close structural similarities. Particularly, uncontrolled expression of Oceanobacillus iheyensis OISigW due to abortive OIRsiW was harmful to B. subtilis. Analysis of revertants of this growth defect and site-directed mutagenesis indicated that the insertion of six and a minimum of three hydrophobic amino acid residues occurring in the transmembrane region allowed OIRsiW to function as anti-OISigW. Subcellular localization of OIRsiW was detected by immunoblot analysis, suggesting that both the wild-type and the mutant form of OIRsiW were localized to the membrane. An appropriate length of a transmembrane region required for proper integration into the membrane after translocation might vary among these Bacillus-related species.

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Bacterial Stress Responses as Determinants of Antimicrobial Resistance

and

Poole

2016

Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria

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Defining the Bacillus subtilis σW regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches

Cited by 179 publications

References 35 publications

Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Heterologous Expression of theOceanobacillus iheyensisSigW and Its Anti-Protein RsiW inBacillus subtilis

Bacterial Stress Responses as Determinants of Antimicrobial Resistance

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Defining the Bacillus subtilis σW regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches

Cited by 179 publications

References 35 publications

Identification of Bacillus subtilis σW‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Identification of Bacillus subtilis σW‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Heterologous Expression of theOceanobacillus iheyensisSigW and Its Anti-Protein RsiW inBacillus subtilis

Bacterial Stress Responses as Determinants of Antimicrobial Resistance

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Product

Resources

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Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli

Identification of Bacillus subtilis σ^W‐dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli