Defining the Anti-Metamorphic Action of Juvenile Hormone

Wilis, J. H.; Cox, Diana L.

doi:10.1007/978-3-642-69922-1_45

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…This conclusion has now been confirmed by immunogold labeling of ultrathin frozen sections (Wolfgang, W. J., and D. Fristrom, manuscript in preparation). Differential synthesis of cuticle proteins can also be tissue related (55). In the results presented here it is clear that PCP15 is confined to the anterior integument (see also reference 5).…”

Section: Discussionsupporting

confidence: 53%

The pupal cuticle of Drosophila: biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone.

Doctor¹,

Fristrom²,

Fristrom³

1985

The Journal of Cell Biology

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We investigated the synthesis and localization of Drosophila pupal cuticle proteins by immunochemical techniques using both a complex antiserum and monoclonal antibodies. A set of low molecular weight (15,000-25,000) pupal cuticle proteins are synthesized by the imaginal disk epithelium before pupation. After pupation, synthesis of the low molecular weight proteins ceases and a set of unrelated high molecular weight proteins (40,000-82,000) are synthesized and incorporated into the pupal cuticle. Ultrastructural changes in the cuticle deposited before and after pupation correlate with the switch in cuticle protein synthesis. A similar biphasic accumulation of low and high molecular weight pupal cuticle proteins is also seen in imaginal discs cultured in vitro. The low molecular weight pupal cuticle proteins accumulate in response to a pulse of the insect steroid hormone 20-hydroxyecdysone and begin to appear 6 h after the withdrawal of the hormone from the culture medium. The high molecular weight pupal cuticle proteins accumulate later in culture; a second pulse of hormone appears to be necessary for the accumulation of two of these proteins.

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Section: Discussionsupporting

confidence: 53%

The pupal cuticle of Drosophila: biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone.

Doctor¹,

Fristrom²,

Fristrom³

1985

The Journal of Cell Biology

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“…Application of JH to pupae of the dipteran, S. bullata causes the secretion of a second cuticle with pupal characteristics (Srivastava and Gilbert, 1968;. Thus, in common with Lepidoptera and other insects, the change from pupal to adult differentiation requires the absence of JH (Willis, 1981;Willis and Cox, 1984).…”

Section: Prepupae/pupaementioning

confidence: 99%

Characterisation of juvenile hormone esterase in Drosophila melanogaster

Campbell

Healy

Oakeshott

1992

Insect Biochemistry and Molecular Biology

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Juvenile hormone (JH) is one of the main morphogenic hormones of insects and degradation of the hormone is believed to play an important role in its regulation in many insects. The two main routes for JH degradation are hydroy sis by JH esterase (JHE) and JH epoxide hydrolase (JHEH). • A major focus of research has been on juvenile hormone esterase (JHE) found in the haemolymph of final instar lepidopteran larvae, although considerable hydrolysis of JH occurs in other tissues and at other life stages. In contrast, no comprehensive study of JH degradation has been undertaken in any dipteran species. However, the available evidence suggests that very little JH degrading activity is found in the haemolymph of Diptera and several dipteran JHEs exhibit atypical patterns of sensitivity to inhibitors. These data suggested that both the expression pattern and the biochemical properties of dipteran JH degrading enzymes may differ from other insect orders, and therefore, that the role of JH degradation may be different.The aim of this project was to comprehensively characterise JH degradation in the dipteran, Drosophila melanogaster, with special emphasis on JHE. The first objective was to identify the JH degrading enzymes of D . melanogaster and the second was to deterrnine their expression patterns and biochemical properties in order to determine thei~ role in the regulation of JH titre. These data were compared with other data to deterrnine whether a general model could be developed for dipteran JH titre regulation. Finally, the roles of JH hydrolysis in the regulation of JH titre were compared among Diptera, Lepidoptera and other insects.Two approaches were taken to identify the JH degrading enzymes of D. melanogaster. The first was to investigate the properties of an esterase, denoted EST20, which can be visualised after native polyacrylarnide gel electrophoresis using artificial esterase substrates, a-and B-naphthylacetate. EST20 is shown to be a likely homologue of the p-esterase of Drosophila virilis, which has been claimed to be the JHE of this species. However, this study shows that JH hydrolytic activity is associated with neither EST20 in D. melanogaster nor the p-esterase in D . virilis.The second approach was to use a radiometric assay which identified two JH hydrolysing enzymes, JHE and JHEH. The developmental profile of JH hydrolytic activity in D. melanogaster correlates inversely with profiles of JH titre and production. Key features are prewandering and prepupal maxima of JH hydrolytic activity in whole body homogenates of final instar larvae. This developmental profile of expression is similar to the lepidopteran pattern suggesting that the role of JH hydrolysis is essentially similar during this period. In D. melanogaster, the prewandering maximum consists mainly of JHEH whereas the prepupal peak consists mainly of JHE. However, the relative contributions of JHE and JHEH at these times differs both among the Diptera and in other orders.JHE from the prepupal stage was purified to homogeneity by selectiv...

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“…It is possible to get hybrids between two species of Hyalophora, H. cecropia and H. gloveri. We found that HCCP12 had different electrophoretic mobility in the two species, and both bands were present in both larvae and adults of the hybrids (55). Hence, the same gene was used in both metamorphic stages.…”

Section: Looking At Gelsmentioning

confidence: 85%

“…How can adjacent epidermal cells respond with different phenotypes while basking in the same hormonal milieu at each life stage? Possibly, as I and others mentioned years ago, it may have something to do with chromatin structure guiding what genes are accessible to transcription factors (55). ]…”

Section: Wwwannualreviewsorg • Arthropod Proteomics and Genomicsmentioning

confidence: 99%

The Evolution and Metamorphosis of Arthropod Proteomics and Genomics

Willis

2018

Annu. Rev. Entomol.

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This article presents an overview of the development of techniques for analyzing cuticular proteins (CPs), their transcripts, and their genes over the past 50 years based primarily on experience in the laboratory of J.H. Willis. It emphasizes changes in the kind of data that can be gathered and how such data provided insights into the molecular underpinnings of insect metamorphosis and cuticle structure. It describes the techniques that allowed visualization of the location of CPs at both the anatomical and intracuticular levels and measurement of the appearance and deployment of transcripts from CP genes as well as what was learned from genomic and transcriptomic data. Most of the early work was done with the cecropia silkmoth, Hyalophora cecropia, and later work was with Anopheles gambiae.

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Defining the Anti-Metamorphic Action of Juvenile Hormone

Cited by 8 publications

References 12 publications

The pupal cuticle of Drosophila: biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone.

The pupal cuticle of Drosophila: biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone.

Characterisation of juvenile hormone esterase in Drosophila melanogaster

The Evolution and Metamorphosis of Arthropod Proteomics and Genomics

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