Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells

Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions

“…This is a novel approach and the influence of MMC on ASC characteristics has not been previously reported. However, we and others have shown that different culture conditions greatly influence ASC proliferation and differentiation capacity [24, 29, 30, 39]. As cell-based treatments are becoming more common, efficient in vitro cell expansion and differentiation methods are important to achieve an optimal and predictable clinical outcome.…”

“…Also, antibodies against bovine antigens may be elicited by repeated administration of cells, which will affect the efficacy of cell-based treatments. Therefore, xeno-free (XF) alternatives such as human serum- (HS-) [23–25] or platelet- (PL-) derived supplements [26–28] as well as fully defined XF and serum-free (SF) conditions [29, 30] have been developed for ASC culture. HS-based approaches have not only potential for clinical use but also disadvantages, for example, limited availability and lot-to-lot variation [31].…”

“…HS-based approaches have not only potential for clinical use but also disadvantages, for example, limited availability and lot-to-lot variation [31]. These challenges can be avoided by replacing undefined and/or animal-derived components with defined XF/SF reagents that have a reproducible composition [29, 30]. Moreover, careful characterization of ASC behavior in clinically relevant in vitro culture conditions is important for the progress of cell-based therapies.…”

The effect of medium selection on adipose-derived stem cell expansion and differentiation: implications for application in regenerative medicine

“…The use of FBS as a supplement for growing cultured cells raises regulatory issues for clinical application, thus media which do not need FBS should be further investigated. Others have investigated serum-and xenobiotic-free medium systems or those which substitute FBS with human serum (Shih et al Chase et al 2012;Yang et al 2012;Patrikoski et al 2013;Al Saqi et al 2014). For example, Patrikoski et al (2013) have reported the culture of ADSCs in xeno-and serum-free media as well as in medium supplemented with human serum.…”

“…Their findings included a reduction in adhesion of the cells to tissue culture plastic in xeno-and serum-free media without applying a carboxyl coating but cell proliferation in further passages was good and population doubling faster than in media containing human serum. More recently, Al- Saqi et al (2014) have compared ADSC culture in a commercially available xenobiotic-free medium formulation to culture in DMEM with 10 % FBS and found the former to support more rapid cell proliferation and differentiation. Further work on establishing robust xenobiotic-free medium formulations in the development of protocols for clinical application for human patients should include comparison of several such formulations to produce sufficient data to obtain health authority approval for use of such systems.…”