Defined Populations of Bone Marrow Derived Mesenchymal Stem and Endothelial Progenitor Cells for Bladder Regeneration

Abstract

Data demonstrate that mesenchymal stem cells and smooth muscle cells express the same contractile proteins and can function similarly in vitro. Endothelial progenitor cells also have the ability to form vasculature in an in vivo chorioallantoic membrane model. These findings provide evidence that mesenchymal stem cells and endothelial progenitor cells have characteristics that may be applicable for bladder tissue regeneration.

A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

“…The characterization of a battery of contractile proteins shows that MSCs have a contractile protein profile similar to bSMCs [8]. Further data presented in this study demonstrate both cell types can also respond to agonist stimulation with statistically indistinguishable contractile responses in vitro.…”

“…Experimental data has demonstrated the utility of bone marrow (BM) cells in a bladder regenerative setting with data suggesting the phenotypic and physiological similarities between mesenchymal stem cells (MSCs) and bSMCs [7][8][9]. The characterization of a battery of contractile proteins shows that MSCs have a contractile protein profile similar to bSMCs [8].…”

Tissue Engineering for Tissue and Organ Regeneration

“…(Quirici, 2001) Sharma et al describe the in vivo potential of this cell population as it was seeded within a chicken chorioallantoic membrane model. (Sharma, 2009) Post experimentation analyses of this work clearly demonstrate the angiogenic potential of bone marrow derived CD34 + /CD133 + cells as human specific vasculature was formed utilizing this model. Other derivatives of this cell population including CD34 + /CD133 + /VEGFR2 + , CD34 + /CD133 + /VEGFR3 + , CD34 + /VEGFR2 + , and CD133 + /VEGFR2 + cells maintain similar properties.…”

“…(Sharma, 2009) The mesenchymal stem cells utilized throughout this study as well as subsequent studies were an epitope defined population of highly purified mesenchymal stem cells. This study further demonstrated that both populations of cells can respond to agonist stimulation with statistically indistinguishable contractile responses in vitro while possessing similar contractile machinery.…”

Mesenchymal Stem Cells for Cell Therapy and Tissue Regeneration in Urology

“…Recently, BMSC were evaluated as an alternative cell type for use in replacement of bladder SMC when native bladder muscle tissue is unavailable. The potential of BMSC to differentiate into cells with bladder SMC characteristics was assessed in vitro 72 and in different animal models [73][74][75][76][77][78] . Kanematsu et al 75 showed that in vitro, both supernatants from cultured rat bladder cells (conditioned media) or media containing TGFand VEGF induced bone marrow cells to adopt a SMC phenotype.…”