Deficient Proliferation and Apoptosis in the Granulosa and Theca Interna Cells of the Bovine Cystic Follicle

Isobe, Naoki; Yoshimura, Y.

doi:10.1262/jrd.19041

Cited by 51 publications

(51 citation statements)

References 27 publications

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“…Previous studies found that the mRNA levels for cyclins D1 and E in samples of the follicular wall are lower in cystic follicles than in healthy tertiary follicles (Isobe & Yoshimura 2007. Similarly to the results found in induced follicular cysts in rats in different experimental models (Table 1; Baravalle et al 2006, tertiary follicles of cows show an intense proliferation in the basal area of the granulosa layer, whereas in atretic and cystic follicles proliferation declines (Isobe & Yoshimura 2007.…”

Section: R258 H H Ortega and Otherssupporting

confidence: 56%

Molecular aspects of bovine cystic ovarian disease pathogenesis

Ortega¹,

Marelli²,

Rey³

et al. 2015

Reproduction

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Cystic ovarian disease (COD) is one of the main causes of reproductive failure in cattle and causes severe economic loss to the dairy farm industry because it increases both days open in the post partum period and replacement rates due to infertility. This disease is the consequence of the failure of a mature follicle to ovulate at the time of ovulation in the estrous cycle. This review examines the evidence for the role of altered steroid and gonadotropin signaling systems and the proliferation/apoptosis balance in the ovary with cystic structures. This evidence suggests that changes in the expression of ovarian molecular components associated with these cellular mechanisms could play a fundamental role in the pathogenesis of COD. The evidence also shows that gonadotropin receptor expression in bovine cystic follicles is altered, which suggests that changes in the signaling system of gonadotropins could play a fundamental role in the pathogenesis of conditions characterized by altered ovulation, such as COD. Ovaries from animals with COD exhibit a disrupted steroid receptor pattern with modifications in the expression of coregulatory proteins. These changes in the pathways of endocrine action would trigger the changes in proliferation and apoptosis underlying the aberrant persistence of follicular cysts. Free Spanish abstract: A Spanish translation of this abstract is freely available at

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Section: R258 H H Ortega and Otherssupporting

confidence: 56%

Molecular aspects of bovine cystic ovarian disease pathogenesis

Ortega¹,

Marelli²,

Rey³

et al. 2015

Reproduction

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show abstract

“…Many studies have demonstrated changes and pathogenetic mechanisms evolved in follicular cyst in cattle (Garverick 1997, Isobe & Yoshimura 2007, Ortega et al 2007b, Monniaux et al 2008, Ortega et al 2008, Rey et al 2010), rat (Anderson & Lee 1997, Ortega et al 2007a, Salvetti et al 2009) and women (Abbot et al 2002, Ortega et al 2009b), but few investigations have been conducted in the genesis of this condition in sows. The current study shows that the formation of ovarian cysts in sows is associated to alterations in the expression of some cytoskeletal proteins.…”

Section: Discussionmentioning

confidence: 99%

Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

et al. 2015

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The expression of cytoskeletal proteins was evaluated immunohistochemically in 36 normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, was evaluated. All sows had history of reproductive problems, such as infertility or subfertility. The immunohistochemically stained area (IHCSA) was quantified through image analysis to evaluate the expression of these proteins in the follicular wall of secondary, tertiary, and cystic follicles. Cytokeratins (CK) immunoreactivity was strong in the granulosa cell layer (GC) and mild in the theca interna (TI) and externa (TE) of the normal follicles. There was severe reduction of the reaction to CK in the GC in the cystic follicles, mainly in the luteinized cysts. The immunoreactivity for vimentin was higher in the GC from normal and cystic follicles in contrast with the other follicular structures. In the luteinized cysts, the IHCSA for vimentin was significantly higher in TI and in both observed cysts, the labeling was more accentuated in TE. Immunohistochemical detection of desmin and α-SMA was restricted to the TE, without differences between the normal and cystic follicles. The results of the current study show that the development of ovarian cysts in sows is associated to changes in the expression of the cytoskeletal proteins CK and vimentin.

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“…However, these concentrations change dramatically in the absence of granulosa cells [11,12]. Since E2 is produced by granulosa cells, elimination of these cells reduces the E2 concentration; E2 concentration therefore represents a check point for resolution of the stage of the cystic follicles [9,12]. The FCOs used in this study showed granulosa cell layers, high concentrations of E2 in their follicular fluid, and no significant difference from normal ovaries with respect to apoptotic cells indicated by TUNEL staining.…”

Section: Bovine Follicular Cystic Ovarymentioning

confidence: 57%

“…Follicular cysts were diagnosed based on macroscopic and endocrinological characteristics [8]. The follicular walls of the cysts were thin and translucent [9]. We observed healthy follicles (3 to 5 mm and 8 to 10 mm) in ovaries without follicles greater than 25 mm in diameter.…”

Section: Samplesmentioning

confidence: 92%

“…Apoptotic cells are more frequently seen in FCOs lacking a granulosa cell layer. The granulosa and theca interna cells of cystic follicles show weak proliferative activity and a low frequency of apoptosis, implying that the cystic follicle grows slowly and delays follicular regression [9]. Cystic follicles containing granulosa cells are distinguished by a high concentration of E2 and a low concentration of progesterone in their follicular fluids.…”

Section: Bovine Follicular Cystic Ovarymentioning

confidence: 99%

See 1 more Smart Citation

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

Choe

Cho

Kim

et al. 2010

Korean J Physiol Pharmacol

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Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and ≥25 mm). These follicles had granulosa cell layer and theca interna and the hormone 17β-estradiol (E2)/ progesterone (P4) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using GeneFishing TM DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

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Deficient Proliferation and Apoptosis in the Granulosa and Theca Interna Cells of the Bovine Cystic Follicle

Cited by 51 publications

References 27 publications

Molecular aspects of bovine cystic ovarian disease pathogenesis

Molecular aspects of bovine cystic ovarian disease pathogenesis

Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

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