Deficiency of fibroblast growth factor 2 (FGF‐2) leads to abnormal spermatogenesis and altered sperm physiology

Saucedo, Lucía; Rumpel, Regina; Sobarzo, Cristian Marcelo; Schreiner, Dietmar; Brandes, Gudrun; Lustig, Livia; Vazquez‐Levin, Mónica H.; Grothe, Claudia; Marı́n-Briggiler, Clara I.

doi:10.1002/jcp.26876

Cited by 8 publications

(6 citation statements)

References 55 publications

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“…Fibroblast growth factor 2 ( FGF2 ) plays essential functions in regulation of spermatogenesis and sperm physiology [ 33 ]. A study using a human model reported presence of FGF2 and FGFR s in testis and sperm, which are related with human spermatogenesis and sperm motility [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

et al. 2021

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Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR. Results Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed transcripts (DETs), including 20,155 in F3nRR vs. FRCC and 4,107 in M3nRR vs. M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs. FRCC and M3nRR vs. M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, respectively, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR. Conclusions Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. RNA-Seq data revealed 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish.

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Section: Discussionmentioning

confidence: 99%

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

et al. 2021

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“…Fibroblast growth factor 2 (FGF2) plays essential functions in regulation of spermatogenesis and sperm physiology [33]. A study using a human model reported presence of FGF2 and FGFRs in testis and sperm, which are related with human spermatogenesis and sperm motility [34].…”

Section: Discussionmentioning

confidence: 99%

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Wang

Luo

Qin

et al. 2021

Preprint

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Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR. Results Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed genes (DEGs), including 20,155 in F4nRR vs FRCC and 4,107 in M3nRR vs M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs FRCC and M3nRR vs M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR. Conclusions Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. Further, RNA-Seq data revealed 8 hub genes related to fertility in 3nRR. We further identified 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish.

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“…Treatment doses were formulated based on recombinant FGF2 treatments of human and mouse semen. 14,15 Reconstituted FGF2 was stored under sterile conditions between -20 to -70°C and used within 3 months (expiration date).…”

Section: Recombinant Fibroblast Growth Factormentioning

confidence: 99%

“…Furthermore, platelet lysates can be stored at -20°C up to 5 months and still maintain growth factor content stability. 11 Fibroblast growth factor (FGF2), one of the key growth factors in PRP, 12 improved characteristics of human and mouse sperm [13][14][15] and increased percent motile sperm with higher kinematics after incubation with recombinant human FGF2. 14,15 The rhFGF2 concentration (0.1-1 ng/ml) used was similar to reported doses used with human PRP (0.15 -0.30 ng/ml).…”

Section: Introductionmentioning

confidence: 99%

“…11 Fibroblast growth factor (FGF2), one of the key growth factors in PRP, 12 improved characteristics of human and mouse sperm [13][14][15] and increased percent motile sperm with higher kinematics after incubation with recombinant human FGF2. 14,15 The rhFGF2 concentration (0.1-1 ng/ml) used was similar to reported doses used with human PRP (0.15 -0.30 ng/ml). 12 The concentration of FGF2 in human ejaculates was positively correlated with total sperm number and motility.…”

Section: Introductionmentioning

confidence: 99%

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Effect of platelet rich plasma lysate and fibroblast growth factor 2 on stallion sperm motility

Pinaffi¹,

Wilborn²,

Boone³

et al. 2021

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Growth factors (GFs) are known to modulate cell function and their presence in semen could be advantageous to sperm. In humans andmice, fibroblast growth factor 2 (FGF2) improved semen motility. Platelet rich plasma (PRP), rich in GFs including FGF2, reduced postmating inflammatory response within the uterus; however, its effects on sperm are not known. PRP lysate (PRPL) is much purer than PRPand contains higher concentrations of GFs. Hence, the objective of the present study was to evaluate the effect of supplementing freshand cool-stored stallion sperm with either PRPL at 1, 2.5, 5, and 10% (also containing 1 IU/ml of heparin), or FGF2 at 0.1, 1, 10, and100 ng/ml. Motility parameters were evaluated using computer assisted semen analysis at 0, 0.5, 1, 1.5, 6, and 24 hours after treatment.For both PRPL and FGF2 treatments, there were no differences in total and progressive motility among groups. Concentrations of PRPL> 5% induced sperm agglutination via head-to-head attachment, starting at hour 1 and was more pronounced for 10% PRPL than 5%PRPL, suggesting a dose-dependent characteristic. Direct addition of PRPL to semen extender at doses < 5% might not substantiallyaffect sperm motility, whereas doses > 5% might affect sperm motility due to head-to-head attachments. Addition of FGF2 at theconcentrations studied may not affect sperm characteristics.

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Deficiency of fibroblast growth factor 2 (FGF‐2) leads to abnormal spermatogenesis and altered sperm physiology

Cited by 8 publications

References 55 publications

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Effect of platelet rich plasma lysate and fibroblast growth factor 2 on stallion sperm motility

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