Deficiency in DNA repair in mouse lymphoma strain L5178Y-S.

Evans, Helen H.; Ricanati, Marlene; Horng, Min-Fen

doi:10.1073/pnas.84.21.7562

Cited by 73 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed comparison of their properties and analysis of the differences related to their responses to ionizing radiation have been carried out. The central problem has been that of DNA damage and repair, consecutively studied with the methods of alkaline sedimentation (Korner et al 1977), alkaline unwinding (Johanson et al 1982), alkaline and neutral filter elution Hittelman 1987, 1988 a, b;Evans et al 1987), and fluorescent halos (Kapiszewska et al 1989). With the exception of alkaline sedimentation, no analyses carried out at denaturing conditions have revealed differences either in the initial DNA damage or in its repair.…”

Section: Discussionmentioning

confidence: 98%

“…The earlier finding of slower repair of DNA strand breaks [alkaline sedimentation (Korner et al 1977)] was treated with some reservation because of the high (100 Gy) radiation dose applied. When DNA damage was analyzed at denaturing pH [alkaline unwinding (Johanson et al 1982); alkaline elution (Wlodek an Hittelman 1987;Evans et al 1987)], no difference between LY-R and LY-S cells was found in either the initial damage or its repair.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells. II. Repair

Kapiszewska

Szumiel

Lange

1994

Radiat Environ Biophys

View full text Add to dashboard Cite

In the preceding paper we described the properties of nucleoids analyzed with the fluorescent halo assay at pH 6.9 and 9, as well as in the presence of reducing and chelating agents and after X-irradiation. We found analogies between the properties of type I and II nucleoids, as examined by Lebkowski and Laemmli (1982), and nucleoids analyzed with the fluorescent halo assay. We concluded that radiation-inflicted damage at two levels of DNA folding is measured at pH 6.9 and 9. In this paper we examined repair of damage to the nucleoid structure as assayed by the fluorescent halo method in X-irradiated L5178Y (LY) sublines; R (radiation resistant, D0 = 1.4 Gy) and S (radiation sensitive, D0 = 0.5 Gy). Halo diameters were measured after cell lysis in the presence of propidium iodide (PI; 0.5 to 50 micrograms/ml) at pH 6.9 and 9. The ability of DNA to be rewound at 10-50 micrograms/ml of PI was impaired by X-irradiation and partly restored during 90-min post-irradiation incubation, indicating damage to the superhelical structure and its partial restoration. The exponential time constants for repair were 10.1 min (LY-S, 6 Gy), 11.2 min (LY-R, 12 Gy), and 20.3 min (LY-s, 12 Gy) when measured at pH 9. In X-irradiated (12 Gy) LY-S cells, slower restoration of DNA supercoiling was observed at pH9 than at pH 6.9. The presence of labile lesions at pH 9 did not prevent restoration of the higher-order DNA structure, as estimated from DNA rewinding at pH 6.9 in LY-S cells.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells. II. Repair

Kapiszewska

Szumiel

Lange

1994

Radiat Environ Biophys

View full text Add to dashboard Cite

show abstract

“…1974, Painter and Young 1980, Scott and Zampetti-Bosseler 1982 . A third property is a reduced capacity to rejoin DNA strand breaks which has been reported for L5178Y S/S cells (Ueno et al 1979a,b, Golden et al 1980, Evans et al . 1987, Wlodek and Hittelman 1987 .…”

Section: Introductionmentioning

confidence: 94%

An Ionizing Radiation-sensitive Mutant of CHO Cells: Irs-20. III. Chromosome Aberrations, DNA Breaks and Mitotic Delay

Stackhouse

Bedford

1994

International Journal of Radiation Biology

View full text Add to dashboard Cite

The ionizing radiation-sensitive mutant of CHO cells, irs-20, showed a defect in the rate and extent of rejoining of gamma-ray-induced DNA double-strand breaks (dsbs) compared with the parental CHO cells as measured using pulsed-field gel electrophoresis. Virtually all rejoined in wild-type cells but some 20-30% failed to rejoin in the mutant cells during a 5-h period after irradiation. An increased level of chromosome-type aberrations per unit dose was seen in irs-20 cells compared with wild-type cells irradiated during the G1 phase. For the irs-20 cells, about half the dose was required to produce the same chromosome-type aberration frequency. Chromatid-type aberrations were induced in G1-irradiated irs-20 cells at frequencies nearly the same as for chromosome types. For the parental wild-type CHO 10B2 cells, only chromosome types were seen. The distribution of aberrations among cells was not significantly different from Poisson for wild-type cells, but this was not the case for irs-20 cells where the overdispersion was highly significant. The mutant irs-20 cells displayed a much greater cell cycle delay per unit dose (about five-fold) in reaching mitosis after irradiation in G1 than the more radioresistant wild-type parental cells.

show abstract

“…The xrs, XR-1 and L5178Y-S mutants have been reported to show defective double-strand break (dsb) rejoining (Kemp et al, 1984;Giaccia et al, 1985;Evans et al, 1987;Wlodek & Hittelman, 1987) measured by neutral filter elution. The irs mutants have no measurable impairment of dsb rejoining, but irsl was found to have low repair fidelity compared with its parent line, V79, using plasmid reconstitution (Debenham et al, 1988b).…”

mentioning

confidence: 99%

A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line

Powell

Whitaker²,

Edwards³

et al. 1992

Br J Cancer

View full text Add to dashboard Cite

Summary DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells.The isolation of mutants with either increased or decreased sensitivity to cytotoxic agents has proved to be invaluable in the elucidation of mechanisms of action of such agents. The development of ionising radiation sensitive mutants had been largely limited to variants of Chinese hamster cells and the L5178Y murine lymphoma cell line (Sato & Hieda, 1979;Jeggo & Kemp, 1983;Giaccia et al., 1985;Jones et al., 1988). (Debenham et al., 1988b). Irs2 has been reported to exhibit radioresistant DNA synthesis (Jones et al., 1990). Low repair fidelity and radioresistant DNA synthesis are both reported features of A-T (Cox et al., 1986;Debenham et al., 1988a;Painter, 1981). A-T cells have no defect in dsb rejoining (Lehmann, 1982) with one reported exception (Coquerelle et al., 1987).In a previous report (McMillan & Holmes, 1991), the initial DNA damage, measured by neutral filter elution, was found to be the same in clone S40b and the parent line, MGH-Ul. To investigate the possibility of a repair defect in S40b we have now looked at DNA repair under two broad headings: (1) the rate and extent of dsb rejoining (2) repair fidelity. Dsb rejoining was assessed by neutral filter elution (NFE) and pulsed-field gel electrophoresis (PFGE). Repair fidelity was measured by plasmid reconstitution. Materials and methods Cell linesThe parent line, MGH-Ul, and a radiosensitivie clone, S40b, were grown in Ham's F12 medium supplemented with 10% foetal bovine serum, streptomycin (100 mg I') and penicillin (105 units I-) as an attached monolayer. All cultures were incubated at 37°C in 3% 02, 5% CO2 and 92% N2. The difference in cell survival following ionising radiation is shown in Figure 1 (from McMillan & Holmes, 1991 Pulsedfield gel electrophoresis (PFGE) PFGE was used as an alternative means of measuring DNA fragmentation using a CHEF-DRII system (Bio-Rad) as described previously (Whitaker & McMillan, 1992). Expontentially growing cells were labelled with 0.05 gCi ml-' C'4

show abstract

Deficiency in DNA repair in mouse lymphoma strain L5178Y-S.

Cited by 73 publications

References 22 publications

Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells. II. Repair

Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells. II. Repair

An Ionizing Radiation-sensitive Mutant of CHO Cells: Irs-20. III. Chromosome Aberrations, DNA Breaks and Mitotic Delay

A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line

Contact Info

Product

Resources

About