Deferoxamine promotes mesenchymal stem cell homing in noise‐induced injured cochlea through <scp>PI</scp>3K/<scp>AKT</scp> pathway

Peyvandi, Ali Asghar; Abbaszadeh, Hojjat-Allah; Roozbahany, Navid Ahmady; Pourbakht, Akram; Khoshsirat, Shahrokh; Niri, Hassan Haddadzade; Peyvandi, Hassan; Niknazar, Somayeh

doi:10.1111/cpr.12434

Cited by 37 publications

(26 citation statements)

References 49 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also found that BMSC-CM inhibited neuron and glial cell death and improved functional recovery. Cantinieaux D showed the use of BMSC-CM to prevent neuron apoptosis, activation of macrophages and motor improvement in the rat's SCI model due to neurotrophic secretions of BMSC, which were consistent with our results (14). Other researchers also found that the CM of some other types of stem cells, such as the umbilical cord and Embryonic stem cells, had similar effects to BMSC on spinal cord injury improvement (15).…”

Section: Discussionsupporting

confidence: 87%

“…Prescription of medications that decrease infl ammatory infi ltration and can inhibit the injury expansion (11). Methylprednisolone (MP) is a corticosteroid that inhibit lipid peroxidation and reduces infl ammatory cell and cytokine at the site of injury and improve the perfusion of the spinal cord and reduce the infl ux of neuronal calcium (12,13,14) The purpose of this experimental study is evaluation of the effect of BMSCs Condition Media and MP in TGF-β expression and functional recovery after acute spinal cord injury in adult wistar rat.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Khoshsirat¹,

Abbaszadeh²,

Ahrabi³

et al. 2018

BLL

Self Cite

View full text Add to dashboard Cite

The purpose of this experimental study is evaluation of the effect of BMSCs Condition Media and Methylprednisolone in TGF-β expression and functional recovery after acute spinal cord injury in adult wistar rat. MATERIAL AND METHODS: After an acute spinal cord injury, MP and BMSC-CM were injected intraperitoneally and the recovery rate was evaluated by BBB and narrow beam test. Real time PCR, H&E staining, cavity formation and stereology was done after 12 weeks post injury. RESULT: There were signifi cant differences in functional recovery between MP+CM group, relative to other groups. TGF-β1 expression decreased in MP+CM group compared to the control group. Cavity size in experimental groups decreased compared to the control group. The mean volume of spinal cord demonstrated a signifi cant increase in the MP+CM group in comparison to the other experimental groups. CONCLUSION: Simultaneous use of MP and BMSC-CM will improve recovery from the spinal cord injury, reduce infl ammation and improve functional recovery (Tab. 1, Fig. 8, Ref. 26).

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Khoshsirat¹,

Abbaszadeh²,

Ahrabi³

et al. 2018

BLL

Self Cite

View full text Add to dashboard Cite

show abstract

“…Activation of this pathway can be directly related to the increase of Ad-MSCs migration and homing of cells towards the injured tissues, as it had been demonstrated in many studies that chemokine and chemokine receptors have essential role in stem cell and cancer cell migration (Liu et al 2018;Mishan et al 2015Mishan et al , 2016. Similar to a study that DFX preconditioning significantly increased the homing ability of MSCs into injured area through increasing the expression of PI3K/AKT, HIF-1a and CXCR4 (Peyvandi et al 2018). Moreover, in a study, it was demonstrated that pre-treatment with VPA before transplantation enhances the therapeutic benefits of bone marrow MSCs (BMSCs) in terms of greater cell migration and better neurological outcomes through increasing the expression of CXCR4 after traumatic acute spinal cord injury (SCI) in animal models (Chen et al 2014).…”

Section: Discussionmentioning

confidence: 77%

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

et al. 2020

View full text Add to dashboard Cite

Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl 2) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of MAPK4 (associated with MAPK signaling pathway), INPP4B (associated with Inositol polyphosphate pathway), VEGF-A and VEGF-C (associated with cytokinecytokine receptor pathways), IL-8 and its receptor, CXCR2 (associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl 2 only upregulated VEGF-A and VEGF-C gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.

show abstract

“…In comparison with untreated MSCs, the homing of DFO‐treated MSCs in injured rat cochlea was increased. These effects of primed DFO MSCs were confirmed with a PI3K inhibitor (Peyvandi et al, 2018).…”

Section: Modulation Of Mscs For Therapeutic Purposementioning

confidence: 80%

Mesenchymal stromal cell‐derived extracellular vesicles as cell‐free biologics for the ex vivo expansion of hematopoietic stem cells

Budgude

Kale

Vaidya

2020

Cell Biology International

View full text Add to dashboard Cite

Hematopoietic stem cell transplantation (HSCT) is the ultimate choice of treatment for patients with hematological diseases and cancer. The success of HSCT is critically dependent on the number and engraftment efficiency of the transplanted donor hematopoietic stem cells (HSCs). Various studies show that bone marrow-derived mesenchymal stromal cells (MSCs) support hematopoiesis and also promote ex vivo expansion of HSCs. MSCs exert their therapeutic effect through paracrine activity, partially mediated through extracellular vesicles (EVs). Although the physiological function of EVs is not fully understood, inspiring findings indicate that MSC-derived EVs can reiterate the hematopoiesis, supporting the ability of MSCs by transferring their cargo containing proteins, lipids, and nucleic acids to the HSCs. The activation state of the MSCs or the signaling mechanism that prevails in them also defines the composition of their EVs, thereby influencing the fate of HSCs. Modulating or preconditioning MSCs to achieve a specific composition of the EV cargo for the ex vivo expansion of HSCs is, therefore, a promising strategy that can overcome several challenges associated with the use of naïve/unprimed MSCs. This review aims to speculate upon the potential role of preconditioned/primed MSC-derived EVs as "cell-free biologics," as a novel strategy for expanding HSCs in vitro.

show abstract

Deferoxamine promotes mesenchymal stem cell homing in noise‐induced injured cochlea through PI3K/AKT pathway

Abstract: Pre-conditioning of MSCs by DFO before transplantation can improve stem cell homing in the damaged cochlea through PI3K/AKT pathway activation.

Cited by 37 publications

References 49 publications

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

Mesenchymal stromal cell‐derived extracellular vesicles as cell‐free biologics for the ex vivo expansion of hematopoietic stem cells

Contact Info

Product

Resources

About