Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response

Moreira, Xoaquín; Sampedro, Luís; Zas, Rafael

doi:10.1016/j.envexpbot.2009.05.015

Cited by 92 publications

(114 citation statements)

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“…When seedlings were about 6 months old (25 th of April), the pots were divided into four groups, and the following treatments were applied: (i) spraying of seedlings with water and, 1 month later, inoculation with F. circinatum; (ii) spraying with an aqueous solution of MeJA (25 mM) and, 1 month later, inoculation with F. circinatum; (iii) spraying with MeJA (25 mM); and (iv) spraying water (control treatment). The MeJA dose was selected according to Moreira et al (2009). Each treatment consisted of 45 plants distributed among two blocks.…”

Section: Methodsmentioning

confidence: 99%

“…Evaluating methyl jasmonate for induction of resistance abietis (Heijari et al, 2005;Moreira et al, 2009 …”

Section: Introductionmentioning

confidence: 99%

“…There is growing evidence that exogenous applications of methyl jasmonate (MeJA) can enhance the levels of certain defensive compounds of plants and in consequence be used to trigger the defense mechanisms of trees (Moreira et al, 2009;Eyles et al, 2010). MeJA or (Z,E)-methyl 3-oxo-2-(2-pentyl) cyclopentane acetate is one of the mayor physiological active forms of jasmonates, and the most commonly studied elicitor in conifer species (Holopainen et al, 2009).…”

mentioning

confidence: 99%

“…MeJA or (Z,E)-methyl 3-oxo-2-(2-pentyl) cyclopentane acetate is one of the mayor physiological active forms of jasmonates, and the most commonly studied elicitor in conifer species (Holopainen et al, 2009). This compound is usually mixed with the surfactant Tween 20 at 0.1% (Hubber et al, 2005;Zeneli et al, 2006;Moreira et al, 2009) and directly applied by spraying or by brushing the plant. An aqueous solution of MeJA has been used to artificially induce defense responses of trees, through increasing the synthesis of terpenoid, phenolic and alkaloid compounds (Heijari et al, 2005;Zeneli et al, 2006) or by promoting the formation of traumatic resin ducts (Martin et al, 2002;Hudgins et al, 2004).…”

mentioning

confidence: 99%

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Evaluating methyl jasmonate for induction of resistance to Fusarium oxysporum, F. circinatum and Ophiostoma novo-ulmi

et al. 2012

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Damping off is probably the most common disease affecting seedlings in forest nurseries. In south-western Europe, the pitch canker and the Dutch elm disease cause relevant economic looses in forests, mostly in adult trees. The ability of the chemical plant elicitor methyl jasmonate (MeJA) to induce resistance in Pinus pinaster against Fusarium oxysporum and F. circinatum, and in Ulmus minor against Ophiostoma novo-ulmi was examined. In a first experiment, an aqueous solution of MeJA 5 mM was applied to P. pinaster seeds by immersion or spray, and different concentrations of MeJA (0, 0.1, 0.5, 1, 5 and 10 mM) were tested in seedlings before inoculations with F. oxysporum (10 5 and 10 7 spores mL -1 ). In a second experiment, 6-months-old P. pinaster seedlings were sprayed with 0 and 25 mM of MeJA, and later challenged with mycelium of F. circinatum. Finally, 4-year-old U. minor trees were sprayed with 0, 50 and 100 mM of MeJA and subsequently inoculated with O. novo-ulmi (10 6 spores mL -1 ). MeJA did not protect P. pinaster seeds and seedlings against F. oxysporum, probably because plants were too young for the physiological mechanisms responsible for resistance to be induced. Based on the morphological changes observed in the treated 6-months-old P. pinaster seedlings (reduction of growth and increased resin duct density), there is evidence that MeJA could have activated the mechanisms of resistance. However, 25 mM MeJA did not reduce plant mortality, probably because the spread of the virulent F. circinatum strain within the tree tissues was faster than the formation of effective defense responses. Based on the lack of phenological changes observed in the treated elms, there is no evidence that MeJA would cause induction of resistance. These results suggest that the use of MeJA to prevent F. oxysporum and F. circinatum in P. pinaster seedlings in nurseries and O. novo-ulmi in U. minor trees should be discarded.

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Section: Methodsmentioning

confidence: 99%

“…Evaluating methyl jasmonate for induction of resistance abietis (Heijari et al, 2005;Moreira et al, 2009 …”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluating methyl jasmonate for induction of resistance to Fusarium oxysporum, F. circinatum and Ophiostoma novo-ulmi

et al. 2012

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“…Each plant received 4.5±0.5 ml of solution applied to the foliage with a handheld sprayer to runoff. MJ dose and concentration were determined based on previous studies where we observed that exogenous application of MJ and real herbivory increased the concentration of resin in the stem and total phenolics in the needles by equivalent magnitudes (Moreira et al, 2009(Moreira et al, , 2012c). To avoid cross-contamination, the MJ treatment was applied in a separate greenhouse chamber where plants remained for a 24-h period to allow drying of the solution.…”

Section: Plant Materials and Experimental Designmentioning

confidence: 99%

Additive genetic variation in resistance traits of an exotic pine species: little evidence for constraints on evolution of resistance against native herbivores

2012

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The apparent failure of invasions by alien pines in Europe has been explained by the co-occurrence of native pine congeners supporting herbivores that might easily recognize the new plants as hosts. Previous studies have reported that exotic pines show reduced tolerance and capacity to induce resistance to those native herbivores. We hypothesize that limited genetic variation in resistance to native herbivores and the existence of evolutionary trade-offs between growth and resistance could represent additional potential constraints on the evolution of invasiveness of exotic pines outside their natural range. In this paper, we examined genetic variation for constitutive and induced chemical defences (measured as non-volatile resin in the stem and total phenolics in the needles) and resistance to two major native generalist herbivores of pines in cafeteria bioassays (the phloem-feeder Hylobius abietis and the defoliator Thaumetopoea pityocampa) using half-sib families drawn from a sample of the population of Pinus radiata introduced to Spain in the mid-19th century. We found (i) significant genetic variation, with moderate-to-high narrow-sense heritabilities for both the production of constitutive non-volatile resin and induced total phenolics, and for constitutive resistance against T. pityocampa in bioassays, (ii) no evolutionary trade-offs between plant resistance and growth traits or between the production of different quantitative chemical defences and (iii) a positive genetic correlation between constitutive resistance to the two studied herbivores. Overall, results of our study indicate that the exotic pine P. radiata has limited genetic constraints on the evolution of resistance against herbivores in its introduced range, suggesting that, at least in terms of interactions with these enemies, this pine species has potential to become invasive in the future. (2010)). For instance, the enemy release hypothesis predicts that exotic plants spread rapidly because they escape from their co-evolved natural enemies in the new range (Elton, 1958;Maron and Vilà, 2001). Accordingly, the evolution of increased competitive ability (EICA) hypothesis states that exotic plant species, with few specialized enemies in their new range, are more competitive than native species because they preferentially allocate resources to growth or reproduction instead of defences (Blossey and Nötzold, 1995). Although both hypotheses have been examined in a diverse array of terrestrial (Bossdorf et al.,

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Metabolomics ofPinusspp. in Response to Pinewood Nematode Infection

Rodrigues,

da Silva,

Vasconcelos

et al. 2023

Monitoring Forest Damage With Metabolomics Methods

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Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response

Cited by 92 publications

References 37 publications

Evaluating methyl jasmonate for induction of resistance to Fusarium oxysporum, F. circinatum and Ophiostoma novo-ulmi

Evaluating methyl jasmonate for induction of resistance to Fusarium oxysporum, F. circinatum and Ophiostoma novo-ulmi

Additive genetic variation in resistance traits of an exotic pine species: little evidence for constraints on evolution of resistance against native herbivores

Metabolomics ofPinusspp. in Response to Pinewood Nematode Infection

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