Defensin-Like Peptides and Their Antimicrobial Activity in Free-Form and Immobilized on Material Surfaces

Bruggeman, Marc; Ijakipour, Hanieh; Stamboulis, Artemis

doi:10.5772/intechopen.85508

Cited by 5 publications

(3 citation statements)

References 113 publications

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“…Defensins are usually characterized by 3 disulphide bonds; disulphide bonding analysis of our peptide Fig. 2 also showed the presence of three disulphide bridges [51][52][53] .…”

Section: Fig 1: Alignment Results Of Corvus Avbd7mentioning

confidence: 98%

Untitled

2020

IJPSR

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Antimicrobial peptides are considered as promising and potential next-generation antibiotics on account of their broad spectrum of antimicrobial activity and unique mode of microbial killing. For therapeutic application, it is essential to consider the hemolytic or cytotoxic property of AMPs along with antimicrobial function. In the current study, an avian beta-defensin 7 (AvBD7) gene was identified from Corvus splendens. The predicted amino acid sequence showed the presence of β defensin core motif and three disulphide bridges. We analysed the interaction of wild and mutant analogues of mature Corvus AvBD7 dimer with neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) that mimics mammalian membranes using molecular dynamics simulation studies. Hydrophobic residues at the N-terminus of each monomer were found to provide stability in the dimeric form. In both monomer and dimer, Asp3 and Asp4 interact with Tyr40 and Ser41 thereby helping in maintaining a claw shape at the C-terminus. During simulation, we found that the C-terminus claw region of wild dimer remained intact than its mutant analogs, and also wild AvBD7 exhibited a greater propensity to bind POPC membrane than its mutant analogs. Based on the membrane-peptide distance and number of hydrogen bonds formed between peptide and membrane, we conclude that the alanine mutant might be least hemolytic as it showed reduced propensity to bind to POPC membrane.

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“…Defensins are usually characterized by 3 disulphide bonds; disulphide bonding analysis of our peptide Fig. 2 also showed the presence of three disulphide bridges [51][52][53] .…”

Section: Fig 1: Alignment Results Of Corvus Avbd7mentioning

confidence: 98%

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2020

IJPSR

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“…DEFL peptides have been shown to have antimicrobial activity in vitro [ 44 ]. For these in vitro tests, we produced the At3g59930 peptide in Escherichia coli .…”

Section: Resultsmentioning

confidence: 99%

Characterization of an Arabidopsis Defensin-like Gene Conferring Resistance against Nematodes

Hawamda

Reichert

Ali

et al. 2022

Plants

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Arabidopsis contains 317 genes for defensin-like (DEFL) peptides. DEFLs have been grouped into different families based mainly on cysteine motifs. The DEFL0770 group contains seven genes, of which four are strongly expressed in roots. We found that the expression of these genes is downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii as revealed by RNAseq analysis. We have studied one gene of this group, At3g59930, in detail. A promoter::GUS line revealed that the gene is only expressed in roots but not in other plant organs. Infection of the GUS line with larvae of H. schachtii showed a strong downregulation of GUS expression in infection sites as early as 1 dpi, confirming the RNAseq data. The At3g59930 peptide had only weak antimicrobial activity against Botrytis cinerea. Overexpression lines had no enhanced resistance against this fungus but were more resistant to H. schachtii infection. Our data indicate that At3g59930 is involved in resistance to nematodes which is probably not due to direct nematicidal activity.

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“…On the other hand, development of a new group of anti-cancer drugs with low cytotoxicity to normal cells would be a major challenge as well. Therefore, AMPs are favorable potential candidates for cancer treatment [23]. Nevertheless, isolation of AMPs and their chemical synthesis could be complicated and expensive; therefore, it seems that production by recombination technology can be a promising pathway for to high yield production, with low cytotoxicity to healthy cells [24].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer Effect of Recombinant PI-Laterosporulin10 as a Novel Bacteriocin with Selective Cytotoxicity on Triple Negative Breast Cancer Cells

Rafizadeh

Reiisi

Saffar

et al. 2022

Int J Pept Res Ther

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Anti-microbial peptides (AMPs) are important factors of the innate immune system which perform a comprehensive spectrum of biological activities. The present study aims to examine the expression, puri cation, and cytotoxic effects of recombinant PI-laterosporulin10 (LS10) a defensing-like peptide. PI is a transmembrane peptide that helps to increase penetration and speci city of peptide targeting. PI-LS10 sequence was optimized and fused to a Xa protease site to achieve higher levels of expression. Then Xa-PI-LS10 was cloned into the pET-32a (+) vector and expressed in E. coli BL21 (DE3). The fusion protein with a molecular weight of about 25.3 kDa was analyzed using the SDS-PAGE. The maximum expression was observed 8 hours post-induction. After enzymatic cleavage, the purity of PI-LS10 was >300 µg/ ml as the nal yield. The recombinant PI-LS10 showed anti-cancer characteristics against breast cancer cell lines. MCF7 (non-triple negative) and MDA-MB-231 (triple negative) cells were treated with different concentrations of the PI-LS10 in order to evaluate their cytotoxic activity and e ciency in targeting triple negative cells. Our results suggest that PI-LS10 had a signi cant cytotoxic effect against breast cancer cells in comparison to the normal cell line. Triple negative breast cancer cell (MDA-MB-231) proliferation inhibited higher in 72 hours after treatment compare MCF7 cells. In conclusion, the PI-LS10 peptide was produced successfully and its favorable anti-cancer activity against a breast cancer cell line was con rmed.

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Defensin-Like Peptides and Their Antimicrobial Activity in Free-Form and Immobilized on Material Surfaces

Cited by 5 publications

References 113 publications

Untitled

Untitled

Characterization of an Arabidopsis Defensin-like Gene Conferring Resistance against Nematodes

Anti-cancer Effect of Recombinant PI-Laterosporulin10 as a Novel Bacteriocin with Selective Cytotoxicity on Triple Negative Breast Cancer Cells

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