Defense Responses and Changes in Symbiotic Gut Microflora in the Colorado Potato Beetle Leptinotarsa decemlineata under the Effect of Endophytic Bacteria from the Genus Bacillus

Sorokan, A. V.; Benkovskaya, G. V.; Благова, Д. К.; Maksimova, T. I.; Максимов, И. В.

doi:10.1134/s0022093018040063

Cited by 11 publications

(16 citation statements)

References 18 publications

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“…TS2, B. thuringiensis B-5351, and B. subtilis 26D significantly decreased defoliation caused by CPB; i.e., they showed antifeedant effect on CPB when they were used for potato plants treatment as well as attractiveness of plants for oviposition. It is particularly significant that B. thuringiensis B-5689 provided for a one-third increase in the mortality of L. decemlineata larvae as if under the influence of B. subtilis 26D under the laboratory conditions ( Sorokan et al, 2018 ) but had no significant impact on pest population density in the field, probably due to its low ability to invade internal plant tissues. We found that endophytic strains that showed insecticidal activity— B. thuringiensis B-5351 and B. thuringiensis B-6066 ( Sorokan et al, 2019 ), synthesizing Cry-protein, and B. subtilis 26D disturbing CPB microbiota ( Sorokan et al, 2016 )—effectively decreased abundance of L. decemlineata eggs, and young and old larvae and the defoliation level.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

et al. 2020

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Viral diseases and their damage causing significant loss to economically important crops have increased by several folds during the last decade. All the conventional approaches are not able to eradicate the viral infection. Therefore, there is a need to look for efficient and eco-friendly viral disease-preventive measures. The genomic material of the majority of deleterious viruses of higher plants is RNA. One of the possible measures to control viruses is the use of ribonucleases (RNases), which can cleave RNA in the viral genome. Based on this, we investigated the RNase activity of endophytic Bacillus spp., which can enrich in 10 3 –10 5 colony-forming units per gram of wet mass of aboveground part of potato plants. A high level of RNase activity was observed in the culture medium of Bacillus thuringiensis B-6066, Bacillus sp. STL-7, Bacillus sp. TS2, and Bacillus subtilis 26D. B. thuringiensis B-5351 had low RNase activity but high ability to colonize internal plant tissues, Bacillus sp. STL-7 with high RNase activity have relatively low number of cells in internal tissues of plants. B. thuringiensis B-6066, B. subtilis 26D, and Bacillus sp. TS stimulate RNase activity in potato plants for a long time after application. Strains with high ability to colonize internal plant tissues combined with high RNase activity reduced severity of viral diseases symptoms on plants and reduced the incidence of potato viruses M, S, and Y. It is worth noting that Bacillus spp. under investigation reduced the number of Leptinotarsa decemlineata Say. egg clusters and larvae on treated plants and showed antifeedant activity. This results in increase of potato productivity mainly in the fraction of major tubers. B. subtilis 26D and Bacillus sp. TS2 combining endophytic lifestyle, RNase, and antifeedant activity may become the basis for the development of biocontrol agents for plant protection.

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Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

et al. 2020

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“…Probably, spreading of infection was limited by through insecticidal properties of B. thuringiensis var. kurstaki В-5351 (Sorokan et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Strains under investigation also displayed insecticidal effect against Colorado potato beetle (Sorokan et al, 2018) and wheat aphid (Veselova et al, 2019), representing about 20 % from all aphids species which damaged potato plants (Ekaterinskaja et al, 2016) and act as carriers for viral particles. In addition to developing increased resistance against viral infection, bacterial barnases can participate in plant protection against other diseases, for example, tobacco plants from the late blight desease, as evidenced by high resistance of transgenic plants producing barnase (Natsoulis, Boeke, 1991).…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus bacteria with RNase activity in the resistance of potato plants to viruses

Бурханова¹,

Sorokan²,

Черепанова³

et al. 2019

Vestn. VOGiS

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Viral diseases annually cause significant crop losses and significantly reduce the quality of products, including potatoes, some of the most important crops. Currently, viruses cannot be controlled with chemical pesticides, since known antiviral compounds are teratogenic and hazardous to people’s health. Biocontrol agents based on endophytic microorganisms may be an alternative to them. Many strains of Bacillus produce ribonucleases (RNases). Our laboratory possesses a collection of bacteria that produce various metabolites and have RNase activity. The results showed that the inoculation of potato with B. subtilis 26D and B. thuringiensis increased the grain yield by 32–43 %. In addition, the treatment of potato plants with Bacillus spp. significantly reduced the infection of potato plants with virus M. The prevalence of the disease in potato plants was significantly reduced from 60 % in the control to 18 % (B. subtillis 26D) and 25–33 % (B. thuringiensis) in the inoculated plants. Similarly, the infection index decreased from 14 in the control to 1 in the inoculated plants. The further study of molecular mechanisms related to bacterial induction of plant defense reactions in response to viral infections will lead to a better understanding of stress resistance problems. The endophytic microorganisms studied in this report may become the basis for the creation of biological agents for plant protection.

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“…kurstaki-5351 and Bacillus subtilis 26D were antibiotic to colorado potato beetle Leptinotarsa decemlineata. The strains suppressed phenoloxidase and acetylcholinesterase activities, and affected the symbiotic bacteria in beetle's gut (Sorokan et al 2018). Other endophytic bacteria with entomopathogenic properties such as Pseudomonas spp.…”

Section: Biotic Stress Mitigationmentioning

confidence: 99%

Microbiome for sustainable agriculture: a review with special reference to the corn production system

et al. 2021

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Microbial diversity formed by ages of evolution in soils plays an important role in sustainability of crop production by enriching soil and alleviating biotic and abiotic stresses. This diversity is as an essential part of the agro-ecosystems, which is being pushed to edges by pumping agrochemicals and constant soil disturbances. Consequently, efficiency of cropping system has been decreasing, aggravated further by the increased incidence of abiotic stresses due to changes in climatic patterns. Thus, the sustainability of agriculture is at stake. Understanding the microbiota inhabiting phyllosphere, endosphere, spermosphere, rhizosphere, and non-rhizosphere, and its utilization could be a sustainable crop production strategy. This review explores the available information on diversity of beneficial microbes in agricultural ecosystem and synthesizes their commercial uses in agriculture. Microbiota in agro-ecosystem works by nutrient acquisition, enhancing nutrient availability, water uptake, and amelioration of abiotic and abiotic stresses. External application of such beneficial microbiota or microbial consortia helps in boosting plant growth and provides resistance to drought, salinity, heavy metal, high-temperature and radiation stress in various crop plants. These have been instrumental in enhancing tolerance to diseases, insect pest and nematodes in various cropping system. However, studies on the microbiome in revolutionary production systems like conservation agriculture and protected cultivation, which use lesser agrochemicals, are limited and if exploited can provide valuable input in sustainable agriculture production.

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Defense Responses and Changes in Symbiotic Gut Microflora in the Colorado Potato Beetle Leptinotarsa decemlineata under the Effect of Endophytic Bacteria from the Genus Bacillus

Cited by 11 publications

References 18 publications

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

Endophytic Bacillus bacteria with RNase activity in the resistance of potato plants to viruses

Microbiome for sustainable agriculture: a review with special reference to the corn production system

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