Defense Reaction inMedicago sativa:A Gene Encoding a Class 10 PR Protein Is Expressed in Vascular Bundles

A Pathogen-Induced Chitin-Binding Protein Gene from Pepper: Its Isolation and Differential Expression in Pepper Tissues Treated with Pathogens, Ethephon, Methyl Jasmonate or Wounding

“…The in situ hybridization experiments with infected leaf tissues demonstrated that the CACBP1 mRNA accumulation occurs massively within the pathogen penetration sites and phloem cells. Our results were consistent with the data obtained for other PR protein genes, such as PR-1, chitinase, PR-10, win (wound induced gene) and thionin, which exhibited strong expression in vascular bundles (Stanford et al 1989, Zhu et al 1993, Breda et al 1996, Lee et al 2000a, Lee et al 2000b, Lee et al 2000c) and penetration sites (Somssich et al 1998).…”

Molecular Cloning and Characterization of a Novel Jasmonate Inducible Pathogenesis-Related Class 10 Protein Gene, JIOsPR10, from Rice (Oryza sativa L.) Seedling Leaves

“…Most PR10 members have been shown to be transcriptionally activated upon pathogen attack or after treatment with fungal elicitors, like the sorghum PR10 (13), the cotton PR10 (14), the bean Ypr10 (28), the Medicago sativa MsPR10-1 (30), and the apple Ypr10 (41), to name a few. It is interesting to note that in the case of sorghum, the PR10 transcript was found to accumulate much rapidly (at 6 h post-inoculation), followed by an increase and gradual decrease till the end of the experiment in the non-host resistance to Cochliobolus heterostrophus, rather than in the host resistant reaction to Collectotrichum sublineolum, where a delayed accumulation of the PR10 mRNA (at 36 h post-inoculation) that increased and remained at an almost constant level till the end of the experiment (13).…”

“…Multiple hybridizing bands of varying intensities and sizes in the different digested DNA samples were found at low stringency (LOW), which suggested that there might be multiple copies of PR10 genes in rice. One of the characteristics of PR10 genes is that "they are almost invariably part of gene families" (13,30). However, under a high stringency (HIGH) wash, a single major distinct band, along with a few faintly hybridizing bands were observed.…”

Amino acid sequence of proteins induced in Fe-deficient stressed alfalfa (Medicago sativaL.) roots

“…I was compared with the primary structure of pea ABRI7 (Iturriaga et al 1994) and kidney bean with that of PvPRI (Walter et al 1990). Peptide No.2 was compared with those of alfalfa SRGI (Truesdell and Dickman 1997), alfalfa MsPRlO-1 (Breda et al 1996) and kidney bean PvPR2 (Walter et al 1990). Peptide No.3 was compared with the primary structure of pea (White and Zilinskas 1991) and maize (Canon and Scandalios 1989) cytosolic Cu,Zn-SOD.…”

“…Complete amino acid primary structure of SRG 1 and MsPRIO-I is shown in the figure. proteins; ABR17 from pea (Iturriaga et al 1994) and pathogenesis-related (PR) protein; PvPRl from kidney bean (Walter et al 1990), respectively. Peptide 2 was considered to correspond to the disease resistance response protein; SRG 1 (Truesdell and Dickaman 1997) or pathogenesis-related protein; MsPRlO-l (Breda et al 1996) from alfalfa. Peptide 2 from alfalfa was highly homologous (81 %) to PvPR2 (PR protein) from kidney bean.…”