Defense mechanisms of the peritoneal cavity

Broche, Felix; Tellado, Jose M.

doi:10.1097/00075198-200104000-00009

Cited by 82 publications

(66 citation statements)

References 49 publications

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“…More than 90% of CD11b + cells were positive for the macrophage-specific Ag F4/80, which is expressed by mature macrophages (44), indicating a defect in the migration of those cells to the peritoneum. Using a model of thioglycolateelicited peritonitis to study macrophage emigration described previously (10,11), we show in this study that macrophage emigration to lymph nodes is reduced after LPS challenge in the absence of Cox-2. Cox-2 2/2 macrophages were defective with regard to migration toward CCR2-and CCR5-binding chemokines, as were cox-2 +/+ macrophages treated with Cox-2-specific inhibitors, whereas cox-2 2/2 bone marrow-derived DCs migrated normally in response to MIP-3b (a ligand of the CCR7 receptor).…”

Section: Discussionsupporting

confidence: 61%

“…Macrophages are able to emigrate from the peritoneal cavity to adjacent lymph nodes in response to LPS (11). To address whether emigration of cox-2 2/2 peritoneal macrophages to lymph nodes was also affected, we transferred peritoneal-elicited macrophages freshly isolated from cox-2 +/+ and cox-2 2/2 mice into the peritoneal cavity of C57BL/6 mice.…”

Section: Resultsmentioning

confidence: 99%

“…Egression of leukocytes, including neutrophils, monocytes, and dendritic cells (DCs), is indispensable during the adaptive immune response to transport and present Ags in secondary immune organs (5)(6)(7)(8). Furthermore, clearance of inflammatory conditions, such as atherosclerosis (5,9) or acute peritonitis, occurs, in part, as a result of macrophage emigration (10,11). Nonetheless, very little is known about the molecular basis controlling this mechanism.…”

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confidence: 99%

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Cyclooxygenase-2 Deficiency in Macrophages Leads to Defective p110γ PI3K Signaling and Impairs Cell Adhesion and Migration

Díaz-Muñoz

Osma-García

Íñiguez

et al. 2013

The Journal of Immunology

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Cyclooxygenase (Cox)-2 dependent PGs modulate several functions in many pathophysiological processes, including migration of immune cells. In this study, we addressed the role of Cox-2 in macrophage migration by using in vivo and in vitro models. Upon thioglycolate challenge, CD11b+ F4/80+ macrophages showed a diminished ability to migrate to the peritoneal cavity in cox-2−/− mice. In vivo migration of cox-2−/− macrophages from the peritoneal cavity to lymph nodes, as well as cell adhesion to the mesothelium, was reduced in response to LPS. In vitro migration of cox-2−/− macrophages toward MCP-1, RANTES, MIP-1α, or MIP-1β, as well as cell adhesion to ICAM-1 or fibronectin, was impaired. Defects in cell migration were not due to changes in chemokine receptor expression. Remarkably, cox-2−/− macrophages showed a deficiency in focal adhesion formation, with reduced phosphorylation of paxillin (Tyr188). Interestingly, expression of the p110γ catalytic subunit of PI3K was severely reduced in the absence of Cox-2, leading to defective Akt phosphorylation, as well as cdc42 and Rac-1 activation. Our results indicate that the paxillin/p110γ-PI3K/Cdc42/Rac1 axis is defective in cox-2−/− macrophages, which results in impaired cell adhesion and migration.

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Section: Discussionsupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cyclooxygenase-2 Deficiency in Macrophages Leads to Defective p110γ PI3K Signaling and Impairs Cell Adhesion and Migration

Díaz-Muñoz

Osma-García

Íñiguez

et al. 2013

The Journal of Immunology

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“…Antibakteriyel özellikler içerisinde opsonizasyon, kemotaksis, fagositoz ve öldürme fonksiyonları ön plandadır (9). Bakterinin öldürül-mesine kadar olan aşamada kompleman bağlama, fagositoz yapan hücrelere bağlanma, antiprotein antikor ve antipolisakkarit antikor olarak IgG'nin önemi iyi bilinmektedir.…”

Section: Discussionunclassified

Sürekli Ayaktan Periton Diyaliz Hastalarında Peritonit ve Serum-Diyalizat İmmünglobulin-G Subgrup Düzeyleri İlişkisi

Sarıkaya¹,

Şahintürk²,

İnci³

2017

Turk Neph Dial Transpl

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ÖzAMAÇ: Peritonit, Sürekli Ayaktan Periton Diyaliz (SAPD) tedavisinin en önemli komplikasyonudur. Peritoneal savunma sisteminin önemli elementi IgG ve subgruplarıdır. IgG subgrupları eksikliği tekrarlayan infeksiyonlara eğilimle sonuçlanması nedeniyle serum ve diyalizatta IgG subgruplarının düzeylerinin peritonit sıklığı ile ilişkisinin araştırılması amaçlandı. GErEÇ ve yÖntEMLEr:Çalışmaya 30 SAPD hastası alındı. Hastalar yıllık peritonit sıklığına göre iki gruba ayrıldı. Yılda iki veya daha fazla sayıda peritonit atağı geçiren hastalar high peritonitis index (HPI), yılda bir veya hiç peritonit geçirmeyenler ise low peritonitis index (LPI) olarak gruplandırıldı. 17 (%56,67) hasta LPI, 13 (%43,33) hasta HPI grubuna dahil edildi. İki grup IgG subgrup düzeyi açısından karşılaştırıldı.BULGULAr: Her iki grupta serum IgG subgrupları karşılaştırıldığında anlamlı fark saptanmadı (p IgG1:0,86, IgG2:0,93, IgG3:0,4, IgG4:0,67, total IgG:0,80 mg/ml). Diyalizat IgG subgruplarının HPI grubunda anlamlı düzeyde yüksek olduğu görüldü (p IgG1:0,006, IgG2:0,01, IgG3:0,007, IgG4:0,01, total IgG:0,003 mg/ml). HPI grubunda diyalizat total protein, globulin ve albumin kayıp düzeyleri LPI grubuna göre belirgin olarak yüksek bulundu (sırasıyla p=0,39, 0,26, 0,01).SOnUÇ: HPI olgularında IgG subgrupları düzeylerinin LPI olgularına göre yüksek olmasının nedeni, tekrarlayan peritonitler sonucunda periton membranındaki yapısal değişikliklere bağlı artmış makro molekül kaybı ve gelişen inflamatuvar bir yanıt olabilir. Sonuçlarımız IgG subgrup düzeyleri eksikliğinin peritonit gelişiminde önemli bir rol oynamadığını düşündürmektedir.AnAhtAr SÖzcükLEr: Sürekli ayaktan periton diyalizi, Peritonit, IgG subgrupları ABStrAct OBJEctIVE: Peritonitis is the most important complication of Continuous Ambulatory Peritoneal Dialysis (CAPD) treatment. The most powerful defending elements of peritoneal fluid are IgG and the IgG subclasses. Low levels of IgG subclasses result in recurrent infections. We therefore aimed to investigate the peritonitis frequency and serum-dialysate IgG subclass level relationship. MAtErIAL and MEthODS:A total of 30 CAPD patients were included in this study. The patients were divided into two groups according to their peritonitis frequency. Patients with two or more peritonitis attack were grouped as High Peritonitis Index (HPI) and those with one or less peritonitis attack as Low Peritonitis Index (LPI). 17 (56.67%) patients were in the LPI and 13 (43.33%) patients in the HPI group. These two groups were compared for their IgG subclass levels.rESULtS: There was no significant difference for serum IgG and subclass levels (p IgG1:0.86, IgG2:0.93, IgG3:0.4, IgG4:0.67, total IgG:0.80 mg/ml). Dialysate IgG and subclass levels were significantly higher in the HPI group (p IgG1:0.006, IgG2:0.01, IgG3:0.007, IgG4:0.01, total IgG:0.003 mg/ml). The loss of total protein, albumin and globulin in the dialysate was significantly higher in the HPI group (respectively p=0.39, 0.26, 0.01).cOncLUSIOn: Structural changes in the peritoneal membra...

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“…The peritoneal cavity contains resident and migratory cell populations, which play crucial roles in the local defensive response against bacterial invasion (Broche & Tellado 2001). There are many specialized defense mechanisms connected with immunity of peritoneal cavity.…”

Section: Discussionmentioning

confidence: 99%

A comparative study of local defense spots within the peritoneal cavity in Japanese quails,Coturnix coturnix japonica(Galliformes, Phasianidae) and Wistar rats,Rattus norvegicus(Rodentia, Muridae)

Saydam

Eren

2012

Italian Journal of Zoology

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The local defense spots (milky spot) are found on the greater omentum but also in other peritoneal regions, as well as on the pleura and pericardium. Milky spots are small accumulations of leukocytes scattered around the capillaries. The most frequent cells in milky spots are the macrophages, followed by lymphocytes and mast cells. In this study, the structure of milky spots of quails and rats after antigenic stimulation with bovine serum albumin were examined at the light microscope levels by applying conventional histochemical and immunohistochemical methods. The parietal peritoneum and mesentery samples from quails and parietal peritoneum, mesentery and omentum majus samples from rats were collected. Local defense spots were seen as local lymphoid cell aggregations in the omentum, mesentery and parietal peritoneum in rats, whereas in quails they were seen only in the mesentery. After the bovine serum albumin injection, the dimensions of spots were at their biggest 6 hours after injection (p < 0.001) in the rat and 1 hour after injection (p < 0.01) in the quail. Lymphocytes, macrophages (MHC II positive cells in quail), plasma cells and mast cells were detected within the aggregates of both rat and quails. In conclusion, it was determined that the quails have local defense spots at the mesentery like the milky spots of rats. In addition, the present study findings confirm that local defense spots react to antigenic stimulation by innate and adaptive immunity in rats and quails.

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Defense mechanisms of the peritoneal cavity

Cited by 82 publications

References 49 publications

Cyclooxygenase-2 Deficiency in Macrophages Leads to Defective p110γ PI3K Signaling and Impairs Cell Adhesion and Migration

Cyclooxygenase-2 Deficiency in Macrophages Leads to Defective p110γ PI3K Signaling and Impairs Cell Adhesion and Migration

Sürekli Ayaktan Periton Diyaliz Hastalarında Peritonit ve Serum-Diyalizat İmmünglobulin-G Subgrup Düzeyleri İlişkisi

A comparative study of local defense spots within the peritoneal cavity in Japanese quails,Coturnix coturnix japonica(Galliformes, Phasianidae) and Wistar rats,Rattus norvegicus(Rodentia, Muridae)

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