Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Man, Mingwu; Zhu, Yaqian; Liu, Lulu; Luo, Lei; Han, Xinpei; Qiu, Lu; Li, Fuguang; Ren, Maozhi; Xing, Yanmei

doi:10.3390/ijms232012217

Cited by 11 publications

(7 citation statements)

References 182 publications

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“…Cotton belongs to the genus Gossypium in the family Malvaceae and produces the most important textile fiber, as it contributes about 35% of total nature fiber for the textile industry [ 1 ], and also serves as one of the sources of edible oil and livestock feed [ 2 ]. Among them, upland cotton Gossypium hirsutum is the most widespread and encompasses 95% of global cotton production [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Shan,

Wang,

Zhao

et al. 2024

BMC Biol

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Background Verticillium wilt, caused by the fungus Verticillium dahliae, is a soil-borne vascular fungal disease, which has caused great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from the seed of Verticillium wilt-resistant Gossypium hirsutum cultivar “Zhongzhimian No. 2.” Results The strain KRS010 has a broad-spectrum antifungal activity to various pathogenic fungi as Verticillium dahliae, Botrytis cinerea, Fusarium spp., Colletotrichum spp., and Magnaporthe oryzae, of which the inhibition rate of V. dahliae mycelial growth was 73.97% and 84.39% respectively through confrontation test and volatile organic compounds (VOCs) treatments. The strain was identified as Bacillus altitudinis by phylogenetic analysis based on complete genome sequences, and the strain physio-biochemical characteristics were detected, including growth-promoting ability and active enzymes. Moreover, the control efficiency of KRS010 against Verticillium wilt of cotton was 93.59%. After treatment with KRS010 culture, the biomass of V. dahliae was reduced. The biomass of V. dahliae in the control group (Vd991 alone) was 30.76-folds higher than that in the treatment group (KRS010+Vd991). From a molecular biological aspect, KRS010 could trigger plant immunity by inducing systemic resistance (ISR) activated by salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Its extracellular metabolites and VOCs inhibited the melanin biosynthesis of V. dahliae. In addition, KRS010 had been characterized as the ability to promote plant growth. Conclusions This study indicated that B. altitudinis KRS010 is a beneficial microbe with a potential for controlling Verticillium wilt of cotton, as well as promoting plant growth.

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Section: Introductionmentioning

confidence: 99%

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Shan,

Wang,

Zhao

et al. 2024

BMC Biol

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“…Cotton is an important economic crop worldwide, contributing approximately 35% of the world's total natural fiber to the textile industry. Additionally, it serves as a source of edible oil and livestock feed [1]. Verticillium wilt is a significant vascular soil-borne disease affecting cotton.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Verticillium dahliae from Soil Using LAMP-CRISPR/Cas12a Technology

Fang,

Liu,

Zhao

et al. 2024

IJMS

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Cotton Verticillium wilt is mainly caused by the fungus Verticillium dahliae, which threatens the production of cotton. Its pathogen can survive in the soil for several years in the form of microsclerotia, making it a destructive soil-borne disease. The accurate, sensitive, and rapid detection of V. dahliae from complex soil samples is of great significance for the early warning and management of cotton Verticillium wilt. In this study, we combined the loop-mediated isothermal amplification (LAMP) with CRISPR/Cas12a technology to develop an accurate, sensitive, and rapid detection method for V. dahliae. Initially, LAMP primers and CRISPR RNA (crRNA) were designed based on a specific DNA sequence of V. dahliae, which was validated using several closely related Verticillium spp. The lower detection limit of the LAMP-CRISPR/Cas12a combined with the fluorescent visualization detection system is approximately ~10 fg/μL genomic DNA per reaction. When combined with crude DNA-extraction methods, it is possible to detect as few as two microsclerotia per gram of soil, with the total detection process taking less than 90 min. Furthermore, to improve the method’s user and field friendliness, the field detection results were visualized using lateral flow strips (LFS). The LAMP-CRISPR/Cas12a-LFS system has a lower detection limit of ~1 fg/μL genomic DNA of the V. dahliae, and when combined with the field crude DNA-extraction method, it can detect as few as six microsclerotia per gram of soil, with the total detection process taking less than 2 h. In summary, this study expands the application of LAMP-CRISPR/Cas12a nucleic acid detection in V. dahliae and will contribute to the development of field-deployable diagnostic productions.

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“…GbGSTU7 positively regulates resistance to Fusarium wilt in Gossypium barbadense, whereas its silencing significantly reduces glutathione peroxidase activity in vivo and increases the incidence of Fusarium cotton wilt [13]. The GhRLPGSO1like genes, including GhRLP44, GhRLP6, and GhRLP34, might play a role in resistance to Fusarium cotton wilt [14]. These genes are related to mitogen-activated protein kinase (MAPK), transcription factors (TFs), and glutathione transferases (GSTs) [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…The GhRLPGSO1like genes, including GhRLP44, GhRLP6, and GhRLP34, might play a role in resistance to Fusarium cotton wilt [14]. These genes are related to mitogen-activated protein kinase (MAPK), transcription factors (TFs), and glutathione transferases (GSTs) [12][13][14][15]. For more than a century, R genes have been identified in tomato (Solanum lycopersicum), such as I, I-2, and I-3, which are related to resistance to the Fusarium wilt caused by soil-borne fungus F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Characterization and Gene Changes Induced by Fusarium solani in Sweetpotato Roots

Zhang

Luo

Tang

et al. 2023

Genes

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Sweetpotato (Ipomoea batatas) is an important root crop that is infected by Fusarium solani in both seedling and root stages, causing irregular black or brown disease spots and root rot and canker. This study aims to use RNA sequencing technology to investigate the dynamic changes in root transcriptome profiles between control check and roots at 6 h, 24 h, 3 days, and 5 days post-inoculation (hpi/dpi) with F. solani. The results showed that the defense reaction of sweetpotato could be divided into an early step (6 and 24 hpi) without symptoms and a late step to respond to F. solani infection (3 and 5 dpi). The differentially expressed genes (DEGs) in response to F. solani infection were enriched in the cellular component, biological process, and molecular function, with more DEGs in the biological process and molecular function than in the cellular component. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the main pathways were metabolic pathways, the biosynthesis of secondary metabolites, and carbon metabolism. More downregulated genes were identified than upregulated genes in the plant–pathogen interaction and transcription factors, which might be related to the degree of host resistance to F. solani. The findings of this study provide an important basis to further characterize the complex mechanisms of sweetpotato resistance against biotic stress and identify new candidate genes for increasing the resistance of sweetpotato.

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Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Cited by 11 publications

References 182 publications

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Rapid and Sensitive Detection of Verticillium dahliae from Soil Using LAMP-CRISPR/Cas12a Technology

Transcriptome Characterization and Gene Changes Induced by Fusarium solani in Sweetpotato Roots

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