Defense, Fast and Slow: Activation of Different MAPK Pathways in Response to Wounding

Hõrak, Hanna

doi:10.1105/tpc.20.00282

Cited by 8 publications

(8 citation statements)

References 4 publications

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“…The ?-MKK3-MPK8 module is originally induced by wounding towards homeostasis of reactive oxygen species (ROS) [17]. Jasmonic acid production was also shown to be amongst the several wound responses [57,58]. As plants in the present study are not wounded, then the occurrence of the MKK3-MPK8 module can be due to ABA accumulation under salt stress as previously described [59].…”

Section: Mapk-core Analysissupporting

confidence: 61%

Stepwise signal transduction cascades under salt stress in leaves of wild barley (Hordeum spontaneum)

Abulfaraj

2020

Biotechnology & Biotechnological Equipment

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The present study aims at the use of transcriptomic data of wild barley (Hordeum spontaneum) under salt stress in detecting regulated metabolites and crosstalking signal transduction pathways that help plants withstand harsh conditions. The studied pathways involved those emphasizing the roles of phytohormones auxin and abscisic acid (ABA) in conferring stress tolerance and adaptation. Of which, tryptophan-core pathways include metabolites that trigger auxinresponsive genes towards downstream signal transduction cascades and better plant growth. Analysis of 'MAPK signalling' pathway indicated active participation of several MAPK modules in receiving and delivering signals under salt stress towards appropriate downstream biological decisions. The results indicated that one enriched MEKK1 module, e.g. MEKK1-MKK2-MPK4/6, and two MKK3 modules, e.g. MEKK17/18-MKK3-MPK1/2/7/14 and ?-MKK3-MPK8, in this wild plant can effectively participate in salt stress adaptation and tolerance as well as homeostasis of reactive oxygen species (ROS). ABA seems to be a main player in the leaves of H. spontaneum in triggering signal transduction via MAPK modules. MKK7 was shown to be repressed under salt stress. This MAPK disturbs normal production, signalling and distribution of auxin, thus, enforces the plant to perform better under salt stress. In conclusion, the present study addresses signal transduction pathways that might justify the ability of this wild plant to tolerate salt stress.

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Section: Mapk-core Analysissupporting

confidence: 61%

Stepwise signal transduction cascades under salt stress in leaves of wild barley (Hordeum spontaneum)

Abulfaraj

2020

Biotechnology & Biotechnological Equipment

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“…In cotton, MKK4 regulates ABA, gibberellic acid, and hydrogen peroxide signaling [ 71 ]. MKK5 itself is involved in responses to salt and drought stress [ 72 ] and both MKK4 and MKK5 play a vital role in response to wounding and herbivory attack by promoting ethylene production via the transcriptional upregulation of 1-Aminocyclopropane-1-Carboxylate Synthase in Arabidopsis [ 73 ]. By contrast, BnaMKK4 only responded to cold stress, and BnaMKK5 did not respond to any of the stresses tested here, which demonstrated that MKKs genes have different patterns in stress response in different plant species.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

Wang

Wan

Meng

et al. 2021

IJMS

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Mitogen-activated protein kinase (MAPK) cascades are common and conserved signal transduction pathways and play important roles in various biotic and abiotic stress responses and growth and developmental processes in plants. With the advancement of sequencing technology, more systematic genetic information is being explored. The work presented here focuses on two protein families in Brassica species: MAPK kinases (MKKs) and their phosphorylation substrates MAPKs. Forty-seven MKKs and ninety-two MAPKs were identified and extensively analyzed from two tetraploid (B. juncea and B. napus) and three diploid (B. nigra, B. oleracea, and B. rapa) Brassica species. Phylogenetic relationships clearly distinguished both MKK and MAPK families into four groups, labeled A–D, which were also supported by gene structure and conserved protein motif analysis. Furthermore, their spatial and temporal expression patterns and response to stresses (cold, drought, heat, and shading) were analyzed, indicating that BnaMKK and BnaMAPK transcript levels were generally modulated by growth, development, and stress signals. In addition, several protein interaction pairs between BnaMKKs and C group BnaMAPKs were detected by yeast two-hybrid assays, in which BnaMKK3 and BnaMKK9 showed strong interactions with BnaMAPK1/2/7, suggesting that interaction between BnaMKKs and C group BnaMAPKs play key roles in the crosstalk between growth and development processes and abiotic stresses. Taken together, our data provide a deeper foundation for the evolutionary and functional characterization of MKK and MAPK gene families in Brassica species, paving the way for unraveling the biological roles of these important signaling molecules in plants.

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“…MYC2 protein was degraded at 20 min and then recovered ( Figure 3 E,F). This post-translational modification may be regulated by kinases as numerous kinases are activated during wounding [ 45 , 46 , 47 ]. This means that MYC2 may be negatively regulated by one of the activated kinases during early wounding.…”

Section: Discussionmentioning

confidence: 99%

A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded Arabidopsis Protoplasts

Son

Kwon

2021

Plants

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Wounding is a constant threat to plant survival throughout their lifespan; therefore, understanding the biological responses to wounds at the cellular level is important. The protoplast system is versatile for molecular biology, however, no wounding studies on this system have been reported. We established a new approach for wounding research using mechanically damaged Arabidopsis mesophyll protoplasts. Wounded protoplasts showed typical wounding responses, such as increased MPK6 kinase activity and upregulated JAZ1 expression. We also assessed expression profiles and protein stability of the basic helix-loop-helix transcription factor MYC2 in wounded protoplasts. Promoter activity, gene expression, and protein stability of MYC2 were compromised, but recovered in the early stage of wounding. In the late stage, the promoter activity and expression of MYC2 were increased, but the protein stability was not changed. According to the results of the present study, this new cell-based approach will be of use in various molecular studies on plant wounding.

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Defense, Fast and Slow: Activation of Different MAPK Pathways in Response to Wounding

Cited by 8 publications

References 4 publications

Stepwise signal transduction cascades under salt stress in leaves of wild barley (Hordeum spontaneum)

Stepwise signal transduction cascades under salt stress in leaves of wild barley (Hordeum spontaneum)

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded Arabidopsis Protoplasts

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