Defence sugarcane glycoproteins disorganize microtubules and prevent nuclear polarization and germination of Sporisorium scitamineum teliospores

Sánchez-Elordi, Elena; Baluška, Frantǐsek; Echevarrı́a, Cristina; Vicente, Carlos; Legaz, Marı́a-Estrella

doi:10.1016/j.jplph.2016.05.022

Cited by 12 publications

(4 citation statements)

References 66 publications

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“…Some studies have demonstrated that resistance to S. scitamineum is associated with chemical barriers, such as the presence of phenylpropanoids, flavonoids (Fontaniella et al 2002;de Armas et al 2007), free and conjugated glycoproteins, and an increase of polyamines in sugarcane buds (Millanes et al 2008). Glycoproteins prevent the correct arrangement of microtubules and cause nuclear fragmentation defects, contributing to germinative failure of teliospores (Sánchez-Elordi et al 2016). Likewise, resistance may be associated with the presence of trichomes, and the quantity of scales present in buds (da Gloria et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

Peters

Carvalho

Vilhena

et al. 2016

Planta

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Smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. Sporisorium scitamineum is the causal agent of sugarcane smut disease. In this study, we characterized sugarcane reactive oxygen species (ROS) metabolism in response to the pathogen in smut-resistant and -susceptible genotypes. Sporisorium scitamineum teliospore germination and appressorium formation coincided with HO accumulation in resistant plants. The superoxide dismutase (SOD) activity was not responsive in any of the genotypes; however, a higher number of isoenzymes were detected in resistant plants. In addition, related to resistance were lipid peroxidation, a decrease in catalase (CAT), and an increase in glutathione S-transferase (GST) activities and an earlier transcript accumulation of ROS marker genes (CAT3, CATA, CATB, GST31, GSTt3, and peroxidase 5-like). Furthermore, based on proteomic data, we suggested that the source of the increased hydrogen peroxide (HO) may be due to a protein of the class III peroxidase, which was inhibited in the susceptible genotype. HO is sensed and probably transduced through overlapping systems related to ascorbate-glutathione and thioredoxin to influence signalling pathways, as revealed by the presence of thioredoxin h-type, ascorbate peroxidase, and guanine nucleotide-binding proteins in the infected resistant plants. Altogether, our data depicted the balance of the oxidative burst and antioxidant enzyme activity in the outcome of this interaction.

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Section: Introductionmentioning

confidence: 99%

Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

Peters

Carvalho

Vilhena

et al. 2016

Planta

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“…Microtubules-actin interactions regulate important processes in which dynamic cellular asymmetries need to be established such as cell motility, neuronal pathfinding, cellular wound healing, cell division, and cortical flow [57]. The presence of tubulin has also been demonstrated by immunohistochemical techniques in S. scitamineum cells [58].…”

Section: Cytoskeleton Reorganization In S Scitamineum Cells In Respomentioning

confidence: 99%

“…It is clear that cytoskeleton plays a crucial role in polarity establishment in fungal cells during germination: microtubules support nuclei division and long-distance-transport functions in filamentous fungi, whereas actin microfilaments are required for localized targeting events [61]. Microtubule organization in S. scitamineum teliospores seems to be crucial for a successful germination [58]. S. scitamineum secretes its own arginase, which activates a signal transduction cascade that accelerates teliospore germination when it binds to its cell wall.…”

Section: Cytoskeleton Reorganization In S Scitamineum Cells In Respomentioning

confidence: 99%

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Sánchez-Elordi¹,

Díaz²,

Vicente³

et al. 2020

Parasitology and Microbiology Research

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This study demonstrates the involvement of the cytoskeleton in the movement of cyanobacteria and fungal spores to their hosts to establish a state of symbiosis or pathogenicity. The term symbiosis sensu lato is referred not only to commensalism and mutualism but also to the parasitic aberrations. The establishment of association implies that the endohabitant can move on a wet surface until finding an entry point in the exohabitant surface. In aqueous media, the exohabitant secretes glycoproteins that form a chemoattraction gradient for the invading cells. In lichens, the gradient consists of fungal lectins whose function is to recognize a compatible green alga or cyanobacterium. In the case of pathogens, the secreted proteins usually are a mixture that includes false quorum and chemoattractant signals, and cell wall digestive enzymes. The results indicate that fungal lectins and defense proteins bind to specific cell wall receptors for signaling the activation of cytoskeleton, causing successive cycles of cell contraction-relaxation that permits the migration of the endohabitant. In this study, different biochemical and microscopy techniques have been used. The mechanisms through which the cytoskeleton carries out these cycles of cell contractionrelaxation are described, being this a remarkable advance compared to previous results.

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“…Sugarcane smut is one of the most serious diseases of sugarcane. The disease is caused by the fungus, Sporisorium scitamineum, which infects plants through buds on standing stalks or germinating buds in the soil (Sundar et al, 2012;Sánchez-Elordi et al, 2016), can cause considerable yield losses and reductions in cane quality (Olweny et al, 2008). The best control method is to use resistant cultivars, which are widely available in different countries where sugarcane is grown (Chen et al, 2012), however, costs make this strategy expensive.…”

Section: Introductionmentioning

confidence: 99%

Extracts From Leaves of Allamanda blanchetti Inducing Mechanism of Defense to Diseases in Sugarcane

Oliveira¹,

Almeida²,

Silva³

et al. 2019

JAS

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This research aims to analyze the effect of the extract from a native plant from Caatinga, Allamanda blanchetti, in the induction of resistance mechanisms in a sugarcane variety smut-susceptible. Initially, it carried out a phytochemical analysis to know the main plant compounds used in this study. Diverse chemicals content in ethanolic extract from A. blanchetii were detected by thin-layer chromatography (TLC). Flavonoids, were more abundant compounds following by terpenes, stereroids and saponins. Under greenhouse conditions the sugarcane plants, SP-791011 (smut-susceptible), were sprayed with extracts from A. blanchetti extracted at cold at concentrations of 1000 ppm and acilbenzolar-S-metil (ASM) (100 mg/L). Leaves were collected at 0, 24 and 48 hours after spraying and used in the RT-PCR analysis for to identify the defense gene expression. Change in gene expression were observed in the different treatments, especially in the expression of pathogenesis-related (PR) genes. The extract of A. blanchetti induced an increase in the glucanase expression and was more effective than ASM inducer. SNPR1 gene show increased in the two treatment. The results indicate that A. blanchetti extracts was able to activate the resistance mechanism as observed in resistant plants. This paper is the first report about the use of Caatinga natural plant extracts inducing resistance genes against Sporisorium scitamineum in sugarcane susceptible genotype.

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Defence sugarcane glycoproteins disorganize microtubules and prevent nuclear polarization and germination of Sporisorium scitamineum teliospores

Cited by 12 publications

References 66 publications

Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Extracts From Leaves of Allamanda blanchetti Inducing Mechanism of Defense to Diseases in Sugarcane

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