Defects of Immune Cells in the Senescence-Accelerated Mouse: A Model for Learning and Memory Deficits in the Aged

Abe, Yuko; Yuasa, Manami; Kajiwara, Yoshio; Hosono, Masamichi

doi:10.1006/cimm.1994.1205

Cited by 28 publications

(19 citation statements)

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“…To examine the immune dysfunction, if any, in SAMP8 and SAMP10 mice with special reference to neurodegeneration. Only a few data are available for SAMP8 mice [123,124]. 11.…”

Section: Future Directionsmentioning

confidence: 99%

Senescence-Accelerated Mouse (SAM) with Special References to Neurodegeneration Models, SAMP8 and SAMP10 Mice

Takeda¹

2009

Neurochem Res

215

188

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The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging common to the SAMP and SAMR is accelerated senescence and normal aging, respectively. Furthermore, SAMP and SAMR strains of mice manifest various pathobiological phenotypes spontaneously. Among SAMP strains, SAMP8 and SAMP10 mice show age-related behavioral deterioration such as deficits in learning and memory, emotional disorders (reduced anxiety-like behavior and depressive behavior) and altered circadian rhythm associated with certain pathological, biochemical and pharmacological changes. Here, the previous and recent literature on SAM mice are reviewed with an emphasis on SAMP8 and SAMP10 mice. A spontaneous model like SAM with distinct advantages over the gene-modified model is hoped by investigators to be used more widely as a biogerontological resource to explore the etiopathogenesis of accelerated senescence and neurodegenerative disorders.

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“…To examine the immune dysfunction, if any, in SAMP8 and SAMP10 mice with special reference to neurodegeneration. Only a few data are available for SAMP8 mice [123,124]. 11.…”

Section: Future Directionsmentioning

confidence: 99%

Senescence-Accelerated Mouse (SAM) with Special References to Neurodegeneration Models, SAMP8 and SAMP10 Mice

Takeda¹

2009

Neurochem Res

215

188

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“…Previous studies have demonstrated that decreased T cell immune function is closely related to age-associated cognitive impairment in SAMP8 mice (23-25). The cause of the decreased T cell immune function in SAMP8 mice remains an open question.…”

Section: Resultsmentioning

confidence: 99%

“…Splenocytes were isolated as described by Abe et al (23). RNA from splenocytes was extracted using TRIzol reagents (Invitrogen Corporation, USA) according to manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice

Guo¹,

Qi²,

Zhou³

et al. 2013

Braz J Med Biol Res

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We evaluated changes in levels by comparing serum proteins in senescence-accelerated mouse-prone 8 (SAMP8) mice at 2, 6, 12, and 15 months of age (SAMP8-2 m, -6 m, -12 m, -15 m) to age-matched SAM-resistant 1 (SAMR1) mice. Mice were sacrificed, and blood was analyzed by 2-dimensional electrophoresis combined with mass spectrometry. Five protein spots were present in all SAMP8 serum samples, but only appeared in SAMR1 samples at 15 months of age except for spot 3, which also showed a slight expression in SAMR1-12 m sera. Two proteins decreased in the sera from SAMP8-2 m, -6 m, and -12 m mice, and divided into 2 spots each in SAMP8-15 m sera. Thus, the total number of altered spots in SAMP8 sera was 7; of these, 4 were identified as Ig kappa chain V region (M-T413), chain A of an activity suppressing Fab fragment to cytochrome P450 aromatase (32C2_A), alpha-fetoprotein, and apolipoprotein A-II. M-T413 is a monoclonal CD4 antibody, which inhibits T cell proliferation. We found that M-T413 RNA level was significantly enhanced in splenocytes from SAMP8-2 m mice. This agreed with serum M-T413 protein alterations and a strikingly lower blood CD4+ T cell count in SAMP8 mice when compared to the age-matched SAMR1 mice, with the latter negatively correlating with serum M-T413 protein volume. Age-related changes in serum proteins favored an increase in autoantibodies and alpha-fetoprotein and a decrease of apolipoprotein A-II, which occurred in SAMP8 mice at 2 months of age and onwards. These proteins may serve as candidate biomarkers for early aging.

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“…5 As early as two months after birth, SAMP8 mice show markedly lower activity for the immune indices such as natural killer (NK) cell activity, in vitro anti-sheep red blood cells antibody responses, cell proliferation, and IL-2-production to concanavalin A (Con A) stimulation than their normal counterparts, SAMR1 mice. 6 A study showed that a diet supplemented with nucleoside-nucleotide mixture enhance the immune response in protein-deprived SAMP8 mice. 7 Similarly, dietary supplementation with…”

Section: Introductionmentioning

confidence: 99%

Nanonized black soybean enhances immune response in senescence-accelerated mice

Chan

Wu²,

Chan³

et al. 2009

IJN

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Soy isofl avones may have applications in cancer prevention and anti-infl ammation, therefore this study was conducted to investigate the effect of dietary supplementation with black soybean on the immune response in the senescence-accelerated-prone mice (SAMP8) and -resistant mice (SAMPR1, as controls). The mechanism of isofl avones was also investigated. Six-month-old male SAMP8 and SAMR1 mice were divided into the control groups and experimental groups supplemented with nanonized (Nano-soy) or microparticled (Micro-soy) black soybeans (n = 8/group), respectively for 12 weeks. Human peripheral blood mononuclear cells (PBMC) and murine splenocytes were stimulated with mitogens and cytokines were determined by reverse transcriptase-polymerase chain reaction and/or ELISA. The results showed that body weight, food intake, and relative weights of organs did not differ among the SAMP8 control and experimental groups. Isofl avone (daidzin and genistin) intake was higher in the Nano-soy group than the Micro-soy group. The lymphoproliferation and production of interleukin-2 (IL-2) and interferon-gamma (IFN-γ) in the Nano-soy group had a signifi cantly higher (P Ͻ 0.05) than those in the control and Micro-soy groups. The Nano-soy supplemented mice reached these cytokine levels similar to SAMR1 mice. This result was consistent with the in vitro data that daidzein (a metabolite of daidzin), at a concentration of 10 μM, increased IL-2, IL-4, and IFN-γ production from phytohemagglutinin-stimulated PBMC (P Ͻ 0.05). However at higher concentrations (Ͼ50 μM), daidzein only reduced IL-10 and IFN-γ levels, whereas genistein reduced levels of the IL-2, IL-4, IL-10, IFN-γ mRNA and protein and these results suggest that the Nano-soy supplementation improved immune response in SAMP8 mice which may be attributable to higher daidzin content in the black soybean preparation.

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Defects of Immune Cells in the Senescence-Accelerated Mouse: A Model for Learning and Memory Deficits in the Aged

Cited by 28 publications

References 0 publications

Senescence-Accelerated Mouse (SAM) with Special References to Neurodegeneration Models, SAMP8 and SAMP10 Mice

Senescence-Accelerated Mouse (SAM) with Special References to Neurodegeneration Models, SAMP8 and SAMP10 Mice

Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice

Nanonized black soybean enhances immune response in senescence-accelerated mice

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