Defects in the ratio of the dynein isoform, DHC11 in the long-flagella mutants of Chlamydomonas reinhardtii

“…The results suggested that the anaerobic environment inside of flocs plays an important role in activating H 2 production. Given that the single-step preparation of H 2 -producing microalgal aggregates by flocculants is both fast and inexpensive, we also envisage that with the aid of controllably self-assembling biomaterials, 45 bio-flocculants, 46 and genetically engineered microalgae, 47,48 our approach could be applied to large-scale green H 2 production to help achieve carbon neutrality. 49 ■ EXPERIMENTAL SECTION Materials.…”

Section: ■ Conclusionmentioning

confidence: 99%

Chemical Flocculation-Based Green Algae Materials for Photobiological Hydrogen Production

Chen

et al. 2022

ACS Appl. Bio Mater.

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Photobiological hydrogen production is among the most promising ways toward the mass production of hydrogen energy. The use of green algal aggregates to produce photobiological hydrogen has attracted much attention because it overcomes the limitations of sulfur deprivation and oxygen scavengers. However, the current preparation of green algal aggregates that are capable of hydrogen production is time-consuming and laborious, leading to a difficulty in large-scale applications. Here, we demonstrated that the chemical flocculation of green algae is able to generate aggregates for photobiological hydrogen production. We find that Chlorella pyrenoidosa can directly form aggregates in the original liquid cultures by a commercial chemical flocculant, cationic etherified starch, thereby achieving sustainable hydrogen production for 11 days under continuous light irradiation, and the average rate of photobiological production reaches 0.37 μmol H 2 (mg chlorophyll•h) −1 . This research provides a feasible approach for preparing a low-cost photobiological hydrogen production system helping to realize carbon neutrality.

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Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis

Hou,

Guo,

Liu

et al. 2024

Bioresour. Bioprocess.

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Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.

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Defects in the ratio of the dynein isoform, DHC11 in the long-flagella mutants of Chlamydomonas reinhardtii

Cited by 3 publications

References 33 publications

DRC2/CCDC65 is a central hub for assembly of the nexin–dynein regulatory complex and other regulators of ciliary and flagellar motility

DRC2/CCDC65 is a central hub for assembly of the nexin–dynein regulatory complex and other regulators of ciliary and flagellar motility

Chemical Flocculation-Based Green Algae Materials for Photobiological Hydrogen Production

Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis

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