Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants

Yang, Fen; Fernández-Jiménez, Nadia; Tučková, Martina; Vrána, Jan; Cápal, Petr; Díaz, Mariana; Pradillo, Mónica; Pečinka, Aleš

doi:10.1093/plcell/koab178

Cited by 18 publications

(30 citation statements)

References 58 publications

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“…Data related to diploid controls were published in a separate study (Yang et al, 2021). Both diploid and tetraploid plants were cultivated under the same growth conditions.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Tetraploidy Enhances Fertility Defects In Nse2-1 and Nse4a-2 Plantsmentioning

confidence: 99%

“…The diploid (2x) Arabidopsis nse2 mutant plants have a significantly reduced fertility (Liu et al, 2014;Yang et al, 2021). To analyze the dosage-dependent role of NSE2, we produced autotetraploid wild-type (4x WT) and nse2-1 (4x nse2-1) plants (Yang et al, 2021). With these lines, we noticed that 4x nse2-1 plants had a reduced root length in the juvenile stage and a lower plant height at the adult stage compared with 4x WT (Figures 1A,B).…”

Section: Tetraploidy Enhances Fertility Defects In Nse2-1 and Nse4a-2 Plantsmentioning

confidence: 99%

“…NSE2 function is not essential for Arabidopsis survival, but the plants are strongly affected in their vegetative and generative development including poor growth of roots, earlier flowering, reduced height, and decreased fertility (Huang et al, 2009;Ishida et al, 2009;Xu et al, 2013;Liu et al, 2014;Kwak et al, 2016). Recently, several studies pointed toward the importance of the SMC5/6 complex during plant sexual reproduction, including meiosis, pollen viability, and seed development (Liu et al, 2014;Díaz et al, 2019;Yang et al, 2021;Zou et al, 2021). NSE2, NSE4, and SNI1 were found to play an important role in meiosis.…”

Section: Introductionmentioning

confidence: 99%

“…Here, RAD51 directly suppresses the SMC5/6 complex to promote DMC1-based recombination (Chen et al, 2021). Another role of the SMC5/6 complex is to secure the development of properly reduced haploid gametes in meiotic recombination independent manner (Yang et al, 2021). The NSE2, NSE4A, and SNI1 mutants show recombination-independent problems in chromosome segregation and produce unreduced microspores.…”

Section: Introductionmentioning

confidence: 99%

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Structural Maintenance of Chromosomes 5/6 Complex Is Necessary for Tetraploid Genome Stability in Arabidopsis thaliana

et al. 2021

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Polyploidization is a common phenomenon in the evolution of flowering plants. However, only a few genes controlling polyploid genome stability, fitness, and reproductive success are known. Here, we studied the effects of loss-of-function mutations in NSE2 and NSE4A subunits of the Structural Maintenance of Chromosomes 5/6 (SMC5/6) complex in autotetraploid Arabidopsis thaliana plants. The diploid nse2 and nse4a plants show partially reduced fertility and produce about 10% triploid offspring with two paternal and one maternal genome copies. In contrast, the autotetraploid nse2 and nse4a plants were almost sterile and produced hexaploid and aneuploid progeny with the extra genome copies or chromosomes coming from both parents. In addition, tetraploid mutants had more severe meiotic defects, possibly due to the presence of four homologous chromosomes instead of two. Overall, our study suggests that the SMC5/6 complex is an important player in the maintenance of tetraploid genome stability and that autotetraploid Arabidopsis plants have a generally higher frequency of but also higher tolerance for aneuploidy compared to diploids.

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“…Data related to diploid controls were published in a separate study (Yang et al, 2021). Both diploid and tetraploid plants were cultivated under the same growth conditions.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Tetraploidy Enhances Fertility Defects In Nse2-1 and Nse4a-2 Plantsmentioning

confidence: 99%

Section: Tetraploidy Enhances Fertility Defects In Nse2-1 and Nse4a-2 Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structural Maintenance of Chromosomes 5/6 Complex Is Necessary for Tetraploid Genome Stability in Arabidopsis thaliana

et al. 2021

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The structural maintenance of chromosomes 5 is a possible biomarker for individualized treatment of colorectal cancer

et al. 2022

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Background Although the understanding of resistance to oxaliplatin (OXA) chemotherapy in colorectal cancer (CRC) has been sought for many years, drug tolerance remains a major challenge for cancer therapy. Revealing the molecular mechanism of OXA resistance could help to explain the poor prognosis of patients. Methods Gene expression omnibus (GEO) database was searched, GSE83129, which contains RNA profiling in metastatic CRC patients treated first‐line with OXA, was chosen for the following analysis. Differential expressed genes (DEGs) between the adenocarcinoma and adjacent_normal team, respectively, in the OXA responders and no‐responders were analyzed. The Gene Ontology (GO) and hub genes in the protein–protein interaction (PPI) network were used for the molecular mechanism of OXA resistance. Tumor‐related databases were used for the clinical relevance of the structural maintenance of chromosomes 5 ( SMC5 ) in CRC. The in vitro assays were used to detect the molecular function of SMC5 in CRC cells. Quantitative real‐time PCR (qRT‐PCR) and western blot were used to detect the expression of the structural maintenance of chromosomes 5/6 (SMC5/6) complex components upon OXA and raltitrexed (RTX) treatment. CCK‐8 was used to detect the cell viability of cells with different treatment. Results SMC5 was downregulated in CRC tissues of OXA no‐response patients. Lower expression of SMC5 was correlated with a poor prognosis in CRC patients, improved this gene expression, inhibited the CRC cell growth and invasion in vitro. Furthermore, SMC5 was downregulated upon OXA treatment in CRC cells, while RTX would reverse its expression, and the combination of these two drugs restored the SMC5 level to the normal situation. Finally, RTX treatment enhanced the OXA cytotoxicity. Conclusion SMC5 is a tumor suppressor, that low expression of this gene is benefit for the development of CRC. Combination treatment with RTX and OXA may be more suitable for those OXA no‐responders with lower SMC5 .

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TaLBD1, a LOB transcription factor gene in T. aestivum, confers plant adaptation to low-N stress via modulating N acquisition-associated processes

Zhang

et al. 2022

Plant Cell Tiss Organ Cult

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Distinct members of the transcription factor (TF) families act as the critical mediators in plant low-N stress response by regulating transcription of the stress defensive-associated genes. In this study, we characterized TaLBD1, a member of the Lateral Organ Boundary (LOB) TF family in T. aestivum, in modulating plant adaptation to the N deprivation condition. The TaLBD1 protein harbors conserved domains speci ed by the plant LOB TF proteins, which targeted onto nucleus after sorted from endoplasmic reticulum (ER). The transcripts of TaLBD1 in roots and aerial tissue were shown to be response to modi ed external N levels, showing in the tissues with a pattern to be gradually upregulated following a 27-h N starvation treatment. Transgene characterization on TaLBD1 in N. tabacum indicated that it confers plants improved phenotype, root system architecture (RSA) establishment, dry mass production, and N accumulation under N starvation (NS) condition. Analyses on expression patterns of the genes in nitrate transporter (NRT) and those in PIN-FORMED (PIN) families revealed that the NRT gene NtRNT2.4 and PIN gene NtPIN6 displayed signi cantly upregulated expression in the NS-challenged lines. Further knockdown expression of them indicated that NtNRT2.4 regulates N uptake whereas NtPIN6 modulates RSA behavior. These results suggested that the TaLBD1-mediated plant NS adaptation is associated with its role in transcriptionally regulating distinct NRT and PIN members that improve N uptake and RSA establishment of plants upon low-N stress. High throughput RNA-seq analyses indicated that a quantity of genes modi ed expression upon NS underlying TaLBD1 regulation. These differentially expressed (DE) genes were enriched into the gene ontology (GO) terms associated with signal transduction, transcription, protein biosynthesis, primary or secondary metabolism, and stress defensiveness. Our results together suggested that TaLBD1 acts as one of the crucial regulators in plant adaptation to low-N stress. It can be used as a valuable index for evaluating plant NS response and a potential target for molecular breeding of high N use e ciency (NUE) crop cultivars. Key MessageThe member of the LOB transcription factor family TaLBD1 in T. aestivum confers plant tolerance to N starvation stress via the role in modulating N uptake and RSA establishment.

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Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants

Cited by 18 publications

References 58 publications

Structural Maintenance of Chromosomes 5/6 Complex Is Necessary for Tetraploid Genome Stability in Arabidopsis thaliana

Structural Maintenance of Chromosomes 5/6 Complex Is Necessary for Tetraploid Genome Stability in Arabidopsis thaliana

The structural maintenance of chromosomes 5 is a possible biomarker for individualized treatment of colorectal cancer

TaLBD1, a LOB transcription factor gene in T. aestivum, confers plant adaptation to low-N stress via modulating N acquisition-associated processes

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