Eâcigarettes are perceived as harmless; however, evidence of their safety is lacking. New data suggests Eâcigarettes discharge a range of compounds capable of physiological damage to users. We previously established that cigarette smoke caused defective alveolar macrophage phagocytosis. The present study compared the effect Eâcigarette of components; Eâliquid flavors, nicotine, vegetable glycerine, and propylene glycol on phagocytosis, proinflammatory cytokine secretion, and phagocytic recognition molecule expression using differentiated THPâ1 macrophages. Similar to CSE, phagocytosis of NTHi bacteria was significantly decreased by Eâliquid flavoring (11.65â15.75%) versus control (27.01%). Nicotine also decreased phagocytosis (15.26%). Eâliquid, nicotine, and Eâliquid+ nicotine reduced phagocytic recognition molecules; SRâA1 and TLRâ2. ILâ8 secretion increased with flavor and nicotine, while TNF
α, ILâ1ÎČ, ILâ6, MIPâ1α, MIPâ1ÎČ, and MCPâ1 decreased after exposure to most flavors and nicotine. PG, VG, or PG:VG mix also induced a decrease in MIPâ1α and MIPâ1ÎČ. We conclude that Eâcigarettes can cause macrophage phagocytic dysfunction, expression of phagocytic recognition receptors and cytokine secretion pathways. As such, Eâcigarettes should be treated with caution by users, especially those who are nonsmokers.