Defective in vitro contractility of ureteropelvic junction in children with functional and obstructive urine flow impairment

Pontincasa, Piero; Bartoli, Fabio; Ciaula, Agostino Di; Minerva, Francesco; Niglio, Francesco; Gentile, Ottavio; Leggio, Samuele; Leggio, A; Palasciano, Giuseppe

doi:10.1016/j.jpedsurg.2006.05.049

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…This was the first study designed to examine the protein variations and contents of the obstructed segment in comparison to distal and proximal segments. Previous studies focused on the selected known proteins and/or structures of the smooth muscle, extracellular matrix, or neural ingredients and thus produced limited information [Portincasa et al, 2006;Kaya et al, 2010;Özel et al, 2010;Tourchi et al, 2014]. We found several protein expression differences to discuss among the mentioned tissue sections.…”

Section: Discussionmentioning

confidence: 93%

“…For example, in one study, changes in smooth muscle associated with disease etiology were emphasized [Hosgor et al, 2005], while in another study muscle density was reported to be higher in cases with external obstructions than in cases with intrinsic obstruction [Yiee et al, 2010]. In addition, changes at the various extracellular matrix proteins were observed, along with depleted nerve supply and/or dissolved smooth muscular coat in the obstructed segment [Portincasa et al, 2006;Kaya et al, 2010;Özel et al, 2010;Tourchi et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue

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et al. 2020

Cells Tissues Organs

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Ureteropelvic junction (UPJ) obstruction is a common problem in children, but its etiology remains unclear. In this study, the proteome profiles of the obstructed segment and its surrounding distal and proximal parts were comparatively evaluated. Twelve children younger than 2 years of age with unilateral intrinsic UPJ obstruction were included. The excised operational tissue was divided into three parts immediately after resection: the obstructed part (Obst), the distal normal ureteral part (Dist), and the proximal part of the obstructed segment (Prox). Proteins extracted from the tissue samples were subjected to two-dimensional gel electrophoresis analysis to identify differentially regulated proteins. Spot analysis revealed that four proteins, namely tropomyosin beta and alpha-1 chains, actin and desmin, were upregulated in Obst in comparison to Dist. A similar analysis between Obst and Prox showed that heat shock protein beta-1 and carbonic anhydrase-1 were upregulated in Obst, while tropomyosin alpha 3 chain and ATP synthase beta were upregulated in Prox. The last comparative analysis between Dist and Prox revealed upregulation of annexin-A5 and annexin-A1 in Dist and vimentin, mitochondrial ATP synthase subunit-beta, peroxiredoxin-2, and apolipoprotein-A1 in Prox. Bioinformatics analysis using the STRING server indicated that the differentially regulated proteins, altogether, point to the changes occurring in muscle filament sliding pathway. When regulations occurring in each group were mutually compared, a change in lipase inhibition activity was detected by STRING. This is the first study scrutinizing changes occurring in protein profiles in UPJ.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue

Görmüş

Kasap

Akpınar

et al. 2020

Cells Tissues Organs

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“…Its diagnosis and treatment are still controversial, and diuretic renography seems to be the best way to discriminate obstructive from functional UPJO. Portincasa et al [1] have demonstrated significant deterioration of smooth muscle cell contractility at the ureteropelvic junction in both obstructive and functional UPJO, and hence, transition from mild to severe obstruction caused by smooth muscle cell dysfunction in the stenotic segment can be observed.…”

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confidence: 99%

Urinary epidermal growth factor, monocyte chemotactic protein-1, and β2-microglobulin in children with ureteropelvic junction obstruction

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Aceto

et al. 2011

Journal of Pediatric Surgery

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“…16 The study conducted by Portincasa et al on this subject has shown that there is a significant deterioration in the smooth muscle contraction at the ureteropelvic junction in both obstructive and functional UPO cases. 17 USG and scintigraphy are major screening methods in diagnosing UPO, evaluating the patients after surgery, and monitoring patients who have not undergone a surgical operation yet. 18,19 Biochemical indicators such as TGF-b 1 and b 2 microglobulin, urine, and blood electrolytes are also utilized in diagnosing and monitoring UPO in the recent years.…”

Section: Discussionmentioning

confidence: 99%

The effect of alfuzosin on renal resistive index, urinary electrolytes and β2 microglobulin levels and TGF β-1 levels of kidney tissue in rats with unilateral ureteropelvic junction obstruction

et al. 2016

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(2016) The effect of alfuzosin on renal resistive index, urinary electrolytes and β2 microglobulin levels and TGF β-1 levels of kidney tissue in rats with unilateral ureteropelvic junction obstruction, Renal Failure, Background: In this study, it was aimed to determine the effects of alfuzosin on experimentally generated unilateral partial ureteropelvic junction obstruction (UPO) in rats. Materials and methods: Thirty Long-Evans rats were randomly allocated into five groups. In control group (C), nothing was performed; in group Sham (S) only laparotomy was done; in Alfuzosin group (A) only alfuzosin was administered for two weeks (10 mg/kg/day p.o.) without any surgery; in UPO group, unilateral UP junction obstruction was produced; and in the Group UPT (ureteropelvic obstruction þ treatment), alfuzosin was administered for two weeks (10 mg/kg/day p.o.) in addition to UPO production. Renal pelvic anteroposterior diameters were determined with ultrasonography (USG) and renal arterial resistivity indexes by color Doppler USG. Urine was collected both at the beginning and at the end of the experiment for 24 h in all the groups and at the end of the experiment, blood samples were obtained. Blood and urine electrolytes and TGFb1, urine density, urine b 2 microglobulin levels were determined. Renal tissue samples harvested from all of the rats were histopathologically evaluated. Results were determined using one-way ANOVA t-test; p < 0.05 was accepted as significant. Results: Urine density in the UPT group was lower with respect to UPO group and blood electrolytes were preserved as close to normal (p < 0.05). In the UPT group, urine TGF-b1 and blood TGFb1, blood b 2 microglobulin levels and histopathologic damage scores were lower compared to the UPO group (p < 0.05). Conclusion: It is shown in this experimental unilateral partial UPO model that alfuzosin treatment prevents obstructive renal damage.

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Defective in vitro contractility of ureteropelvic junction in children with functional and obstructive urine flow impairment

Cited by 7 publications

References 15 publications

Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue

Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue

Urinary epidermal growth factor, monocyte chemotactic protein-1, and β2-microglobulin in children with ureteropelvic junction obstruction

The effect of alfuzosin on renal resistive index, urinary electrolytes and β2 microglobulin levels and TGF β-1 levels of kidney tissue in rats with unilateral ureteropelvic junction obstruction

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