Defective CD19-dependent signaling in B-1a and B-1b B lymphocyte subpopulations

Sen, Goutam; Wu, Ho‐Ting; Bikah, Gabriel; Venkataraman, Chandrasekhar; Robertson, Darrell A.; Snow, E Charles; Bondada, Subbarao

doi:10.1016/s0161-5890(02)00047-0

Cited by 28 publications

(38 citation statements)

References 50 publications

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“…Intracellular calcium levels were measured by methods described previously (31). Thymocytes were loaded with 1 µM Indo-1 AM per 10 × 10 6 cells for 30 min at 37°C.…”

Section: Calcium Release Analysismentioning

confidence: 99%

CD5 plays an inhibitory role in the suppressive function of murine CD4+ CD25+ Treg cells

et al. 2008

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A subset of CD4 + T cells, the CD4 + CD25 + T reg cells in the lymphoid organs and peripheral blood are known to possess suppressive function. Previous in vitro and in vivo studies have indicated that T cell receptor (TCR) signal is required for development of such 'natural regulatory (T reg ) cells' and for activation of the effector function of CD4 + CD25 + regulatory T cells. CD5 is a cell surface molecule present on all T cells and a subtype of B lymphocytes, the B-1 cells, primarily localized to coelomic cavities, Peyer's patches, tonsils and spleen. CD5 acts as a negative regulator of T cell and B cell signaling via recruitment of SHP-1. Here, we demonstrate that T reg cells obtained from CD5 −/− mice are more potent than those from wild type mice in suppressing the in vitro cell proliferation of anti-CD3 stimulated CD4 + CD25 − responder T cells. This phenomenon was cell contact and GITR dependent. Lack of CD5 expression on T reg cells (from spleen, lymph node and thymus) did not affect the intracellular levels of Foxp3. However, CD5 −/− T reg thymocytes were able to elicit a higher Ca 2+ response to TCR + co-stimulatory signals than the wild type cells. CD5 −/− mice expressed more Foxp3 mRNA in the colon than wild type mice, and additionally, the severity of the DSS-induced colitis in CD5 −/− mice was less than the wild type strain. We suggest that manipulation of CD5 expression or the downstream signaling components of CD4 + CD25 + T reg cells as a potential strategy for therapeutic intervention in cases of auto-immune disorders.

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“…Intracellular calcium levels were measured by methods described previously (31). Thymocytes were loaded with 1 µM Indo-1 AM per 10 × 10 6 cells for 30 min at 37°C.…”

Section: Calcium Release Analysismentioning

confidence: 99%

CD5 plays an inhibitory role in the suppressive function of murine CD4+ CD25+ Treg cells

et al. 2008

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“…It has been shown that Ag receptor stimulation is not sufficient to induce activation of B-1 cells, because CD5 that is expressed on B-1a cells, but not on B-2 cells, negatively regulates IgM receptor-mediated signaling in B-1 cells (21,22). In addition, non-CD5-mediated, negative regulation of IgM receptor signaling has been reported (23).…”

Section: Role Of Chemokine Receptors In Migration Of Cs-initiating B-mentioning

confidence: 99%

An Hour after Immunization Peritoneal B-1 Cells Are Activated to Migrate to Lymphoid Organs Where within 1 Day They Produce IgM Antibodies That Initiate Elicitation of Contact Sensitivity

Itakura

Szczepanik

Campos

et al. 2005

The Journal of Immunology

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Elicitation of contact sensitivity (CS), a classic example of T cell-mediated immunity, requires Ag-specific IgM Abs to trigger an initiation process. This early process leads to local recruitment of CS-effector T cells after secondary Ag challenge. These Abs are produced by the B-1 subset of B cells within 1 day after primary skin immunization. In this study we report the surprising observation that B-1 cells in the peritoneal cavity are activated as early as 1 h after naive mice are painted with a contact-sensitizing Ag on the skin of the trunk and feet to begin the initiation of CS. B-1 cells in the spleen and draining lymph nodes produce the initiating Abs by 1 day after immunization, when we found increased numbers of Ag-specific IgM Ab-producing cells in these tissues by ELISPOT assay. Importantly, we show that contact-activated peritoneal B-1 cells migrate to these lymphoid tissues and then differentiate into Ag-specific IgM Ab-producing cells, resulting in specific CS-initiating IgM Abs in the serum by 1 day. Furthermore, pertussis toxin, which is known to inhibit signaling via G protein-coupled chemokines, inhibited the migration of contact-activated peritoneal B-1 cells to the lymphoid tissues, probably due to BLR-1 (Burkitt lymphoma receptor-1). These findings indicate that within 1 h after contact skin immunization, B-1 cells in the peritoneal cavity are activated to migrate to the lymphoid tissues by chemokine-dependent mechanisms to produce serum Ag-specific IgM Abs within 1 day after immunization, leading to local recruitment of CS-effector T cells.

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“…Cell transfer studies then showed that these populations, also referred to as subsets, are developmentally distinct, in that, each is capable of fully replenishing its niche when sorted and transferred to adoptive recipients and neither has the capability to more than minimally replenish the other (2,5,6). Consistent with this developmental distinction, and with differences noted between the B-1a and B-1b repertoire, recent studies have shown that B-1a and B-1b have distinct immune functions and respond differently to antigenic stimulation (1,(7)(8)(9)(10)(11)(12).…”

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confidence: 94%