Defective ATP‐dependent mucin secretion by cystic fibrosis pancreatic epithelial cells

Montserrat, Colette; Merten, Marc; Figarella, Catherine

doi:10.1016/0014-5793(96)00900-3

Cited by 21 publications

(13 citation statements)

References 36 publications

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“…CFTR in intracellular compartments of goblet cells could function on the biosynthetic pathway (39) in constitutive membrane trafficking (40) and in coupling secretion of mucus, water, and salt (41,42). A role for CFTR in cAMP-and chloride-dependent exocytosis and endocytosis was demonstrated for the wild-type, but not for a defective, chloride channel (43) and may be attributed to an association of the vesicle fusion process with electrolyte transport (44).…”

Section: Discussionmentioning

confidence: 99%

ΔF508 CFTR protein expression in tissues from patients with cystic fibrosis

Kälin¹,

Claass²,

Sommer³

et al. 1999

J. Clin. Invest.

249

179

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Section: Discussionmentioning

confidence: 99%

ΔF508 CFTR protein expression in tissues from patients with cystic fibrosis

Kälin¹,

Claass²,

Sommer³

et al. 1999

J. Clin. Invest.

249

179

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“…ATP induced mucin secretion in cell lines expressing functional CFTR: Capan-1 cells, CFTR-corrected CFPAC-1 cells, but not in CFPAC-1 cells [129]. It seems that receptors other than P2Y 2 (or P2Y 4 ) are involved in mucin secretion as the sequence of efficacy was different to that for ion transport, i.e.…”

Section: Pancreatic Acinimentioning

confidence: 96%

Purinergic receptors in the endocrine and exocrine pancreas

Novak

2007

Purinergic Signalling

115

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The pancreas is a complex gland performing both endocrine and exocrine functions. In recent years there has been increasing evidence that both endocrine and exocrine cells possess purinergic receptors, which influence processes such as insulin secretion and epithelial ion transport. Most commonly, these processes have been viewed separately. In β cells, stimulation of P2Y 1 receptors amplifies secretion of insulin in the presence of glucose. Nucleotides released from secretory granules could also contribute to autocrine/paracrine regulation in pancreatic islets. In addition to P2Y 1 receptors, there is also evidence for other P2 and adenosine receptors in β cells (P2Y 2 , P2Y 4 , P2Y 6 , P2X subtypes and A 1 receptors) and in glucagon-secreting α cells (P2X 7 , A 2 receptors). In the exocrine pancreas, acini release ATP and ATP-hydrolysing and ATP-generating enzymes. P2 receptors are prominent in pancreatic ducts, and several studies indicate that P2Y 2 , P2Y 4 , P2Y 11 , P2X 4 and P2X 7 receptors could regulate secretion, primarily by affecting Cl − and K + channels and intracellular Ca 2+ signalling. In order to understand the physiology of the whole organ, it is necessary to consider the full complement of purinergic receptors on different cells as well as the structural and functional relation between various cells within the whole organ. In addition to the possible physiological function of purinergic receptors, this review analyses whether the receptors could be potential therapeutic targets for drug design aimed at treatment of pancreatic diseases.

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“…These results are in line with the preceding data of Lloyd Mills et al (6) on these same cells, showing that the incorporation of anti-CFTR antibodies into the cells inhibited ␤-adrenergic-stimulated mucin secretion (6). Moreover, it has been shown that the transfection of cDNA for wild type CFTR into CFPAC-1 cells, which conferred cAMP-dependent regulation of a Cl Ϫ conductance (47), restored the defective ATP-induced mucin secretion observed in CF cells (48). Similarly, adenovirus-mediated gene transfer of CFTR to immortalized CF human tracheal epithelial cells restored defective cAMP-dependent secretion not only of chloride but also of glycoconjugates (49).…”

Section: Mpb Drugs Stimulate the Antibacterial Function Of Human Tracmentioning

confidence: 99%