Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase–deficient Mice but not in Mast Cell–deficient Mice

Ghosh, Asok; Hirasawa, Noriyasu; Ohtsu, Hiroshi; Watanabe, Takehiko; Ohuchi, Kazuo

doi:10.1084/jem.20011782

Cited by 107 publications

(101 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…sized by HDC induced in infiltrating macrophages, and VEGF, a factor essential for new blood vessel formation. Granulation and angiogenesis are impaired in HDC-deficient mice, in which induction of VEGF in the granulation tissues is also markedly reduced (25). This model of inflammation also depends on prostanoids (45), a notion also supported by our present study showing an inhibitory effect of indomethacin (Fig.…”

Section: Discussionsupporting

confidence: 85%

“…Subcutaneous implantation of a cotton thread into the dorsum of the mouse induces formation of granulation tissue associated with angiogenesis (25). In this model, the process of inflammation depends on histamine, which is de novo synthe- FIG.…”

Section: Discussionmentioning

confidence: 99%

“…Cotton Thread-induced Granulation Tissue Formation-Cotton threads (Araiwa Co.) were washed overnight with ethyl acetate and dried at room temperature before being cut into 1-cm lengths (3 mg weight), and sterilized by dry heat at 180°C for 2 h. The cotton threads were implanted subcutaneously into the dorsum of anesthetized mice by using a 13-gauge implant needle (Natume), as described previously (25). After appropriate periods, the mice were anesthetized and killed, and the granulation tissues were dissected together with the cotton threads and weighed.…”

Section: Methodsmentioning

confidence: 99%

“…Participation of mPGES-1 in Inflammatory Granulation and Angiogenesis-Subcutaneous implantation of a cotton thread in the dorsum of mice induces formation of granulation tissue with angiogenesis (25). As shown in Fig.…”

Section: Fig 2 Mpges-1 Is An Essential Component For Pge 2 Productimentioning

confidence: 96%

“…Granulation tissue formation and angiogenesis observed in this model depend on the up-regulation of VEGF, an essential angiogenic factor, and HDC, an enzyme required for the synthesis of histamine (25). We therefore assessed by immunoblotting the expression of VEGF and HDC in the granulation tissue of mPGES-1 KO and WT mice.…”

Section: Fig 2 Mpges-1 Is An Essential Component For Pge 2 Productimentioning

confidence: 99%

See 4 more Smart Citations

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Kamei

Yamakawa

Takegoshi

et al. 2004

Journal of Biological Chemistry

Self Cite

264

245

View full text Add to dashboard Cite

We examined the in vivo role of membrane-bound prostaglandin E synthase (mPGES)-1, a terminal enzyme in the PGE 2 -biosynthetic pathway, using mPGES-1 knockout (KO) mice. Comparison of PGES activity in the membrane fraction of tissues from mPGES-1 KO and wild-type (WT) mice indicated that mPGES-1 accounted for the majority of lipopolysaccharide (LPS)-inducible PGES in WT mice. LPS-stimulated production of PGE 2 , but not other PGs, was impaired markedly in mPGES-1-null macrophages, although a low level of cyclooxygenase-2-dependent PGE 2 production still remained. Pain nociception, as assessed by the acetic acid writhing response, was reduced significantly in KO mice relative to WT mice. This phenotype was particularly evident when these mice were primed with LPS, where the stretching behavior and the peritoneal PGE 2 level of KO mice were far less than those of WT mice. Formation of inflammatory granulation tissue and attendant angiogenesis in the dorsum induced by subcutaneous implantation of a cotton thread were reduced significantly in KO mice compared with WT mice. Moreover, collagen antibody-induced arthritis, a model for human rheumatoid arthritis, was milder in KO mice than in WT mice. Collectively, our present results provide unequivocal evidence that mPGES-1 contributes to the formation of PGE 2 involved in pain hypersensitivity and inflammation.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Fig 2 Mpges-1 Is An Essential Component For Pge 2 Productimentioning

confidence: 96%

Section: Fig 2 Mpges-1 Is An Essential Component For Pge 2 Productimentioning

confidence: 99%

See 3 more Smart Citations

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Kamei

Yamakawa

Takegoshi

et al. 2004

Journal of Biological Chemistry

Self Cite

264

245

View full text Add to dashboard Cite

show abstract

Serum Metabolic Fingerprints Characterize Systemic Lupus Erythematosus

Li,

Ding,

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Metabolic fingerprints in serum characterize diverse diseases for diagnostics and biomarker discovery. The identification of systemic lupus erythematosus (SLE) by serum metabolic fingerprints (SMFs) will facilitate precision medicine in SLE in an early and designed manner. Here, a discovery cohort of 731 individuals including 357 SLE patients and 374 healthy controls (HCs), and a validation cohort of 184 individuals (SLE/HC, 91/93) are constructed. Each SMF is directly recorded by nano‐assisted laser desorption/ionization mass spectrometry (LDI MS) within 1 minute using 1 µL of native serum, which contains 908 mass to charge features. Sparse learning of SMFs achieves the SLE identification with sensitivity/specificity and area‐under‐the‐curve (AUC) up to 86.0%/92.0% and 0.950 for the discovery cohort. For the independent validation cohort, it exhibits no performance loss by affording the sensitivity/specificity and AUC of 89.0%/100.0% and 0.992. Notably, a metabolic biomarker panel is screened out from the SMFs, demonstrating the unique metabolic pattern of SLE patients different from both HCs and rheumatoid arthritis patients. In conclusion, SMFs characterize SLE by revealing its unique metabolic pattern. Different regulation of small molecule metabolites contributes to the precise diagnosis of autoimmune disease and further exploration of the pathogenic mechanisms.

show abstract

Combinatorial roles for histamine H₁‐H₂ and H₃‐H₄ receptors in autoimmune inflammatory disease of the central nervous system

et al. 2012

View full text Add to dashboard Cite

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system in which histamine (HA) and its receptors have been implicated in disease pathogenesis. HA exerts its effects through four different G protein-coupled receptors designated H1–H4. We previously examined the effects of traditional single HA receptor (HR) knockouts (KOs) in experimental allergic encephalomyelitis (EAE), the autoimmune model of MS. Our results revealed that H1R and H2R are propathogenic, while H3R and H4R are antipathogenic. This suggests that combinatorial targeting of HRs may be an effective disease-modifying therapy (DMT) in MS. To test this hypothesis, we generated H1H2RKO and H3H4RKO mice and studied them for susceptibility to EAE. Compared with wild-type (WT) mice, H1H2RKO mice developed a less severe clinical disease course, whereas the disease course of H3H4RKO mice was more severe. H1H2RKO mice also developed less neuropathology and disrupted blood brain barrier permeability compared with WT and H3H4RKO mice. Additionally, splenocytes from immunized H1H2RKO mice produced less interferon(IFN)-γ and interleukin(IL)-17. These findings support the concept that combined pharmacological targeting of HRs may be an appropriate ancillary DMT in MS and other immunopathologic diseases.

show abstract

Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase–deficient Mice but not in Mast Cell–deficient Mice

Cited by 107 publications

References 34 publications

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Serum Metabolic Fingerprints Characterize Systemic Lupus Erythematosus

Combinatorial roles for histamine H₁‐H₂ and H₃‐H₄ receptors in autoimmune inflammatory disease of the central nervous system

Contact Info

Product

Resources

About

Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase–deficient Mice but not in Mast Cell–deficient Mice

Cited by 107 publications

References 34 publications

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1

Serum Metabolic Fingerprints Characterize Systemic Lupus Erythematosus

Combinatorial roles for histamine H1‐H2 and H3‐H4 receptors in autoimmune inflammatory disease of the central nervous system

Contact Info

Product

Resources

About

Combinatorial roles for histamine H₁‐H₂ and H₃‐H₄ receptors in autoimmune inflammatory disease of the central nervous system