Defatted bovine milk fat globule membrane inhibits association of enterohaemorrhagic Escherichia coli O157:H7 with human HT-29 cells

Ross, Sarah A.; Lane, Jonathan A.; Kilcoyne, Michelle; Joshi, Lokesh; Hickey, Rita M.

doi:10.1016/j.idairyj.2016.03.001

Cited by 24 publications

(22 citation statements)

References 59 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, O157:H7 EHEC strains appears to express more adhesion factors than O26:H11 strains ( Low et al, 2006 ; Bardiau et al, 2009 ).Thus, for a given EHEC concentration, the presence of more adhesion appendices at the surface of EHEC O157:H7 strain EDL933 might allow it to attach to MFGM more easily than the O26:H11 strain 21765. Glycoconjugates on the fat-aqueous interface of the MFGs presents mimetic analogs of cell receptors involved in recognition of EHEC strains ( Ross et al, 2016 ; Douellou et al, 2017 ). Nevertheless, there were always EHEC cells in the raw milk skimmed phase, whatever the initial EHEC concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Our study report that native MFGs may limit the adhesion of two EHEC strains from serotypes O157:H7 and O26:H11 to Caco-2/HT29-MTX (9:1) intestinal cells in a co-cultured monolayer. EHEC adhesion to intestinal cells cultured in vitro suggests that the integrity of the MFGM-linked glycoconjugates is a necessary condition for inhibiting EHEC adhesion to cells ( Brewster and Paul, 2016 ; Ross et al, 2016 ). The anti-adhesive properties of MFGs depend on fat structure and integrity.…”

Section: Discussionmentioning

confidence: 99%

“…MUC1 is the most efficient milk fat globule membrane (MFGM)-associated glycoprotein for inhibiting bacterial adhesion ( Wang et al, 2001 ; Sprong et al, 2002 ), even if other MFGM-glycoconjugate fractions are also implicated ( Struijs et al, 2013 ). Previous work on MFGM inhibition of pathogens adhesion, including E. coli and Salmonella , demonstrated the effectiveness of these glycoconjugates to limit bacterial colonization ( Guri et al, 2012 ; Ross et al, 2016 ). However, to the best of our knowledge, the impact of MFG on EHEC adhesion in vivo has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Milk Fat Globules Hamper Adhesion of Enterohemorrhagic Escherichia coli to Enterocytes: In Vitro and in Vivo Evidence

et al. 2018

View full text Add to dashboard Cite

Enterohemorrhagic Escherichia coli (EHEC; E. coli) are food-borne agents associated with gastroenteritis, enterocolitis, bloody diarrhea and the hemolytic-uremic syndrome (HUS). Bovine milk glycans have been shown to contain oligosaccharides which are similar to host epithelial cell receptors and can therefore prevent bacterial adhesion. This study aimed to describe interactions between EHEC O157:H7 EDL933 and O26:H11 21765 and milk fat globules (MFGs) in raw milk and raw milk cheese, and the impact of MFGs on EHEC strains adhesion to the intestinal tract in vitro and in vivo. Both EHEC serotypes clearly associated with native bovine MFGs and significantly limited their adhesion to a co-culture of intestinal cells. The presence of MFGs in raw milk cheese had two effects on the adhesion of both EHEC serotypes to the intestinal tracts of streptomycin-treated mice. First, it delayed and reduced EHEC excretion in mouse feces for both strains. Second, the prime implantation site for both EHEC strains was 6 cm more proximal in the intestinal tracts of mice fed with contaminated cheese containing less than 5% of fat than in those fed with contaminated cheese containing 40% of fat. Feeding mice with 40% fat cheese reduced the intestinal surface contaminated with EHEC and may therefore decrease severity of illness.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Milk Fat Globules Hamper Adhesion of Enterohemorrhagic Escherichia coli to Enterocytes: In Vitro and in Vivo Evidence

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The preventive effect of an MFGM supplement against infection with acute otitis media was reported in young children (2 months old) fed infant formula (Timby et al 2015). The ability of defatted bovine MFGM fraction, rich in proteins and glycoproteins, to prevent the association of several enterohaemorrhagic Escherichia coli O157:H7 strains to human colonic adenocarcinoma HT-29 cells was demonstrated in a concentration-dependent and strain-specific manner (Ross et al 2016). Healthy adults were randomly assigned in a placebo-controlled, double-blind, 4 week parallel-intervention study to a milk protein concentrate rich in MFGM or a control diet for 2 weeks and then challenged with an attenuated diarrhoeagenic E. coli strain (Ten Bruggencate et al 2016).…”

Section: Infections and Gut Integritymentioning

confidence: 99%

Milk fat globule membrane: An overview with particular emphasis on its nutritional and health benefits

El-Salam

El-Shibiny

2020

Int J of Dairy Tech

View full text Add to dashboard Cite

Milk fat globule membrane (MFGM) is a minor milk constituent composed of a large number of polar lipid (PL) classes and proteins organised in a tri-layer structure. The MFGM PLs and proteins have a wide range of nutritional and health benefits. Animal and human studies have shown that consumption of MFGM preparations has beneficial effects on brain development and function and physical performance, particularly in the elderly. MFGM also improves intestinal health and skin condition, combats hypercholesterolaemia, obesity and inflammation. The present article updates the information about MFGM; particularly its nutritional and health benefits.

show abstract

“…Lactoferrin also has been reported to promote the growth of various lactobacilli [ 53 ]. Previously, milk-fat globule membrane glycoproteins have been shown to have anti-adhesive properties against a range of pathogens, including rotavirus and various enteric bacteria [ 54 , 55 ]. Notably, sialic acid on some milk glycoproteins have been implicated in binding E. coli and Salmonella enteritidis [ 43 , 56 ], increasing growth of the bifidobacterial spp.…”

Section: Discussionmentioning

confidence: 99%

A Whey Fraction Rich in Immunoglobulin G Combined with Bifidobacterium longum subsp. infantis ATCC 15697 Exhibits Synergistic Effects against Campylobacter jejuni

Quinn

Kilcoyne

Walsh

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Evidence that whey proteins and peptides have health benefits beyond basic infant nutrition has increased dramatically in recent years. Previously, we demonstrated that a whey-derived immunoglobulin G-enriched powder (IGEP) enhanced adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 (B. infantis) to HT-29 cells. In this study, we investigated the synergistic effect of IGEP-treated B. infantis on preventing the attachment of highly invasive Campylobacter jejuni 81–176 (C. jejuni) to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 48% compared to the control (non-IGEP-treated B. infantis). We also confirmed that treatment of IGEP with sodium metaperiodate, which disables the biological recognition of the conjugated oligosaccharides, reduced adhesion of B. infantis to the intestinal cells. Thus, glycosylation of the IGEP components may be important in enhancing B. infantis adhesion. Interestingly, an increased adhesion phenotype was not observed when B. infantis was treated with bovine serum-derived IgG, suggesting that bioactivity was unique to milk-derived immunoglobulin-rich powders. Notably, IGEP did not induce growth of B. infantis within a 24 hours incubation period, as demonstrated by growth curves and metabolite analysis. The current study provides insight into the functionality of bovine whey components and highlights their potential in positively impacting the development of a healthy microbiota.

show abstract

Defatted bovine milk fat globule membrane inhibits association of enterohaemorrhagic Escherichia coli O157:H7 with human HT-29 cells

Cited by 24 publications

References 59 publications

Milk Fat Globules Hamper Adhesion of Enterohemorrhagic Escherichia coli to Enterocytes: In Vitro and in Vivo Evidence

Milk Fat Globules Hamper Adhesion of Enterohemorrhagic Escherichia coli to Enterocytes: In Vitro and in Vivo Evidence

Milk fat globule membrane: An overview with particular emphasis on its nutritional and health benefits

A Whey Fraction Rich in Immunoglobulin G Combined with Bifidobacterium longum subsp. infantis ATCC 15697 Exhibits Synergistic Effects against Campylobacter jejuni

Contact Info

Product

Resources

About