Deeper Mitochondrial Sequencing Reveals Cryptic Diversity and Structure in Brazilian Green Turtle Rookeries

Shamblin, Brian M.; Dutton, Peter H.; Bjorndal, Karen A.; Bolten, Alan B.; Naro‐Maciel, Eugênia; Santos, Armando José Barsante; Bellini, Cláudio; Baptistotte, Cecília; Marcovaldi, Maria Ângela; Nairn, Campbell J.

doi:10.2744/ccb-1152.1

Cited by 28 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All but one had been previously described in populations in the Caribbean Sea, South America, South Atlantic (Ascension Island; Formia, Godley, Dontaine, & Bruford, ) and Africa (Shamblin, Bagley et al, ; Shamblin, Dutton et al, ). The haplotypes found in our samples belonged to different lineages as defined in the literature (Naro‐Maciel et al, ): most haplotypes belonged to lineage A (84%), which is typically found in the Caribbean, while the rest belonged to lineage B (Figure ) typically found in South America, South Atlantic and Africa (Patrício et al, ; Shamblin, Bagley et al, ; Shamblin, Dutton et al, ). CM‐A5.1 is the only haplotype of lineage B found shared by both the captive and the wild populations; however, this haplotype is not exclusive of the South Atlantic region and can be found in other wild populations of the Caribbean region (Naro‐Maciel et al, ).…”

Section: Resultsmentioning

confidence: 96%

“…South Caribbean populations are AVE = Aves Island (Venezuela) and SUR = Matapica (Suriname) (Bolker, Okuyama, Bjorndal, & Bolten, ). South Atlantic populations are ASC = Ascension Island, BIO = Bioko Island (Equatorial Guinea), SAO = Sao Tome (Formia et al, ), TI = Trinidad Island (Trinidad y Tobago), RA = Rocas Atoll and FN = Fernando de Noronha (Brazil), and POI = Poilao (Guinea Bissau) (Patrício et al, ; Shamblin, Dutton et al, )…”

Section: Resultsmentioning

confidence: 99%

“…Both D‐loop and STR sequences were also used to confirm maternal assignments resulting from microsatellite analysis. Using D‐loop sequences we performed analysis of wild and captive populations compared to other wild populations of the Caribbean (Shamblin, Bagley et al, ), African (Patrício et al, ; Shamblin, Dutton et al, ) and Mediterranean (Bradshaw et al, ) regions. Incorporating D‐loop sequences from these populations, we tested for genetic structuring ( F ST ) and genetic diversity.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

How many came home? Evaluating ex situ conservation of green turtles in the Cayman Islands

et al. 2019

View full text Add to dashboard Cite

Ex situ management is an important conservation tool that allows the preservation of biological diversity outside natural habitats while supporting survival in the wild. Captive breeding followed by re‐introduction is a possible approach for endangered species conservation and preservation of genetic variability. The Cayman Turtle Centre Ltd was established in 1968 to market green turtle (Chelonia mydas) meat and other products and replenish wild populations, thought to be locally extirpated, through captive breeding. We evaluated the effects of this re‐introduction programmme using molecular markers (13 microsatellites, 800‐bp D‐loop and simple tandem repeat mitochondrial DNA sequences) from captive breeders (N = 257) and wild nesting females (N = 57) (sampling period: 2013–2015). We divided the captive breeders into three groups: founders (from the original stock), and then two subdivisions of F1 individuals corresponding to two different management strategies, cohort 1995 (“C1995”) and multicohort F1 (“MCF1”). Loss of genetic variability and increased relatedness was observed in the captive stock over time. We found no significant differences in diversity among captive and wild groups, and similar or higher levels of haplotype variability when compared to other natural populations. Using parentage and sibship assignment, we determined that 90% of the wild individuals were related to the captive stock. Our results suggest a strong impact of the re‐introduction programmme on the present recovery of the wild green turtle population nesting in the Cayman Islands. Moreover, genetic relatedness analyses of captive populations are necessary to improve future management actions to maintain genetic diversity in the long term and avoid inbreeding depression.

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

How many came home? Evaluating ex situ conservation of green turtles in the Cayman Islands

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The green turtle colonization of the Mediterranean probably occurred within the last 10 000 years (Bowen et al, ), and the region was likely to have first been colonized by the more temperate loggerhead turtle ( C. caretta ) (Clusa et al, ). Thus, it was suggested that mtDNA markers were potentially less accurate for revealing differentiation at finer geographical scales or among recently diverged populations (Formia, Godley, Dontaine, & Bruford, ); however, the emergence of more polymorphic mtDNA markers (Tikochinski et al, ) opened up the possibility of using this maternally inherited molecule at finer temporal or geographical scales (Shamblin et al, ). Combining the mitochondrial STR haplotyping with the traditional control region haplotyping is helpful only if the latter is diverse enough (Shamblin et al, ).…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA short tandem repeats unveil hidden population structuring and migration routes of an endangered marine turtle

Tikochinski

Bradshaw

Mastrogiacomo³

et al. 2018

Aquatic Conservation

View full text Add to dashboard Cite

The assessment of the composition and dynamics of endangered populations is crucial for management and conservation, and appropriate genetic markers are critical. The genetic structuring of the Mediterranean green turtle (Chelonia mydas) populations and the origin of the stranded animals found along the Israeli coast was investigated using new highly polymorphic short tandem repeat (STR) markers. The structuring of nesting populations was studied using pairwise genetic distances and a principal coordinates analysis (PCoA). The contribution of the different nesting populations to the stranded sample was assessed by using a mixed‐stock analysis. A clear population genetic structure, not detected before, has been revealed. The four nesting populations are genetically well differentiated, and thus should be considered as different management units. The populations from Turkey and Israel showed higher resemblance, despite residing at opposite ends of the Mediterranean distribution. The Turkish nesting population is the main source of the stranded turtles sampled along the Israeli shore, confirming that individuals from this population migrate from north to south along the eastern shore of the Mediterranean, as previously shown by telemetry studies. The use of a highly polymorphic haplotyping method enabled the detection of a clear genetic structuring of the green turtle populations in the eastern Mediterranean Sea that was not revealed in previous studies, demonstrating the importance of marker selection in population genetics. The analysis of the genetic composition of the stranded turtles allowed us to investigate the migration patterns from nesting to foraging areas, supporting previous satellite‐tracking and stable‐isotope results. These results will help to delineate conservation management units for the species in the Mediterranean, and reveal connectivity among beaches and mixed aggregations.

show abstract

“…Examining differences in the frequencies of these mtSTR variants from the Israeli rookery compared to stranding's along the Israeli coast suggested that population structure is present among Mediterranean green turtle rookeries. In another example, Shamblin et al (2015c) combined mtSTR markers with sequencing of the extended control region from two northern Brazil island rookeries that were previously undifferentiated (Fernando de Noronha and Atol das Rocas; Bjorndal et al, 2006). This allowed them to discern cryptic diversity and clearly demonstrate that the two rookeries were demographically distinct populations, and also provide novel population markers for future MSA studies.…”

Section: Mitogenomic Sequencing and Mitochondrial Short Tandem Repeatsmentioning

confidence: 99%

Advances in the Application of Genetics in Marine Turtle Biology and Conservation

et al. 2017

Self Cite

View full text Add to dashboard Cite

Marine turtles migrate across long distances, exhibit complex life histories, and occupy habitats that are difficult to observe. These factors present substantial challenges to understanding fundamental aspects of their biology or assessing human impacts, many of which are important for the effective conservation of these threatened and endangered species. The early development and application of genetic tools made important contributions to understanding marine turtle population and evolutionary biology, such as providing evidence of regional natal homing by breeding adults, establishing connectivity between rookeries and foraging habitats, and determining phylogeography and broad scale stock structure for most marine turtle species. Recent innovations in molecular technologies, statistical methods, and creative application of genetic tools have significantly built upon this knowledge to address key questions in marine turtle biology and conservation management. Here, we evaluate the latest major advances and potential of marine turtle genetic applications, including improved resolution and large-scale syntheses of population structure, connectivity and phylogeography, estimation of key demographic rates such as age to maturity and operational or breeding sex ratios, insight into reproductive strategies and behavior, and assessment of differential human impacts among populations. We then discuss remaining challenges and emerging capabilities, such as rapid, multiplexed genotyping, and investigation of the genomic underpinnings of adaptive variation afforded by high-throughput sequencing technologies.

show abstract

Deeper Mitochondrial Sequencing Reveals Cryptic Diversity and Structure in Brazilian Green Turtle Rookeries

Cited by 28 publications

References 34 publications

How many came home? Evaluating ex situ conservation of green turtles in the Cayman Islands

How many came home? Evaluating ex situ conservation of green turtles in the Cayman Islands

Mitochondrial DNA short tandem repeats unveil hidden population structuring and migration routes of an endangered marine turtle

Advances in the Application of Genetics in Marine Turtle Biology and Conservation

Contact Info

Product

Resources

About