Deeper Insight into the Volatile Profile of Rosa willmottiae with Headspace Solid-Phase Microextraction and GC–MS Analysis

Abstract

As the distribution center of Rosa in the world, China has abundant wild germplasm resources, which can contribute to the breeding of modern roses. To explore the potential value of wild roses distributed in the Sichuan–Tibet region, solid phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) were used to determine the volatile organic compounds (VOCs) in Rosa willmottiae flowers at three flowering stages (bud stage, initial flowering stage, full flowering stage). Meanwhile, we compared the VOCs of R. willmottiae with different phenotypes (double flowers and single flowers). A total of 74 volatile compounds were identified. The results show that the essential substances belong to alcohols and terpenoids. The main volatile organic compounds are 2-phenyl ethanol (20.49%), benzyl alcohol (10.69%), β-maaliene (8.66%), geranyl acetate (8.47%), and (+)-α-long pinene (6.127%). Different flowering stages had great influence on the volatile profile, from the bud stage to full flowering stage; the content of terpenoids released decreased by 6.17%, whereas alcohols and esters increased by 8.58% and 11.56%, respectively. The chemical diversity and the content of the main components with a different phenotype were not significantly different. Our result will provide a theoretical basis for the development and utilization of Rosa willmottiae in Sichuan and Tibet.

Investigation of Volatile Organic Compounds in Aromatic Phalaenopsis Cultivars Using Gas Chromatography–Mass Spectrometry

“…In the same genus, different aromatic species or cultivars possesses common or different VOCs. The common VOC categories of flowers (i.e., terpenes, alcohols, and esters) have been reported in Cymbidium faber (Omata et al 1990), Dendrobium (Yang et al 2022), and Rosa (Jiao et al 2022) and were also detected in the native species Phal. violacea (Chuang et al 2017), Phal.…”

Analysis of Spatial-Temporal Variation in Floral Volatiles Emitted from Lagerstroemia caudata by Headspace Solid-Phase Microextraction and GC–MS

“…Floral volatile profiles vary across different plants and across different growth stages [ 28 ]. Such variation depends not only on the physiological levels of plants, but also on their growth and reproduction strategies [ 29 ]. Our results indicated that the opening of L. caudata flowers was closely related to the emission of volatile compounds, resulting in a diurnal pattern in scent emission.…”

Latest Improvements and Expanding Applications of Solid-Phase Microextraction

“…A large number of plants own distinctive odors. Analyzing these odors is expected to reveal unique biomarkers for species identification and provide important data for economic valuation. − HS-SPME is an excellent candidate for odor enrichment, and the mixed fibers including DVB/CAR/PDMS, CAR/PDMS, and PDMS/DVB are preferred due to the synergetic effects of absorbents and adsorbents. − It is worth mentioning that the hybrid composites of silica aerogel, preyssler-type polyoxometalate, and MIL-101­(Cr) have been fabricated as a highly porous fiber coating, which has been successfully used for the headspace extraction and identification of Ferulago angulata volatile components. Exerting a magnetic field on the prepared SPME fiber using a permanent magnet substantially improves the extraction efficiency for the volatile components, to an average of 73% …”