Deep Venous Thrombosis: Leukocyte Rheology at Baseline and after in vitro Activation

LoPresti, Rosalia; Ferrara, Filippo; Canino, Baldassare; Montana, M.; Caimi, Gregorio

doi:10.1159/000054132

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…In previous papers regarding several venous diseases, such as deep venous thrombosis [9,19], post-thrombotic syndrome [7,8] and leg venous ulcers [20], we demonstrated, especially after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and with N-formyl-methionyl-leucyl-phenilalanine (fMLP), an evident alteration of the two parameters that reflect the rheology of the polymorphonuclear cells: the membrane fluidity, examined employing the fluorescent probe TMA-DPH, and the cytosolic calcium concentration, explored using the fluorescent probe Fura-2AM. In subjects with deep venous thrombosis [9] and leg venous ulcers [20] we also found an altered profile of the polymorphonuclear integrins (CD11a, CD11b, CD11c and CD18) at baseline and after activation with PMA and fMLP.…”

Section: Discussionmentioning

confidence: 99%

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Caimi¹,

Ferrara²,

Montana

et al. 2015

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Venous leg ulcers are common in subjects with chronic venous insufficiency. The increased intraluminal pressure causes alteration of the skin microcirculation, leukocyte activation and release of proteolytic enzymes leading to ulceration. An impaired expression and activity of matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs) might influence extracellular matrix degradation and deposition in chronic venous ulcers with the failure of the healing process. Our aim was to evaluate plasma concentration of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) in subjects with venous leg ulcers before and after the compression therapy. We enrolled 36 subjects (12 men and 24 women, mean age 67.38 ± 12.7 yrs) with non-infected venous leg ulcers (CEAP C6), which underwent a color Duplex scan examination of the veins and arteries of the inferior limbs and were treated with a multi-layer bandaging system. The ulcer healing was obtained in 23 subjects only (9 men and 14 women). We evaluated, on fasting venous blood, the plasma levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 using ELISA kit, before and after the treatment. We observed a significant increase in plasma concentration of gelatinases and their inhibitors and in MMP-2/TIMP-2 ratio in subjects with leg ulcers in comparison with normal controls. In subjects with healed ulcers we found a decrease in MMP-9 and TIMP-1 levels and in MMP-2/TIMP-2 ratio compared to the baseline values, although higher levels of all the examined parameters in comparison with normal controls. In conclusion, plasma MMPs profile is impaired in subjects with venous leg ulcers and it improves after the healing, persisting anyway altered in respect to healthy controls.

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Section: Discussionmentioning

confidence: 99%

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Caimi¹,

Ferrara²,

Montana

et al. 2015

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“…Chemokines can be classified into four classes; CXC, CC, C and CX3C according to their structure. Previous studies have shown that a polymorphonuclear (PMN) activation in thrombosis is present, and that PMN activation may be important in the etiologies of thrombosis (14,15). CXC chemokines possess a potent chemotactic activity on neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Gene expression levels of cytokines in peripheral blood mononuclear cells from patients with pulmonary embolism

Duan

Wang

et al. 2013

Molecular Medicine Reports

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The aim of this study was to investigate the differential gene expression of cytokines in peripheral blood mononuclear cells (PBMCs) from patients with pulmonary embolism (PE) and controls. Twenty patients with PE and twenty control patients matched for gender and age with the PE group were recruited into the study. Human cDNA microarray analysis was used to detect differences in the expression of cytokine-associated genes between the two groups. In PE patients, the expression levels of the genes encoding IFNα5, IFNα6, IFNα8, IFNα14, IFNκ, IFNω1, IFNε1 and IFNγ were significantly lower compared with controls (P<0.05). The expression levels of the genes encoding IL1α, IL2, IL3, IL9, IL13, IL17β, IL19, IL22, IL23α, IL24, IL25 and IL31 were significantly lower (P<0.05), while IL10 and IL28A mRNA expression levels were higher in PE patients compared with controls (P<0.05). In PE patients, Cxcl1, Cxcl2, Cxcl6, Cxcl13 and Cxcl14 mRNAs were significantly upregulated (P<0.05), however, Cxcl10 mRNA was significantly downregulated (P<0.01). In PE patients, the mRNA expression levels of TNF superfamily members 1, 9 and 13, and TNF receptor superfamily members 1A, 1B, 9, 10B, 10C, 10D and 19L, were significantly upregulated (P<0.05), whereas TNF receptor superfamily members 11B, 19 and 25 were significantly downregulated compared with controls (P<0.05). The mRNA expression levels of granulocyte-macrophage colony‑stimulating factor, granulocyte colony-stimulating factor, erythropoietin, thrombopoietin and mast cell growth factor were significantly lower in PE patients compared with controls (P<0.05). In PE patients, the mRNA expression levels of a variety of cytokines were imbalanced and cellular immune function was downregulated compared with controls. Thus, PE patients may be more susceptible to infections caused by viruses, intracellular bacteria and parasites.

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“…Recent studies have suggested that leukocytes and erythrocytes play a role in the process of coagulation [3][4][5][6][7][8][9][10] and their presence is clearly observed in the anatomy of a venous clot, which consists of a laminar structure of erythrocytes and fibrin, permeated by large numbers of leukocytes. 11 A recent study, using a mouse model of flow restriction-induced deep vein thrombosis without endothelium damage, showed that blood monocytes and neutrophils provide an initiating stimulus for development of deep vein thrombosis.…”

Section: Introductionmentioning

confidence: 99%

Hematologic variables and venous thrombosis: red cell distribution width and blood monocyte count are associated with an increased risk

Rezende¹,

Lijfering²,

Rosendaal³

et al. 2013

Haematologica

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Odds ratios and their 95% confidence intervals (95% CI) were cal- open-access paper. doi:10.3324/haematol.2013 Recent studies suggest that leukocytes and erythrocytes play a role in coagulation. However, whether leukocytes, erythrocytes and other hematologic variables are associated with risk of venous thrombosis is not well known. To study this, we used data from 2473 patients with venous thrombosis and 2935 controls. The variables assessed were: total leukocytes, granulocytes, lymphocytes, monocytes, hematocrit, hemoglobin, erythrocytes and red cell indices (mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and red cell distribution width). We found a strong dose-response relation for higher red cell distribution width and monocyte count with risk of venous thrombosis, with odds ratios of 3.1 (95% confidence interval, 2.0-4.8) and 2.8 (95% confidence interval, 1.3-5.8), respectively, after adjustment for age, sex, C-reactive protein level, malignancy and co-morbidities. Monocyte count and red cell distribution width were associated with venous thrombosis even within reference ranges. A low monocyte count (<0.12x10 9 /L) was associated with a lower risk of venous thrombosis after full adjustment (odds ratios 0.6; 95% confidence interval, 0.4-0.8). In summary, high red cell distribution width and blood monocyte count, two parameters that are inexpensive and easily obtainable, were clearly associated with an increased risk of venous thrombosis. Future studies should evaluate the underlying mechanism and the use of these variables in prediction models for first and recurrent thrombosis. ©2013 Ferrata Storti Foundation. This is an Hematologic variables and venous thrombosis: red cell distribution width and blood monocyte count are associated with an increased risk

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Deep Venous Thrombosis: Leukocyte Rheology at Baseline and after in vitro Activation

Cited by 7 publications

References 18 publications

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Gene expression levels of cytokines in peripheral blood mononuclear cells from patients with pulmonary embolism

Hematologic variables and venous thrombosis: red cell distribution width and blood monocyte count are associated with an increased risk

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