Deep supercooling xylem parenchyma cells of katsura tree (<i>Cercidiphyllum japonicum</i>) contain flavonol glycosides exhibiting high anti‐ice nucleation activity

Kasuga, Jun; Hashidoko, Yasuyuki; Nishioka, Atsushi; Yoshiba, Megumi; Arakawa, Keita; Fujikawa, Seizo

doi:10.1111/j.1365-3040.2008.01835.x

Cited by 64 publications

(64 citation statements)

References 78 publications

(125 reference statements)

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“…Based our results, Kasuga et al 2008 found that some flavonol glycosides isolated from the xylem parenchyma cells had a high level of SFC activity. Furthermore, the hydrolyzable tannins in deep supercooling xylem cells in Cercidiphyllum japonicum exhibited the SFC activity like flavonol glycosides Wang et al, 2012 .…”

Section: Discussionmentioning

confidence: 91%

“…Even if the concentration of all the active compounds having the SCF activity is increased, their activities become constant at a certain concentration FIG.2, Kawahara et al, 1996a;Kawahara et al, 2000;Kasuga et al, 2008 . It was thought that this was due to the fact that the surface area of the ice nucleus was fixed.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the active compounds having SCF activity were polyphenols derived from plants or polyphenol glycosides in coniferous trees Kawahara et al, 1996a;Kasuga et al, 2008 . Polyphenol is a generic term for compounds having two or more hydroxyl groups -OH on a benzene ring.…”

Section: Screening Of Scf Activity From Hot Water Extracts Of Variousmentioning

confidence: 99%

“…Moreover, four kinds of flavonol glycosides with anti-ice nucleation activity have an important role in supercooling in the xylem parenchyma cells of katsura trees Cercidiphyllum japonicum Kasuga et al, 2008 . Furthermore, four kinds of hydrolysable gallotannins in the xylem parenchyma cells of katsura trees exhibited supercooling capabilities in the range of 1.5-4.5 , at a concentration of 1 mg/mL Wang D. et al, 2012 .…”

Section: The Preparation Of the Extract From Coffee Refusementioning

confidence: 99%

“…The temperature at which 50% of the drops froze was recorded T 50 . The silver iodide wThe hich has been used in the study of SCF materials Kawahara et al, 1996a;Kawahara et al, 2000;Kasuga et al, 2008 so far was used as the foreign matter of this ice nucleus. The silver iodide suspension 1 mg/ ml of 90 µl and the sample or distilled water of 10 µl were mixed, and kept at room temperature for 30 min.…”

Section: Measurement Of Scf Activity Anti-ice Nucleation Activitymentioning

confidence: 99%

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Characterization of Anti-Ice Nucleation Activity of the Extract from Coffee Refuse

et al. 2017

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The supercooling-facilitating SCF activities, that is, the anti-ice nucleation activity of the hot water extracts from five types of processed food refuse was examined. The extract with the highest activity among five hot water extracts was coffee refuse, showing 1.50 of SCF activity at a final concentration of 0.1 mg/ml. From the hot water extract of coffee refuse, the coffee refuse extract containing various polyphenols was prepared by the ultrafiltration less than MWCO 10,000 , a solvent fractionation of ethyl acetate. The yield of coffee refuse extract was 0.9% w/w from dried coffee refuse. The SCF activity of the coffee refuse extract at a final concentration of 1.0 mg/ml was 4.2 . HPLC analysis of the coffee refuse extract showed that caffeine and chlorogenic acid, which are major components of coffee, could be found at 173 and 62.3 µg/ml, respectively. However, the SCF activities of both compounds 0.70 and 1.06 at a final concentration of 0.1 mg/ml were lower than those of ferulic acid and coumaric acid, respectively at 3.40 and 2.35 . This is the first report to our knowledge on the SCF activity of caffeine. The SCF activity of caffeine at a final concentration of 1.0 mg/ml was 2.3 . The specificity of caffeine against various ice nuclei containing calcium oxalate, 9-fluorenon, and ice nucleating bacteria was examined. Caffeine at a final concentration of 1.0 mg/ml could inhibit the ice nucleation activity of calcium oxalate, and Pseudomonas fluorescens KUIN-1 at the same level that of as silver iodide. From these results, it was suggested that the extract could be able to be applied to the field to control the frost damage of the vegetables and that the harvested vegetables might be stored unfrozen even at 0 or less.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Screening Of Scf Activity From Hot Water Extracts Of Variousmentioning

confidence: 99%

Section: The Preparation Of the Extract From Coffee Refusementioning

confidence: 99%

Section: Measurement Of Scf Activity Anti-ice Nucleation Activitymentioning

confidence: 99%

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Characterization of Anti-Ice Nucleation Activity of the Extract from Coffee Refuse

et al. 2017

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Plant Responses to Freezing

Fujikawa

2016

Encyclopedia of Life Sciences

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During the course of evolution, plants have developed complex mechanisms to survive under a freezing condition. The ability of temperate plants to endure freezing differs depending on the season and depending on the region they inhabit in relation to the environmental temperatures. The prerequisite for survival of plants under a freezing condition is avoidance of lethal intracellular freezing in living cells. Depending on the function in living cells, such avoidance mechanisms change due to the difference in responses of cell walls as well as the plasma membrane to extracellular ice. Owing to these differences, living plant cells adapt to freezing by extracellular freezing and deep supercooling and also by extraorgan freezing, in which cells adapt by an intermediate form between extracellular freezing and deep supercooling. Key Concepts The first step of freezing resistance is the blocking of cell walls and plasma membranes against inoculation of extracellular ice. Living cells in most temperate plant tissues respond to freezing by extracellular freezing, in which cell walls inhibit ice penetration but allow dehydration. Interbilayer events, which are caused by the close approach of membranes by mechanical stress of freezing, are the main cause of injury by extracellular freezing. Many cold acclimation‐induced changes are related to inhibition of or reduction in the incidence of interbilayer events. Adaptation by deep supercooling occurs in cells with rigid and thick walls, which do not allow penetration of ice or dehydration. Supercooling capacity is changed by intracellular contents, especially by the interaction of antiice nucleation polyphenols with ice nucleators. Dormant buds of many woody plants respond to freezing by extraorgan freezing, in which formation of extracellular ice is excluded in tissues with freezing‐susceptible cells.

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Environmental Challenges for Trees

2017

Applied Tree Biology

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Deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides exhibiting high anti‐ice nucleation activity

Cited by 64 publications

References 78 publications

Characterization of Anti-Ice Nucleation Activity of the Extract from Coffee Refuse

Characterization of Anti-Ice Nucleation Activity of the Extract from Coffee Refuse

Plant Responses to Freezing

Environmental Challenges for Trees

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