Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol

Wang, Houmiao; Lei, Yong; Yan, Liying; Cheng, Ke; Dai, Xiaofeng; Wan, Ling‐Shu; Guo, Wanshou; Ling, Cheng; Liao, Boshou

doi:10.1186/s12866-015-0513-6

Cited by 45 publications

(53 citation statements)

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“…14 The soil fungi used in this research included five strains of Aspergillus that differed from each other by their metabolite profiles and toxigenic potential: A. flavus NRRL 3357, a moderate producer of aflatoxins B 1 (7) and B 2 (8); A. flavus NRRL 29487, a high producer of aflatoxins B 1 and B 2 ; A. nomius NRRL 13137, a high producer of aflatoxins B 1 , B 2 , G 1 (9), and G 2 (10); A. parasiticus NRRL 29580, a very high producer of aflatoxins B 1 , B 2 , G 1 , and G 2 ; and A. parasiticus NRRL 29602, a producer of O-methyl sterigmatocystin (11). The data on the fungal toxigenic potentials (not listed here) were provided by Dr. B. Horn of the National Peanut Research Laboratory, ARS, USDA (Dawson, GA).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In addition, this stilbenoid caused abnormal mycelial development and directly inhibited the expression of aflatoxin-biosyntheticpathway cluster genes. 9 Resveratrol content in resistant peanut lines was significantly higher than that in susceptible lines. At the same time, aflatoxin content was lower in the resistant lines compared with that in the susceptible lines.…”

Section: ■ Introductionmentioning

confidence: 99%

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Suppression of Aflatoxin Production in Aspergillus Species by Selected Peanut (Arachis hypogaea) Stilbenoids

Соболев

Arias

Goodman

et al. 2017

J. Agric. Food Chem.

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Aspergillus flavus is a soil fungus that commonly invades peanut seeds and often produces carcinogenic aflatoxins. Under favorable conditions, the fungus-challenged peanut plant produces and accumulates resveratrol and its prenylated derivatives in response to such an invasion. These prenylated stilbenoids are considered peanut antifungal phytoalexins. However, the mechanism of peanut−fungus interaction has not been sufficiently studied. We used pure peanut stilbenoids arachidin-1, arachidin-3, and chiricanine A to study their effects on the viability of and metabolite production by several important toxigenic Aspergillus species. Significant reduction or virtually complete suppression of aflatoxin production was revealed in feeding experiments in A. flavus, Aspergillus parasiticus, and Aspergillus nomius. Changes in morphology, spore germination, and growth rate were observed in A. flavus exposed to the selected peanut stilbenoids. Elucidation of the mechanism of aflatoxin suppression by peanut stilbenoids could provide strategies for preventing plant invasion by the fungi that produce aflatoxins.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Suppression of Aflatoxin Production in Aspergillus Species by Selected Peanut (Arachis hypogaea) Stilbenoids

Соболев

Arias

Goodman

et al. 2017

J. Agric. Food Chem.

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“…Complementary DNA (cDNA) libraries were built as described previously [23]. Briefly, the mRNA in each sample was fragmented into short sequences with divalent cations by heating in a proprietary buffer.…”

Section: Cdna Library Construction and Sequencing Using The Illumina mentioning

confidence: 99%

“…We found that Eha is required for the bacteria to resist oxidative stress and survive in macrophages [16]. As the type III secretion system in E. tarda disturbs the formation of the acidic environment in phagosomes [9], we hypothesized that Eha may be required for the bacteria to resist the acid stress in macrophages.Next generation sequencing technology, RNA-Sequencing (RNAseq), has been used in bacterial transcriptome analyses [17]. We performed a high-throughput RNA-seq study, which has firstly been applied in the transcriptome analyses of E. tarda, to detect and compare the differentially-expressed genes (DEGs) between the ET13 wild type bacterium and its eha mutant following exposure to acid stress.…”

mentioning

confidence: 99%

“…Next generation sequencing technology, RNA-Sequencing (RNAseq), has been used in bacterial transcriptome analyses [17]. We performed a high-throughput RNA-seq study, which has firstly been applied in the transcriptome analyses of E. tarda, to detect and compare the differentially-expressed genes (DEGs) between the ET13 wild type bacterium and its eha mutant following exposure to acid stress.…”

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confidence: 99%

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Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Gao¹,

Liu²,

Y³

et al. 2017

Metabolomics

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In response to stresses in host cells, several transcriptional regulators described in E. tarda can activate a series of interacting signaling networks [13]. Our previous study found that Eha is a transcriptional regulator of the MarR family [14]. Eha could also regulate the mRNA levels of some surface structures like the type III secretion system to affect the intracellular survival and virulence of E. tarda [15]. We found that Eha is required for the bacteria to resist oxidative stress and survive in macrophages [16]. As the type III secretion system in E. tarda disturbs the formation of the acidic environment in phagosomes [9], we hypothesized that Eha may be required for the bacteria to resist the acid stress in macrophages.Next generation sequencing technology, RNA-Sequencing (RNAseq), has been used in bacterial transcriptome analyses [17]. We performed a high-throughput RNA-seq study, which has firstly been applied in the transcriptome analyses of E. tarda, to detect and compare the differentially-expressed genes (DEGs) between the ET13 wild type bacterium and its eha mutant following exposure to acid stress. Our present study provides the gene expression profiles and sheds light on the molecular mechanism by which E. tarda survival is regulated by Eha under acid stress conditions. Key words: Eha gene; E. tarda; RNA-seq; Macrophage; Acidification IntroductionEdwardsiella tarda (E. tarda) is a facultative intracellular pathogen that causes Edwardsiellosis in freshwater and marine fish worldwide and a Salmonella-like gastroenteritis in humans [1,2]. Macrophages play critical roles in the defense against invading bacteria. Zhang et al. showed that E. tarda can utilize macrophages as a niche to initiate its virulence and spread systemic infections [3]. However, there are various stressful conditions such as nutrition deprivation, low pH and high reactive oxygen species (ROS) present in the intracellular niche of the phagocytes [4]. There are the strategies that virulent bacterial strains use to evade phagosomes and escape into the cytosol to enable their survival in the cells [5]. E. tarda could live and multiply in macrophages, and escape from the cells. It is important for the bacterium to lead to extra intestinal diseases and systemic infections [6]. E. tarda is capable to detoxify ROS by generating catalase (Kat) and superoxide dismutase (Sod) to survive within macrophages [7,8]. Okuda et al. suggested that the bacterial type III secretion system is able to interfere with the formation of acid stress in phagosomes to facilitate its replication in macrophages [9]. However, little other information is available about how E. tarda is affected by an acidic environment.Some findings have been reported about the changes that occur in other intracellular pathogens in response to acid stress in macrophages. For example, Rathman et al. suggested that an acidic environment in Salmonella-containing phagosomes is necessary for the bacterial survival and replication within the macrophage [10]. Supporting this finding, ...

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Manuscript title: antifungal proteins from moulds: analytical tools and potential application to dry-ripened foods

Delgado

Owens

Doyle

et al. 2016

Appl Microbiol Biotechnol

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Moulds growing on the surface of dry-ripened foods contribute to their sensory qualities, but some of them are able to produce mycotoxins that pose a hazard to consumers. Small cysteine-rich antifungal proteins (AFPs) from moulds are highly stable to pH and proteolysis and exhibit a broad inhibition spectrum against filamentous fungi, providing new chances to control hazardous moulds in fermented foods. The analytical tools for characterizing the cellular targets and affected pathways are reviewed. Strategies currently employed to study these mechanisms of action include 'omics' approaches that have come to the forefront in recent years, developing in tandem with genome sequencing of relevant organisms. These techniques contribute to a better understanding of the response of moulds against AFPs, allowing the design of complementary strategies to maximize or overcome the limitations of using AFPs on foods. AFPs alter chitin biosynthesis, and some fungi react inducing cell wall integrity (CWI) pathway. However, moulds able to increase chitin content at the cell wall by increasing proteins in either CWI or calmodulin-calcineurin signalling pathways will resist AFPs. Similarly, AFPs increase the intracellular levels of reactive oxygen species (ROS), and moulds increasing G-protein complex β subunit CpcB and/or enzymes to efficiently produce glutathione may evade apoptosis. Unknown aspects that need to be addressed include the interaction with mycotoxin production by less sensitive toxigenic moulds. However, significant steps have been taken to encourage the use of AFPs in intermediate-moisture foods, particularly for mould-ripened cheese and meat products.

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Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol

Cited by 45 publications

References 100 publications

Suppression of Aflatoxin Production in Aspergillus Species by Selected Peanut (Arachis hypogaea) Stilbenoids

Suppression of Aflatoxin Production in Aspergillus Species by Selected Peanut (Arachis hypogaea) Stilbenoids

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Manuscript title: antifungal proteins from moulds: analytical tools and potential application to dry-ripened foods

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