Deep-Sea Anemones Are Prospective Source of New Antimicrobial and Cytotoxic Compounds

Kvetkina, Aleksandra; Костина, Е. Э.; Chausova, Victoria; Yurchenko, Ekaterina A.; Bakunina, I. Yu.; Пивкин, М. В.; Anastyuk, Stanislav D.; Popov, Roman S.; Monastyrnaya, Margarita; Kozlovskaya, É. P.; Isaeva, Marina; Dmitrenok, Pavel S.; Leychenko, Elena

doi:10.3390/md19120654

Cited by 9 publications

(10 citation statements)

References 82 publications

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“…Sea anemone venoms are known as rich sources of bioactive components, encompassing different types of proteins, peptides, and nonproteinaceous compounds. Although a great diversity is found, most of their components remain poorly explored [4,19]. Among the proteins and peptides, neurotoxins represent a major class of active molecules, acting on different structures, such as potassium, sodium, acid-sensing ion channels (ASIC), and other targets [3].…”

Section: Discussionmentioning

confidence: 99%

A Tale of Toxin Promiscuity: The Versatile Pharmacological Effects of Hcr 1b-2 Sea Anemone Peptide on Voltage-Gated Ion Channels

et al. 2022

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Sea anemones are a rich source of biologically active compounds. Among approximately 1100 species described so far, Heteractis crispa species, also known as sebae anemone, is native to the Indo-Pacific area. As part of its venom components, the Hcr 1b-2 peptide was first described as an ASIC1a and ASIC3 inhibitor. Using Xenopus laevis oocytes and the two-electrode voltage-clamp technique, in the present work we describe the remarkable lack of selectivity of this toxin. Besides the acid-sensing ion channels previously described, we identified 26 new targets of this peptide, comprising 14 voltage-gated potassium channels, 9 voltage-gated sodium channels, and 3 voltage-gated calcium channels. Among them, Hcr 1b-2 is the first sea anemone peptide described to interact with isoforms from the Kv7 family and T-type Cav channels. Taken together, the diversity of Hcr 1b-2 targets turns this toxin into an interesting tool to study different types of ion channels, as well as a prototype to develop new and more specific ion channel ligands.

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Section: Discussionmentioning

confidence: 99%

A Tale of Toxin Promiscuity: The Versatile Pharmacological Effects of Hcr 1b-2 Sea Anemone Peptide on Voltage-Gated Ion Channels

et al. 2022

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“…The 18S rRNA and COI genes, and ITS gene region were amplified and sequenced as described in [ 67 ]. Gene sequences were deposited in GenBank under accession numbers ON797294 (for H. magnifica 116) and ON926908 (for H. magnifica 91) for the 18S rRNA genes; ON797309 (for H. magnifica 116) and OP107886 (for H. magnifica 91) for the COI gene; ON831386-ON831387 (for H. magnifica 116) and ON936908-ON936909 (for H. magnifica 91) for the ITS genes region.…”

Section: Methodsmentioning

confidence: 99%

Nicotinic Acetylcholine Receptors Are Novel Targets of APETx-like Toxins from the Sea Anemone Heteractis magnifica

Kalina

Kasheverov

Koshelev

et al. 2022

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The nicotinic acetylcholine receptors (nAChRs) are prototypical ligand-gated ion channels, provide cholinergic signaling, and are modulated by various venom toxins and drugs in addition to neurotransmitters. Here, four APETx-like toxins, including two new toxins, named Hmg 1b-2 Metox and Hmg 1b-5, were isolated from the sea anemone Heteractis magnifica and characterized as novel nAChR ligands and acid-sensing ion channel (ASIC) modulators. All peptides competed with radiolabeled α-bungarotoxin for binding to Torpedo californica muscle-type and human α7 nAChRs. Hmg 1b-2 potentiated acetylcholine-elicited current in human α7 receptors expressed in Xenopus laevis oocytes. Moreover, the multigene family coding APETx-like peptides library from H. magnifica was described and in silico surface electrostatic potentials of novel peptides were analyzed. To explain the 100% identity of some peptide isoforms between H. magnifica and H. crispa, 18S rRNA, COI, and ITS analysis were performed. It has been shown that the sea anemones previously identified by morphology as H. crispa belong to the species H. magnifica.

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“…The species of sea anemone was identified morphologically by Dr. E. Kostina (A.V. Zhirmunsky National Scientific Center of Marine Biology FEB RAS) as well as phylogenetically on the basis of nuclear and mitochondrial markers [ 24 ]. Sea anemones were frozen and kept at −20 °C.…”

Section: Methodsmentioning

confidence: 99%

First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone Stomphia coccinea

et al. 2022

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A novel peptide AnmTX Sco 9a-1 with the β-hairpin fold was isolated from the swimming sea anemone Stomphia coccinea (Actinostolidae family). The peptide consists of 28 amino acid residues, including modified hydroxyproline residue, and its measured molecular mass is 2960 Da. The peptide was not toxic on mice; however, it stimulated their exploratory motivation and active search behavior, and demonstrated an anti-anxiety effect. AnmTX Sco 9a-1 at doses of 0.1 and 1 mg/kg reduced the volume of edema during 24 h better than the nonsteroidal anti-inflammatory drug, Diclofenac, at dose of 1 mg/kg in a model of acute local λ-carrageenan-induced inflammation. ELISA analysis of the animal’s blood showed that peptide at a dose of 1 mg/kg reduced the content of tumor necrosis factor-α (TNF-α), a pro-inflammatory mediator responsible in the edema development, up to the level of TNF-α in the intact group. Besides, AnmTX Sco 9a-1 demonstrated a significant analgesic effect on acute pain sensitivity in the carrageenan-induced thermal hyperalgesia model at doses of 0.1 and 1 mg/kg. Activity of AnmTX Sco 9a-1 was shown not to be associated with modulation of nociceptive ASIC channels.

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Deep-Sea Anemones Are Prospective Source of New Antimicrobial and Cytotoxic Compounds

Cited by 9 publications

References 82 publications

A Tale of Toxin Promiscuity: The Versatile Pharmacological Effects of Hcr 1b-2 Sea Anemone Peptide on Voltage-Gated Ion Channels

A Tale of Toxin Promiscuity: The Versatile Pharmacological Effects of Hcr 1b-2 Sea Anemone Peptide on Voltage-Gated Ion Channels

Nicotinic Acetylcholine Receptors Are Novel Targets of APETx-like Toxins from the Sea Anemone Heteractis magnifica

First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone Stomphia coccinea

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