Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging

Goltsev, Yury; Samusik, Nikolay; Kennedy‐Darling, Julia; Bhate, Salil; Hale, Matthew B.; Vásquez, Gustavo; Nolan, Garry P.

doi:10.2139/ssrn.3155542

Cited by 212 publications

(350 citation statements)

References 23 publications

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“…Cycles of labelling, imaging and removing reporters are performed by a fully automated fluidics system and images are collected and compiled across all the cycles to achieve single‐cell resolution [83]. The platform is compatible with the existing three‐colored fluorescence microscope, enabling the conversion of a simple fluorescence microscope into a tool for multiparametric imaging and cytometry [84].…”

Section: Brightfield‐based Mihc/ifmentioning

confidence: 99%

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Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

Tan

Nerurkar

Cai

et al. 2020

Cancer Communications

357

268

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Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter-observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell-cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.

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Section: Brightfield‐based Mihc/ifmentioning

confidence: 99%

“…CODEX antibodies have demonstrated comparable staining patterns when compared to other IF and IHC grade dye‐conjugated antibodies [84, 85]. The compatibility of the system with various tissue types including spleen, tonsil, lymph node and FFPE melanoma has been demonstrated [85].…”

Section: Brightfield‐based Mihc/ifmentioning

confidence: 99%

Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

Tan

Nerurkar

Cai

et al. 2020

Cancer Communications

357

268

View full text Add to dashboard Cite

show abstract

“…New methods try to overcome this limitation by using multiplexed fluorescence cytometry or mass spectrometry. A technique for multiplexed imaging in fresh/frozen tissue sections, termed co‐detection by indexing (CODEX), was recently introduced that uses DNA barcodes, fluorescent dNTP analogs, and an in situ indexing procedure . Imaging techniques that rely on fluorescence‐tagged antibodies, like flow cytometry, are limited by spectral overlap.…”

Section: Imagingmentioning

confidence: 99%

Computational approaches for characterizing the tumor immune microenvironment

2019

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Summary Recent advances in high‐throughput molecular profiling technologies and multiplexed imaging platforms have revolutionized our ability to characterize the tumor immune microenvironment. As a result, studies of tumor‐associated immune cells increasingly involve complex data sets that require sophisticated methods of computational analysis. In this review, we present an overview of key assays and related bioinformatics tools for analyzing the tumor‐associated immune system in bulk tissues and at the single‐cell level. In parallel, we describe how data science strategies and novel technologies have advanced tumor immunology and opened the door for new opportunities to exploit host immunity to improve cancer clinical outcomes.

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“…Although we expect submissions using flow cytometry technology, we encourage the submission of manuscripts describing emerging and new technologies for single cell analysis. This includes all quantitative assays to analyze single cells including imaging and image cytometry , mass and imaging mass cytometry , single‐cell expressomics (RNA expression) , proteomics and genomics, and combinations of these technologies. The assays used include single‐cell immunophenotyping, intra‐ and extracellular markers (cytokines, proteins, and signal transduction pathways), optical and physiological properties, morphological features (size, shape, location in a tissue), RNA expression and sequence, and combinations of these parameters.…”

Section: Publication Policymentioning

confidence: 99%