Deep learning-assisted low-cost autofluorescence microscopy for rapid slide-free imaging with virtual histological staining
Ivy H. M. Wong,
Zhenghui Chen,
Lulin Shi
et al.
Abstract:Slide-free imaging techniques have shown great promise in improving the histological workflow. For example, computational high-throughput autofluorescence microscopy by pattern illumination (CHAMP) has achieved high resolution with a long depth of field, which, however, requires a costly ultraviolet laser. Here, simply using a low-cost light-emitting diode (LED), we propose a deep learning-assisted framework of enhanced widefield microscopy, termed EW-LED, to generate results similar to CHAMP (the learning tar… Show more
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