Deep eutectic solvents as novel extraction media for protein partitioning

Zeng, Qun; Wang, Yuzhi; Huang, Yanhua; Ding, Xueqin; Chen, Jing; Xu, Kaijia

doi:10.1039/c3an02235h

Cited by 207 publications

(123 citation statements)

References 26 publications

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“…Among these solvents, ionic liquids (ILs) have generated greater attention recently in the global scientific communities with the aim of developing ecofriendly solvents, because ILs possess attractive properties such as non-volatility (Grasvik et al 2014) and tailor-made reagents for selective extractions (Xia et al 2014;Zeng et al 2014) with lower melting points, in general, below 100°C which makes easy handling of IL solvents. However, synthesis of ILs is a cost-intensive process due to the high production costs of the individual IL components.…”

Section: Introductionmentioning

confidence: 99%

Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue

Kumar

Parikh

Mohanty

2015

Environ Sci Pollut Res

423

182

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The present investigation demonstrated pretreatment of lignocellulosic biomass rice straw using natural deep eutectic solvents (NADESs), and separation of high-quality lignin and holocellulose in a single step. Qualitative analysis of the NADES extract showed that the extracted lignin was of high purity (>90 %), and quantitative analysis showed that nearly 60 ± 5 % (w/w) of total lignin was separated from the lignocellulosic biomass. Addition of 5.0 % (v/v) water during pretreatment significantly enhanced the total lignin extraction, and nearly 22 ± 3 % more lignin was released from the residual biomass into the NADES extract. X-ray diffraction studies of the untreated and pretreated rice straw biomass showed that the crystallinity index ratio was marginally decreased from 46.4 to 44.3 %, indicating subtle structural alterations in the crystalline and amorphous regions of the cellulosic fractions. Thermogravimetric analysis of the pretreated biomass residue revealed a slightly higher T dcp (295 °C) compared to the T dcp (285 °C) of untreated biomass. Among the tested NADES reagents, lactic acid/choline chloride at molar ratio of 5:1 extracted maximum lignin of 68 ± 4 mg g(-1) from the rice straw biomass, and subsequent enzymatic hydrolysis of the residual holocellulose enriched biomass showed maximum reducing sugars of 333 ± 11 mg g(-1) with a saccharification efficiency of 36.0 ± 3.2 % in 24 h at 10 % solids loading.

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Section: Introductionmentioning

confidence: 99%

Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue

Kumar

Parikh

Mohanty

2015

Environ Sci Pollut Res

423

182

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“…16 Albeit scarcely investigated, the potential of DES as phase forming components of ABS was recently reported by Xu and co workers. 17,18 Yet, those pioneering phenomenological results did not comprise the mechanisms associated with the coexisting phases demixing and the stability of the HBD:HBA complex in aqueous media. Only "ternary" phase diagrams for each ABS were reported, and their applications to the extraction of proteins were presented.…”

Section: −10mentioning

confidence: 99%

“…Only "ternary" phase diagrams for each ABS were reported, and their applications to the extraction of proteins were presented. 17,18 Whether DES in the form of a HBD:HBA complex will form a ternary system, as in common ABS, or the alleged DES based ABS is a quaternary system that phase separates with a nonstoichiometric partition of the HBD and HBA species between the two phases remains an open question. In this context, the present work aims at shedding light on this subject.…”

Section: −10mentioning

confidence: 99%

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

Passos

Tavares

Ferreira

et al. 2016

ACS Sustainable Chem. Eng.

182

107

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Deep eutectic solvents (DES) have emerged in the past few years as a new class of solvents with promising applications in several fields. In the present work, the application of DES (formed by binary mixtures of cholinium chloride and carboxylic acids or urea) as phase forming components of aqueous biphasic systems (ABS) is inves tigated. The mechanisms associated with the phases demixing of ABS composed of DES, as well as the DES stability in aqueous solutions, are investigated to address the critical question whether DES based ABS are in fact ternary or quaternary mixtures. It is shown that the DES integrity is destroyed in ABS by the disruption of the hydrogen bonding interactions of the complex (a result of the isolated components preferential solvation by water), and as confirmed by a nonstoichiometric partition of the DES components between the coexisting phases. As a result, there are no "real" DES based ABS; instead, there is the formation of ABS composed of four components, where the carboxylic acid used as the hydrogen bond donor species seems to act as an additive. Finally, it is shown that these ABS have an outstanding potential to be used in extraction processes, as it is here demonstrated with the complete separation of two dyes. However, the volatile nature of short chain carboxylic acids and the nonstoichiometric partition of the DES components in ABS make the development of recovery and recycling steps more difficult to accomplish. The formation of DES results from the formation of strong hydrogen bonds between the two starting materials, namely, a hydrogen bond donor (HBD) and a hydrogen bond acceptor (HBA). The formation of ion−HBD complexes leads to a lower entropic difference of the phase transition and to the further depression of the freezing temperature.5−7 The first reported DES (composed of [N 111(2OH) ]Cl and urea) 1 remains the most widely investigated. However, DES can be formed by mixing other substituted tetralkylammonium or phosphonium salts with a HBD, such as amines, carboxylic acids, and carbohydrates, among others. 8−10Many DES share some of the unique characteristics of ionic liquids (ILs), namely, a low volatility, high conductivity, wide liquid temperature range, and high solvation ability for a large number of compounds.3,4 DES are also able to overcome some of the disadvantages related to ILs. They are easier to prepare, by a simple mixture of the starting materials at moderate temperatures, without requiring a reaction step. Furthermore, the addition of an agent able to disrupt hydrogen bonding interactions can lead to the recovery of at least one of the initial compounds. 3,4,11,12 The starting materials are usually cheaper, toxicologically well characterized, and often derived from renewable resources, which results in low cost and more environmentally friendly products. 3,4,11,12 As in ILs, innumer ous combinations of the starting materials can be attempted, and therefore, DES may also be classified as "designer solvents". Since DES have fewer restrictions ...

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“…DLLME-SFO is becoming a popular sample preparation technique, and the type of extraction solvents has been developed in recent years, e.g. [15] and has gradually been considered a new type of green ionic liquid [16][17][18][19]. DESs are composed of two or three components with a melting point lower than its individual components, consisting of hydrogen bond donor (HBD), and hydrogen bond acceptor (HBA) molecules in a certain molar ratio [20].…”

Section: Introductionmentioning

confidence: 99%

Dispersive liquid–liquid microextraction based on the solidification of deep eutectic solvent for the determination of benzoylureas in environmental water samples

Zeng

Qiao

et al. 2017

J of Separation Science

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We present a novel dispersive liquid-liquid microextraction method based on the solidification of deep eutectic solvent coupled with high-performance liquid chromatography with a variable-wavelength detection for the detection of five benzoylureas in real water samples. In this work, a green solvent consisting of 1-octyl-3-methylimidazolium chloride and 1-dodecanol was used as an extraction solvent, yielding the advantages of material stability, low density, and a suitable freezing point near room temperature. Parameters that significantly affect extraction efficiency were optimized by the one-factor-at-a-time approach. Under optimal conditions, the recoveries of five target compounds were obtained ranging from 87.39 to 98.05% with correlation coefficients ranging from 0.9994 to 0.9997 for pure water. The limits of detection were in the range of 0.09-0.16 μg/L. The enrichment factors were in the range of 171-188. Linearities were achieved in the range of 0.5-500 μg/L. The proposed method was successfully applied to determine benzoylureas in environmental water samples with a satisfactory recovery of approximately 81.38-97.67%.

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Deep eutectic solvents as novel extraction media for protein partitioning

Cited by 207 publications

References 26 publications

Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue

Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

Dispersive liquid–liquid microextraction based on the solidification of deep eutectic solvent for the determination of benzoylureas in environmental water samples

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