Deep eutectic solvents – a new additive in the encapsulation of lipase B from <i>Candida antarctica</i>: biocatalytic applications

Dudu, Adrian Ioan; Bencze, László Csaba; Paizs, Csaba; Toşa, Monica Ioana

doi:10.1039/d1re00469g

Cited by 2 publications

(1 citation statement)

References 35 publications

(33 reference statements)

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“…For this reason, herein, DESs were explored as alternative solvents for biocatalytic processes both in aqueous and non-aqueous reaction systems. As a biocatalyst, lipase from Candida antarctica B was used, one of the first enzymes exploited in DES-based processes and widely studied for its diverse biotechnological applications [26]. The incorporation of DESs into continuous biocatalytic processes in microreactor systems has been recently exerted by experts in the field of the application of DESs in biocatalysis [18,27].…”

Section: Introductionmentioning

confidence: 99%

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

et al. 2022

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In this study, 3D printing technology was exploited for the development of immobilized enzyme microreactors that could be used for biocatalytic processes in Deep Eutectic Solvent (DES)-based media. 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with polyethylenimine (PEI) and lipase from Candida antarctica (CALB) was covalently immobilized in the interior of each structure. DESs were found to have a negligible effect on the activity and stability of CALB, and the system proved highly stable and reusable in the presence of DESs for the hydrolysis of p-nitrophenyl butyrate (p-NPB). A kinetic study under flow conditions revealed an enhancement of substrate accessibility in the presence of Betaine: Glycerol (Bet:Gly) DES, while the system was not severely affected by diffusion limitations. Incubation of microreactors in 100% Bet:Gly preserved the enzyme activity by 53% for 30 days of storage at 60 °C, while the buffer-stored sample had already been deactivated. The microfluidic enzyme reactor was efficiently used for the trans-esterification of ethyl ferulate (EF) with glycerol towards the production of glyceryl ferulate (GF), known for its antioxidant potential. The biocatalytic process under continuous flow conditions exhibited 23 times higher productivity than the batch reaction system. This study featured an effective and robust biocatalytic system with immobilized lipase that can be used both in hydrolytic and synthetic applications, while further optimization is expected to upgrade the microreactor system performance.

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Section: Introductionmentioning

confidence: 99%

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

et al. 2022

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Predicting the formation of NADES using a transformer-based model

Ayres,

Gomez,

Silva

et al. 2024

Sci Rep

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The application of natural deep eutectic solvents (NADES) in the pharmaceutical, agricultural, and food industries represents one of the fastest growing fields of green chemistry, as these mixtures can potentially replace traditional organic solvents. These advances are, however, limited by the development of new NADES which is today, almost exclusively empirically driven and often derivative from known mixtures. To overcome this limitation, we propose the use of a transformer-based machine learning approach. Here, the transformer-based neural network model was first pre-trained to recognize chemical patterns from SMILES representations (unlabeled general chemical data) and then fine-tuned to recognize the patterns in strings that lead to the formation of either stable NADES or simple mixtures of compounds not leading to the formation of stable NADES (binary classification). Because this strategy was adapted from language learning, it allows the use of relatively small datasets and relatively low computational resources. The resulting algorithm is capable of predicting the formation of multiple new stable eutectic mixtures (n = 337) from a general database of natural compounds. More importantly, the system is also able to predict the components and molar ratios needed to render NADES with new molecules (not present in the training database), an aspect that was validated using previously reported NADES as well as by developing multiple novel solvents containing ibuprofen. We believe this strategy has the potential to transform the screening process for NADES as well as the pharmaceutical industry, streamlining the use of bioactive compounds as functional components of liquid formulations, rather than simple solutes.

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Deep eutectic solvents – a new additive in the encapsulation of lipase B from Candida antarctica: biocatalytic applications

Abstract: The enzymes encapsulation in sol-gel matrix can be improved using some additive acting on enzyme activity and/or stability. Ionic liquids, poly-hydroxy compounds, sugars, etc. have been previously reported as additives....

Cited by 2 publications

References 35 publications

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

Predicting the formation of NADES using a transformer-based model

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