Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation

Park, Kwan Seob; Shin, Jun Geun; Qureshi, Muhammad Mohsin; Chung, Euiheon; Eom, Tae Joong

doi:10.1038/s41598-018-29975-6

Cited by 32 publications

(32 citation statements)

References 21 publications

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“…To capture eTC-PCT images, a near-infrared laser beam was used which provides high penetration depth within biological tissue 19 . Higher penetration depth can be achieved using longer excitation wavelength 2,3 . In the case of tumor imaging, the high penetration of 808 nm enables photons to be transmitted into tissue and to be absorbed by the vascular network surrounding the tumor.…”

Section: Discussionmentioning

confidence: 99%

Non-invasive in-vivo 3-D imaging of small animals using spatially filtered enhanced truncated-correlation photothermal coherence tomography

Tavakolian

Roointan

Mandelis

2020

Sci Rep

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We present enhanced truncated-correlation phototothermal coherence tomography (eTC-PCT) for non-invasive three-dimensional imaging of small animals. Tumor detection is reported in a mouse thigh by injecting cancerous cells in the thigh followed by eTC-PCT imaging. Detection of the tumor 3 days after injection may lead to potential for using the eTC-PCT method for cancer treatment studies. eTC-PCT was also applied successfully to non-invasive in-vivo mouse brain structural imaging. A unique spatial-gradient-gate adaptive filter was introduced in a scanned mode along the (x,y) coordinates of camera images from different sub-cranial depths, revealing absorber true spatial extent from diffusive photothermal images and restoring pre-diffusion lateral image resolution beyond the Rayleigh criterion limit in diffusion-wave imaging science. The spatial resolution and contrast enhancement demonstrated in photothermal in-vivo and ex-vivo images of the mouse brain revealed not only vascular structures but also other brain structures, such as the brain hemispheres, cerebellum, and olfactory lobes. Optical imaging provides intrinsic advantages in biological tisssue characterization through the capacity for high image contrast and resolution. However, purely optical methods are hindered by their penetration depth being effectively limited to the optical diffusion length. Recently, two-photon microscopy 1 , three-photon microscopy 2 , and optical coherence tomography 3 were utilized for in-vivo brain imaging with the depth range of ~ 1.6 mm within the cortex layer and resolution of a few micrometers. This, however, was only possible in an invasive mode, after removing the skin and removing/thinning the skull. Although the image resolution of purely optical imaging methods is very high, the invasive nature of these methods limit their application for e.g. drug testing. Recently, photoacoustic tomography (PAT) has been explored for non-invasive cancer tumor imaging 4-7 and brain imaging applications as an alternative to purely optical imaging 8-13. Through exploiting the optical-to-ultrasonic energy conversion, photoacoustic methods combine high optical contrast with the superior penetration depth of ultrasonic waves. However, the reported setups require animal models to be surrounded by a coupling medium, often water, thus limiting the potential use of PAT. They also require the use of single transducer or array scanning which complicates the instrumentation and the image acquisition process. Light absorption and nonradiative energy conversion in the sample leads to the photothermal effect, which gives rise to thermophotonic imaging, an emerging photothermal diagnostic modality. It involves the detection of photothermal waves through emitted thermal infrared (IR) photons (Planck radiation) from tissues, captured by a mid-IR (MIR) camera. Enhanced Truncated-Correlation Photothermal Coherence Tomography (eTC-PCT) 14 is a novel imaging method based on the photothermal effect, which has achieved superior penetration

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Section: Discussionmentioning

confidence: 99%

Non-invasive in-vivo 3-D imaging of small animals using spatially filtered enhanced truncated-correlation photothermal coherence tomography

Tavakolian

Roointan

Mandelis

2020

Sci Rep

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“…Park et al [151] demonstrated the feasibility of non-invasive deep brain vascular imaging and reported the use of microvascular imaging in the hippocampal formation of mice using a 1.7 μm swept-source OCT system. The imaging results demonstrated that the proposed system can visualize blood flow at different locations of the hippocampus corresponding with deep brain areas.…”

Section: Oct Studies On the Brainmentioning

confidence: 99%

Optics Based Label-Free Techniques and Applications in Brain Monitoring

et al. 2020

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Functional near-infrared spectroscopy (fNIRS) has been utilized already around three decades for monitoring the brain, in particular, oxygenation changes in the cerebral cortex. In addition, other optical techniques are currently developed for in vivo imaging and in the near future can be potentially used more in human brain research. This paper reviews the most common label-free optical technologies exploited in brain monitoring and their current and potential clinical applications. Label-free tissue monitoring techniques do not require the addition of dyes or molecular contrast agents. The following optical techniques are considered: fNIRS, diffuse correlations spectroscopy (DCS), photoacoustic imaging (PAI) and optical coherence tomography (OCT). Furthermore, wearable optical brain monitoring with the most common applications is discussed.

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“…The SC light was focused into samples with an achromatic lens having a focal length of 30 mm, and the lateral resolution of the SD-OCT was 29 μm, which is similar to that of 1700-nm OCT reported by another group. 20 Figures 4(a) and 4(b) show en-face OCM and OCT images of a pig thyroid gland at a depth of 0.3 mm from the surface. As shown in the result, because both OCM and OCT have the same optical sectioning capabilities, the image contrast in both OCM and OCT imaging was similar.…”

Section: Tissue Imaging With the Developed Sd-ocmmentioning

confidence: 99%

“…[9][10][11][12][13][14][15][16][17][18][19] In recently reported 1700-nm SS-OCT, an imaging depth of 2.6 mm was realized in imaging of living mouse brains. 20 In our group, we have been developing high-axial-resolution 1700-nm OCT by using a supercontinuum (SC) fiber laser source. Based on our OCT system, we also realized 3-D high-resolution timedomain (TD) OCM in the 1700-nm spectral band {the center wavelength is 1730 nm and the spectral bandwidth is 380 nm [full-width at half-maximum (FWHM)]}, which enabled lateral and axial resolutions of 1.3 and 2.8 μm (in tissue).…”

Section: Introductionmentioning

confidence: 99%

High-spatial-resolution deep tissue imaging with spectral-domain optical coherence microscopy in the 1700-nm spectral band

2019

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We present three-dimensional (3-D) highresolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at halfmaximum) in the 1700-nm spectral band. By using lowcoherence interferometry with SC light and a confocal detection scheme, we realized lateral and axial resolutions of 3.4 and 3.8 μm in tissue (n ¼ 1.38), respectively. This is, to the best of our knowledge, the highest 3-D spatial resolution reported among those of Fourier-domain optical coherence imaging techniques in the 1700-nm spectral band. In our SD-OCM, to enhance the imaging depth, a full-range method was implemented, which suppressed the formation of a coherent ghost image and allowed us to set the zero-delay position inside the samples. We demonstrated the 3-D high-resolution imaging capability of 1700-nm SD-OCM through the measurement of an interference signal from a mirror surface and imaging of a single 200-nm polystyrene bead and a pig thyroid gland. Deep tissue imaging at a depth of up to 1.8 mm was also demonstrated. This is the first demonstration of 3-D high-resolution SD-OCM in the 1700-nm spectral band.

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Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation

Cited by 32 publications

References 21 publications

Non-invasive in-vivo 3-D imaging of small animals using spatially filtered enhanced truncated-correlation photothermal coherence tomography

Non-invasive in-vivo 3-D imaging of small animals using spatially filtered enhanced truncated-correlation photothermal coherence tomography

Optics Based Label-Free Techniques and Applications in Brain Monitoring

High-spatial-resolution deep tissue imaging with spectral-domain optical coherence microscopy in the 1700-nm spectral band

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