Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS

Leimgruber, Carolina; Quintar, Amado A.; Sosa, Liliana del Valle; García, Luciana; Figueredo, Mauricio; Maldonado, Cristina A.

doi:10.1002/pros.21322

Cited by 28 publications

(36 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, Cal is downregulated in dedifferentiated vascular SMCs (Shanahan et al, 1993) and after LPS challenge in rat prostate SMCs (Leimgruber et al, 2011) or after treatment of mouse vascular SMC with osteopontin (Gao et al, 2012).…”

Section: Partial Loss Of Contractile Marker Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Partial loss of contractile marker proteins in human testicular peritubular cells in infertility patients

et al. 2012

View full text Add to dashboard Cite

SUMMARYFibrotic remodelling of the testicular tubular wall is common in human male infertility caused by impaired spermatogenesis. We hypothesized that this morphological change bears witness of an underlying fundamentally altered state of the cells building this wall, that is, peritubular smooth muscle-like cells. This could include a loss of the contractile abilities of these cells and thus be a factor in male infertility. Immune cells are increased in the tubular wall in these cases, hence local immune cell-related factors, including a prostaglandin (PG) metabolite may be involved. To explore these points in the human, we used testicular biopsies, in which tubules with normal spermatogenesis and impaired spermatogenesis are next to each other [mixed atrophy (MA)], normal biopsies and cultured human testicular peritubular cells. Proteins essential for contraction, myosin heavy chain (MYH11), calponin (Cal) and relaxation, cGMP-dependent protein kinase 1 (cGKI), were readily detected by immunohistochemistry and were equally distributed in all peritubular cells of biopsies with normal spermatogenesis. In all biopsies, vascular smooth muscle cells also stained and served as important intrinsic controls, which showed that in MA samples when spermatogenesis was impaired, staining was restricted to only few peritubular cells or was absent. When spermatogenesis was normal, regular peritubular staining became obvious. This pattern suggests complex regulatory influences, which in face of the identical systemic hormonal situation in MA patients, are likely caused by the local testicular micromilieu. The PG metabolite 15dPGJ2 may represent such a factor and it reduced Cal protein levels in peritubular cells from patients with/without impaired spermatogenesis. The documented phenotypic switch of peritubular, smooth muscle-like cells in MA patients may impair the abilities of the afflicted seminiferous tubules to contract and relax and must now be considered as a part of the complex events in male infertility.

show abstract

Section: Partial Loss Of Contractile Marker Proteinsmentioning

confidence: 99%

“…Cal is a sensitive marker for the SMC contractile phenotype and exits in two isoforms (Sobue et al, 1999). Basic Cal is specific to SM tissues (Takahashi & Nadal-Ginard, 1991;Strasser et al, 1993) and is known to be regulated by inflammatory factors (Leimgruber et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Partial loss of contractile marker proteins in human testicular peritubular cells in infertility patients

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Stereotypical lesions and intracellular reorganization of the smooth-muscle cells in various diseases indicate common molecular mechanisms of destruction of the ultrastructures and involvement of the leiomyocytes in pathological processes. Smooth-muscle cells constitute the stroma for prostatic glandular epithelium and through paracrine regulation mechanisms modulate the epitheliocyte proliferation, differentiation, and apoptosis and the glandular structures morphogenesis [6,7,13]. The epithelial-stromal interactions play the key role in realization of the hormonal and cytokine regulation of morphogenetic processes in health and neoplastic transformations.…”

Section: Resultsmentioning

confidence: 99%

Role of Sexually-Transmitted Infections in the Structural and Functional Reorganization of the Prostate

et al. 2012

View full text Add to dashboard Cite

Intracellular reorganization of secretory epitheliocytes in the main, intermediate, and periurethral prostatic glands was studied in chronic prostatitis under conditions of sexually-transmitted infections. The destructive and autophagic processes in the secretory epitheliocytes were stimulated by persistence of microorganisms, Mycoplasmataceae (mainly mycoplasmas and ureaplasmas) and Chlamydia trachomatis, in the prostatic terminal compartments, epithelial layer, and epitheliocytes. Significant intracellular reorganization of smooth-muscle cells was found: focal destruction of ultrastructures (mainly in the perinuclear zone) and lythic changes in the myofilaments (focal and diffuse).

show abstract

“…It was suggested in the same study that prostatic smooth muscle cells become secretory cells in response to E. coli. Additionally, Leimgruber et al described a dedifferentiation process of smooth muscle cells after bacterial LPS treatment, with a loss of cytoskeleton molecules that characterized contractile phenotype (8). In another recent study, it was shown that inflammation caused the loss of smooth muscle cells around prostate ducts (24).…”

Section: Discussionmentioning

confidence: 99%

“…Some recent papers showed that prostatitis elicited by E. coli caused an uncontrolled growth in the prostate gland at the early stages of inflammation (8,9). On the other hand, in previous studies, it was shown that uropathogenic E. coli or bacterial lipopolysaccharide (LPS) treatment causes hypocontractility in various tissues, including vessels, the urinary bladder, the corpus cavernosum, and the vas deferens (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Effect of melatonin on contractile activity in intraurethrally instilled E. coli-induced acute prostatitis rat model

Huseynov¹,

Eser²,

Aridogan³

et al. 2013

Turk J Med Sci

View full text Add to dashboard Cite

In the present study, some new 2-(pyridin-2-yl)thieno [2,3-d ]pyrimidin-4(3 H)-ones derivatives (IIa-o) were synthesized. The target compounds (IIa-o) were synthesized through the acid catalyzed condensation of 2-cyano, 3-cyano, and 4-cyano-pyridines with various 2-amino-3-carbethoxythiophenes (Ia-e). All thiophene derivatives were synthesized by Gewald reaction. The structures of the newly synthesized compounds were confirmed by UV-Visible, FT-IR, 1 H-NMR, and mass spectral studies. All synthesized compounds were evaluated for their antimicrobial activity against various gram-positive and gram-negative bacterial and fungal strains. Amongst the synthesized compounds IIa, IIb, IId, IIe, and IIm were found to be active.

show abstract

Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS

Cited by 28 publications

References 29 publications

Partial loss of contractile marker proteins in human testicular peritubular cells in infertility patients

Partial loss of contractile marker proteins in human testicular peritubular cells in infertility patients

Role of Sexually-Transmitted Infections in the Structural and Functional Reorganization of the Prostate

Effect of melatonin on contractile activity in intraurethrally instilled E. coli-induced acute prostatitis rat model

Contact Info

Product

Resources

About