Dedifferentiated variants of a rat hepatoma: Reversion analysis

Deschatrette, Jean; Moore, Emma; Dubois, Monique; Weiss, Mary C.

doi:10.1016/0092-8674(80)90095-1

Cited by 124 publications

(68 citation statements)

References 24 publications

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“…Moreover, it has been possible to select, in glucose-free medium, revertants of FaoflC2 cells but not of H5. These revertants show restoration of the entire group of liver-specific functions analyzed (22), and the clone analyzed for repeat length, C2-Rev7, shows the same probability distribution as FaoflC2 cells (not shown, but see Table 1). Thus, FaoflC2 differs less than H5 and p4 from the well-differentiated clones not only in repeat length but also in biological properties.…”

Section: Methodsmentioning

confidence: 98%

Chromatin repeat length correlates with phenotypic expression in hepatoma cells, their dedifferentiated variants, and somatic hybrids.

Sperling¹,

Weiss²

1980

Proc. Natl. Acad. Sci. U.S.A.

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Section: Methodsmentioning

confidence: 98%

Chromatin repeat length correlates with phenotypic expression in hepatoma cells, their dedifferentiated variants, and somatic hybrids.

Sperling¹,

Weiss²

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…These cells probably represent mutants as their phenotype has been stable in culture for 13 years and they arise at a frequency of -10-7. The lack of expression of most of the gluconeogenic enzymes in the C2 cells allowed the selection of revertants by growth in glucose-free media (Deschatrette et al 1980). The revertants (Rev7 cells), which occur at a frequency of 10 -9 , express nearly all of the liver-specific proteins and are morphologically similar to the original differentiated cells (Deschatrette et al 1980), perhaps because cells need several hepatocyte-specific proteins to perform gluconeogenesis and grow in media without glucose.…”

mentioning

confidence: 99%

“…The lack of expression of most of the gluconeogenic enzymes in the C2 cells allowed the selection of revertants by growth in glucose-free media (Deschatrette et al 1980). The revertants (Rev7 cells), which occur at a frequency of 10 -9 , express nearly all of the liver-specific proteins and are morphologically similar to the original differentiated cells (Deschatrette et al 1980), perhaps because cells need several hepatocyte-specific proteins to perform gluconeogenesis and grow in media without glucose. Although the original differentiated Fao cells expressed the 88-kD form of HNF-1 found in liver extracts, the C2 cells expressed a 68-kD variant form of HNF-1 (vHNF-1) that exhibited DNA sequence specificity identical to the original HNF-1 protein Cereghini et al 1988).…”

mentioning

confidence: 99%

HNF-1 alpha and HNF-1 beta (vHNF-1) share dimerization and homeo domains, but not activation domains, and form heterodimers in vitro.

Mendel¹,

Hansen²,

Graves³

et al. 1991

Genes Dev.

294

218

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“…According to Deschatrette et al [6] H56 cells are candidates for the regulatory 'mutant', whose defect originates in the inappropriate expression of mechanisms which control the orderly temporal expression of tissue-specific functions during development. One can hypothesise that among spots present only in H56 cells can be found negative regulatory protein(s) responsible for the dedifferentiated state of this clone.…”

Section: Resultsmentioning

confidence: 99%

“…They were isolated and characterized by Deschatrette and Weiss [4,6]. Both cell lines are sensitive to dexamethasone (Dex) (growth sensitivity).…”

Section: Cell Lines Media Culture Conditionsmentioning

confidence: 99%

Protein synthesis in differentiated and dedifferentiated hepatoma cell lines

Bösze¹,

Arányi²,

Venetianer³

1983

European Journal of Biochemistry

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The pattern of protein synthesis in hepatoma cell clones was analysed by two-dimensional separation of [3 5S]methionine-iabelled proteins. The clones were derived from the differentiated Reuber H 35 hepatoma and showed differences in the expression of a number of liver-specific functions and the resistance to the growthinhibitory effect of glucocorticoids.Five protein spots were observed in the extracts of the differentiated Faza 967 cells that were absent from the electrophoretogram of the dedifferentiated H56 cells. This clone, on the other hand, displayed six spots absent from Faza 967 cells. The growth of both Faza 967 and H56 cells was strongly inhibited by 1 pM dexamethasone.The dexamethasone-resistant clone 2, a dedifferentiated derivative of Faza 967 cells, synthesized two polypeptides that were not present in Faza 967 or H 56 cells and produced four polypeptides at a lower level than Faza 967 cells. The examination of the short-term effect of dexamethasone on protein synthesis in Faza 967 cells revealed nine induced and one repressed protein spots, which appeared to be in good agreement with earlier published data. It is concluded that dedifferentiation, although bringing about marked changes in certain liver-specific functions, such as enzyme activities or protein secretion, affects only a relatively small fraction of the genes expressed.Hormones play an essential role in gene regulation in multicellular organisms by eliciting specific responses from target cells. It is well known that glucocorticoid hormones have multiple effects in the development and maintenance of the differentiated state of cells [l]. Different cell lines have different phenotypes with respect to their hormone responsiveness. Hepatoma cells express a wide range of liverspecific functions, some of which are under glucocorticoid regulation. Cell lines derived from Reuber H35 rat hepatoma [2,3] express numerous functions of the hepatocytes of the adult liver (e.g. production and secretion of serum albumin, basal activity and inducibility of tyrosine aminotransferase and alanine aminotransferase by glucocorticoids, the liverspecific isozymes of aldolase and alcohol dehydrogenase and the activity of gluconeogenetic enzymes fructose-I ,6-diphosphatase and phosphoenolpyruvate carboxykinase). The hepatoma cells expressing all the above functions will be termed 'well differentiated' hepatomas, while the dedifferentiated ones fail to express the whole group of differentiated functions, and the partially dedifferentiated ones express only some of them [4 -61. The growth of the well-differentiated (Faza 967) and the spontaneously dedifferentiated variants (H56) of the Reuber H35 hepatomas are inhibited by glucocorticoids. We isolated a series of stable dexamethasoneresistant variants from the hormone-sensitive Faza 967 clone [7]. As we reported recently the expression of almost all the differentiated functions decreased or disappeared in these hormone-resistant cell lines during long-term cultivation, in contrast to the sensitive cells, where...

show abstract

Dedifferentiated variants of a rat hepatoma: Reversion analysis

Cited by 124 publications

References 24 publications

Chromatin repeat length correlates with phenotypic expression in hepatoma cells, their dedifferentiated variants, and somatic hybrids.

Chromatin repeat length correlates with phenotypic expression in hepatoma cells, their dedifferentiated variants, and somatic hybrids.

HNF-1 alpha and HNF-1 beta (vHNF-1) share dimerization and homeo domains, but not activation domains, and form heterodimers in vitro.

Protein synthesis in differentiated and dedifferentiated hepatoma cell lines

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