Dectin-1-mediated Signaling Leads to Characteristic Gene Expressions and Cytokine Secretion via Spleen Tyrosine Kinase (Syk) in Rat Mast Cells

Abstract

Background: ␤-Glucan receptor Dectin-1 in dendritic cells and macrophages plays important roles in antifungal immunity. Results: Dectin-1 is expressed in rat mast cells, and its tyrosine phosphorylation induces characteristic gene expression of transcription factors and cytokines through protein-tyrosine kinase Syk. Conclusion: Dectin-1 functions in rat mast cells. Significance: Dectin-1-mediated signaling in mast cells may contribute to antifungal immunity.

STAT1 is essential for the inhibition of hepatitis C virus replication by interferon-λ but not by interferon-α

“…Sense-strand DNA was generated from 200 ng of total RNA, fragmented, and labeled using a GeneChip WT Plus Reagent Kit (Affymetrix). Hybridization and scanning were performed as described previously48, except that GeneChip HuGene 2.0 ST Arrays (Affymetrix) were used. Hierarchical clustering (uncentered correlation) was performed using Subio Platform version 1.19 (Subio).…”

Association of C-Type Lectin Mincle with FcεRIβγ Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk

“…Engagement of Dectin-1 causes Syk activation, leading to the secretion of cytokines and chemokines from RBL-2H3 cells16. In addition to Dectin-1, we found mRNA expression of another member of the C-type lectins, Mincle, in RBL-2H3 cells by RT-PCR (Supplementary Fig.…”

“…One possible explanation for the difference is that the ITAM of FcεRIγ associated with FcεRIβ may efficiently activate Syk compared with the Dectin-1 hemITAM. In fact, ligand-induced tyrosine phosphorylation of cellular proteins, including the hemITAM of Dectin-1 by itself, reaches the maximum level at around 40 min after stimulation16, but it could be observed within 10 min by stimulation of Mincle (Fig. 2).…”

“…Recently, we showed that activation of Dectin-1 leads to up-regulation of characteristic mast cell genes in ERK- and NFAT-dependent manners16. These observations prompted us to examine whether the Mincle-mediated Syk-PLCγ2 signaling pathway induces the activation of ERK and NFAT in RBL-2H3 cells.…”

“…Tyrosine phosphorylation of hemITAM is critical for activation of Syk, leading to elevation of transcriptional activities to enhance the production of various cytokines and chemokines81415. In addition, we have shown that Dectin-1 expressed on RBL-2H3 cells stimulates Syk-mediated gene expression and secretion of cytokines and chemokines16.…”