Decrement in Cellular Iron and Reactive Oxygen Species, and Improvement of Insulin Secretion in a Pancreatic Cell Line Using Green Tea Extract

Koonyosying, Pimpisid; Uthaipibull, Chairat; Fucharoen, Suthat; Koumoutsea, Evangelia Vlachodimitropoulou; Porter, John B.; Srichairatanakool, Somdet

doi:10.1097/mpa.0000000000001320

Cited by 22 publications

(31 citation statements)

References 51 publications

(58 reference statements)

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“…Surprisingly, we demonstrate that treatment of GTE (1-10 μM EGCG equivalent) decreased cellular iron approximately 45% and ROS level in a concentration-dependent manner in ironloaded pancreatic cell line (RINm5F) when compared with control cells. Secretory insulin level was nearly 2.5-fold times the highest safe concentration of the GTE [36]. The results imply that catechin-rich GT would indeed be an effective drink to remove iron, decrease ROS, and improve pancreatic cell function thereby increasing insulin production, leading to the amelioration of diabetic complications in thalassemia patients with iron overload.…”

Section: Green Teamentioning

confidence: 81%

“…Moreover, the β-cell pancreatic function and adrenal cortical function were depressed in the severely iron-loaded. Recently, Koonyosying and colleagues have demonstrated green tea extract could reduce cellular the levels of iron and ROS and increase insulin secretion in concentration-dependent manner in iron-loaded pancreatic cell line (RINm5F), indicating the amelioration of oxidative stress and endocrinal improvement of pancreatic β-cells [36]. They also found that eltrombopag, which is a thrombopoietin receptor agonist and potent metal ion-chelating agent, efficiently decreased cellular levels of iron and ROS from cultured HuH7, H9C2, and RINm5F cells and restored insulin secretion from iron-loaded RINm5F cells [37].…”

Section: Tissue Iron Depositionmentioning

confidence: 99%

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Diet-Related Thalassemia Associated with Iron Overload

Srichairatanakool¹,

Koonyosying²,

Fucharoen³

2020

Personalized Medicine, in Relation to Redox State, Diet and Lifestyle

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Thalassemia is an inherited disease caused by the genetic disorder of α-and β-globin genes, resulting in ineffective erythropoiesis and chronic anemia. Transfusion-dependent β-thalassemia patients require red cell transfusion to maintain their blood hemoglobin level in the normal range, whereas non-transfusion-dependent thalassemia patients increase duodenal absorption of dietary iron in an attempt to accelerate erythropoiesis. These changes give rise to iron overload, oxidative stress, organ dysfunction, and other complications. Effective iron chelators are necessary to achieve negative iron balance and to relieve such complications associated with iron overload. Some pharmaceuticals such as hydroxyurea, N-acetylcysteine, ascorbic acid, vitamin E, and glutathione are also given to thalassemia patients in order to overcome oxidative cell and tissue damage and to generate a better quality of life. Interestingly, functional natural products (such as mango, tea, caffeine, and curcumin), vegetables, and cereal (e.g., rice) are helpful for their health-providing properties by supplementing the endogenous antioxidant defensive power in the body. Natural products exhibit many pharmacological activities, but they are safer if used in the traditional manner.

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Section: Green Teamentioning

confidence: 81%

Section: Tissue Iron Depositionmentioning

confidence: 99%

Diet-Related Thalassemia Associated with Iron Overload

Srichairatanakool¹,

Koonyosying²,

Fucharoen³

2020

Personalized Medicine, in Relation to Redox State, Diet and Lifestyle

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“…ROS induced by FAC, which finally cause β-cell damage. FAC was previously used to induce the excess intracellular iron and caused increasing of ROS affected insulin secretion in Rat insulinoma pancreatic β-cells (RINm5F) as a model of pancreatic iron overload in β-thalassemia patients (Koonyosying et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Combination of ferric ammonium citrate with cytokines involved in apoptosis and insulin secretion of human pancreatic beta cells related to diabetes in thalassemia

Rattanaporn

Tongsima

Mandrup‐Poulsen

et al. 2020

PeerJ

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Background Diabetes mellitus (DM) is a common complication found in β-thalassemia patients. The mechanism of DM in β-thalassemia patients is still unclear, but it could be from an iron overload and increase of some cytokines, such as interleukin1-β (IL-1β) and tumor necrosis factor-α (TNF-α). The objective of this study was to study the effect of interaction between ferric ammonium citrate (FAC) and cytokines, IL-1β and TNF-α, on 1.1B4 human pancreatic β-cell line. Methods The effect of the combination of FAC and cytokines on cell viability was studied by MTT assay. Insulin secretion was assessed by the enzyme-linked immunosorbent assay (ELISA). The reactive oxygen species (ROS) and cell apoptosis in normal and high glucose condition were determined by flow cytometer. In addition, gene expression of apoptosis, antioxidant; glutathione peroxidase 1 (GPX1) and superoxide dismutase 2 (SOD2), and insulin secretory function were studied by real-time polymerase chain reaction (Real-time PCR). Results The findings revealed that FAC exposure resulted in the decrease of cell viability and insulin-release, and the induction of ROS and apoptosis in pancreatic cells. Interestingly, a combination of FAC and cytokines had an additive effect on SOD2 antioxidants’ genes expression and endoplasmic reticulum (ER) stress. In addition, it reduced the insulin secretion genes expression; insulin (INS), glucose kinase (GCK), protein convertase 1 (PSCK1), and protein convertase 2 (PSCK2). Moreover, the highest ROS and the lowest insulin secretion were found in FAC combined with IL-1β and TNF-α in the high-glucose condition of human pancreatic beta cell, which could be involved in the mechanism of DM development in β-thalassemia patients.

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“…Insulin deficiency and IRes have been described in ß-thalassemia patients with iron overload, which is probably a secondary effect of a diminished pancreatic beta cell function. The incubation of iron-loaded rat insulinoma pancreatic β-cells with a GTE (2.29 µg EGCG equivalent) increased insulin secretion levels 2.5-fold and decreased cellular levels of iron and reactive oxygen species (ROS) [ 33 ].…”

Section: Antidiabetic Effects Of Flavan-3-ols: In Vitro and In Vivmentioning

confidence: 99%

Antidiabetic Effects of Flavan-3-ols and Their Microbial Metabolites

2020

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Diet is one of the pillars in the prevention and management of diabetes mellitus. Particularly, eating patterns characterized by a high consumption of foods such as fruits or vegetables and beverages such as coffee and tea could influence the development and progression of type 2 diabetes. Flavonoids, whose intake has been inversely associated with numerous negative health outcomes in the last few years, are a common constituent of these food items. Therefore, they could contribute to the observed positive effects of certain dietary habits in individuals with type 2 diabetes. Of all the different flavonoid subclasses, flavan-3-ols are consumed the most in the European region. However, a large proportion of the ingested flavan-3-ols is not absorbed. Therefore, the flavan-3-ols enter the large intestine where they become available to the colonic bacteria and are metabolized by the microbiota. For this reason, in addition to the parent compounds, the colonic metabolites of flavan-3-ols could take part in the prevention and management of diabetes. The aim of this review is to present the available literature on the effect of both the parent flavan-3-ol compounds found in different food sources as well as the specific microbial metabolites of diabetes in order to better understand their potential role in the prevention and treatment of the disease.

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Decrement in Cellular Iron and Reactive Oxygen Species, and Improvement of Insulin Secretion in a Pancreatic Cell Line Using Green Tea Extract

Abstract: Supplemental digital content is available in the text.

Cited by 22 publications

References 51 publications

Diet-Related Thalassemia Associated with Iron Overload

Diet-Related Thalassemia Associated with Iron Overload

Combination of ferric ammonium citrate with cytokines involved in apoptosis and insulin secretion of human pancreatic beta cells related to diabetes in thalassemia

Antidiabetic Effects of Flavan-3-ols and Their Microbial Metabolites

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