Low-grade weirs placed within agricultural drainage ditches in the Lower Mississippi Alluvial Valley can be used as a management practice to enhance nitrogen removal. The addition of organic carbon amendments within ditches that contain weirs could further increase nitrogen removal. Through repeated trials, changes in NO − 3 -N concentration between inflow and outflow were variable in the ditch without weirs, while only decreases in concentration were observed in ditches with weirs. Significant differences in NO − 3 -N concentrations were observed between treatments, with greater removal of NO − 3 -N observed in dissolved organic carbon treatments compared to control and particulate organic carbon treatments. At medium-and high-flow rates, respectively, dissolved organic carbon treatments resulted in greater NO − 3 -N concentration decreases of 31.6% and 27.1% compared to 19% and 11.6% in particulate organic carbon treatments and 18.6% and 17.2% in control treatments. Significant effects of weirs and sampling date on nirS, nirK, nosZ, and 16S rRNA gene abundances were observed. Observed increases in NO − 3 -N removal with organic carbon amendments, provides support for continued investigation on improving the efficacy of organic carbon amendments as a best management practice for NO − 3 -N removal in agricultural drainage ditches.
• Practitioner points• Dissolved organic carbon amendments increased nitrate-nitrogen removal. • Only decreases in nitrate-nitrogen concentration were observed in ditches with weirs. • Increasing flow rate did not affect nitrate-nitrogen removal. • Abundance of denitrification-performing microbes likely did not affect N removal. • Lack of anaerobic soil conditions and short residence time reduced nitrate-N removal.