Decreasing arsenic accumulation in rice by overexpressing <i>Os<scp>NIP</scp>1;1</i> and <i>Os<scp>NIP</scp>3;3</i> through disrupting arsenite radial transport in roots

Sun, Shengwei; Chen, Yi; Che, Jianfei; Konishi, Noriyuki; Tang, Zhong; Miller, Anthony J.; Feng, Jian; Zhao, Fang‐Jie

doi:10.1111/nph.15190

Cited by 126 publications

(53 citation statements)

References 60 publications

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“…Germinated seedlings were transferred to a black net, floating on tap water for 1 week. Seedlings were then transferred to a 16-L plastic pot, containing half of Kimura B solution (Sun et al, 2018). The pH of this solution was adjusted to 5.6, and the nutrient solution was renewed every 2 days.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice

Chang

Huang

Yamaji

et al. 2020

Plant Cell & Environment

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243

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Rice is a major dietary source of the toxic metal, cadmium (Cd). Previous studies reported that the rice transporter, OsNRAMP1, (Natural resistance‐associated macrophage protein 1) could transport iron (Fe), Cd and arsenic (As) in heterologous yeast assays. However, the in planta function of OsNRAMP1 remains unknown. Here, we showed that OsNRAMP1 was able to transport Cd and manganese (Mn) when expressed in yeast, but did not transport Fe or As. OsNRAMP1 was mainly expressed in roots and leaves and encoded a plasma membrane‐localized protein. OsNRAMP1 expression was induced by Cd treatment and Fe deficiency. Immunostaining showed that OsNRAMP1 was localized in all root cells, except the central vasculature, and in leaf mesophyll cells. The knockout of OsNRAMP1 resulted in significant decreases in root uptake of Cd and Mn and their accumulation in rice shoots and grains, and increased sensitivity to Mn deficiency. The knockout of OsNRAMP1 had smaller effects on Cd and Mn uptake than knockout of OsNRAMP5, while knockout of both genes resulted in large decreases in the uptake of the two metals. Taken together, OsNRAMP1 contributes significantly to the uptake of Mn and Cd in rice, and the functions of OsNRAMP1 and OsNRAMP5 are similar but not redundant.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice

Chang

Huang

Yamaji

et al. 2020

Plant Cell & Environment

Self Cite

243

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“…More As accumulated in the roots can be due to over-expression of transcription factor OsARM1 (ARSENITE-RESPONSIVE MYB1), which leads to the down-regulation of expression of OsLsi2 and NRAMP1 transporters that are responsible for xylem loading and transport from root to shoot [118]. Over-expression of transporters OsNIP1;1 and OsNIP3;3 localized on the plasma membrane can restrict As loading into xylem and decreased As accumulation in shoots (41-56%) [119]. Furthermore, disruption of inositol transporters (AtINT2 and AtINT4) can decrease As concentrations in phloem and subsequently decrease As content in leaves [120].…”

Section: As Concentrations In Soil/fly Ash/plantmentioning

confidence: 99%

Phytoremediation Potential, Photosynthetic and Antioxidant Response to Arsenic-Induced Stress of Dactylis glomerata L. Sown on Fly Ash Deposits

Gajić

Djurdjević

Kostić

et al. 2020

Plants

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Arsenic (As) from coal fly ash can be released into soil/groundwater, presenting a global threat to the environment and human health. To overcome this environmental problem, phytoremediation represents an urgent need, providing ‘green’ cleanup of contaminated lands. The present study focused on As concentrations in fly ash and plants, evaluation of phytoremediation potential of Dactylis glomerata sown on fly ash deposits together with its photosynthetic activity, and oxidative and antioxidative response to As stress. Field research was carried out on fly ash deposits at the thermal power plant “Nikola Tesla”, Obrenovac (TENT-A, Serbia) and the control site. Fly ash is characterized by alkaline pH reactions, small amounts of organic matter, a large amount of available phosphate, and total and available As concentrations. Results in this study indicate that phosphate application can ameliorate As toxicity, uptake and root-shoot transport. Furthermore, D. glomerata can be considered as good As phytostabilizator, because it retains more As in roots than in leaves. Excess As in leaves decreases photosynthetic efficiency (Fv/Fm) and concentrations of chlorophylls, carotenoids, and anthocyanins, whereas high content of malondialdehyde (MDA) can be a signal for biosynthesis phenolics and ascorbic acid, providing cellular redox homeostasis and recovery of photosystem II (PSII) photochemistry. In the roots, low oxidative stress under high concentrations of As is related to intense antioxidant biosynthesis. Taken together, the results in this study indicate a high adaptive potential of D. glomerata to As stress. These findings may suggest that physiological and metabolic tools can be used as a way forward in the ‘real field’ scenario, phytomanagement of fly ash and ecosystem services providing sustainable phytoremediation of As-contaminated sites around the globe.

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“…In addition, AsIII efflux to the external medium is a vital way of As detoxification in plants. Inside plant cells, AsIII may be detoxified by complexation with phytochelatins (PCs), followed by the accumulation of AsIII-PC complexes in vacuoles through OsABCC1 transporters [69]. OsABCC1 is one of the ATP-binding cassette (ABC) transporters.…”

Section: Phytotoxicity Of Arsenic and Arsenic Detoxification Mechanismentioning

confidence: 99%

Arsenic Uptake and Accumulation Mechanisms in Rice Species

Abedi

Mojiri

2020

Plants

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Rice consumption is a source of arsenic (As) exposure, which poses serious health risks. In this study, the accumulation of As in rice was studied. Research shows that As accumulation in rice in Taiwan and Bangladesh is higher than that in other countries. In addition, the critical factors influencing the uptake of As into rice crops are defined. Furthermore, determining the feasibility of using effective ways to reduce the accumulation of As in rice was studied. AsV and AsIII are transported to the root through phosphate transporters and nodulin 26-like intrinsic channels. The silicic acid transporter may have a vital role in the entry of methylated As, dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA), into the root. Amongst As species, DMA(V) is particularly mobile in plants and can easily transfer from root to shoot. The OsPTR7 gene has a key role in moving DMA in the xylem or phloem. Soil properties can affect the uptake of As by plants. An increase in organic matter and in the concentrations of sulphur, iron, and manganese reduces the uptake of As by plants. Amongst the agronomic strategies in diminishing the uptake and accumulation of As in rice, using microalgae and bacteria is the most efficient.

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Decreasing arsenic accumulation in rice by overexpressing OsNIP1;1 and OsNIP3;3 through disrupting arsenite radial transport in roots

Cited by 126 publications

References 60 publications

OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice

OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice

Phytoremediation Potential, Photosynthetic and Antioxidant Response to Arsenic-Induced Stress of Dactylis glomerata L. Sown on Fly Ash Deposits

Arsenic Uptake and Accumulation Mechanisms in Rice Species

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