Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes

Zamproni, Laura N.; Teixeira, Daniela; Alliegro, Amanda Arnaut; Maugéri, Ieda Longo; Rieux, Anne des; Porcionatto, Marimélia

doi:10.1016/j.neuro.2019.10.001

Cited by 7 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed that CHI negatively affected neuronal maturation but instead allowed the development of astrocytes at a higher ratio (Figure 4M). Similar outcomes were observed in previous CNS reports where CHI nanoparticles as a drug delivery system reduced neuronal viability and neurite extension while impairing neural stem cell migration [80]. This effect could be attributed to the high charge density of CHI, creating a proneuroinflammatory environment that damages neurons but supports glial cells [81].…”

Section: Discussionsupporting

confidence: 86%

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

da Silva,

Bobotis,

Correia

et al. 2023

IJMS

View full text Add to dashboard Cite

Tissue engineering for spinal cord injury (SCI) remains a complex and challenging task. Biomaterial scaffolds have been suggested as a potential solution for supporting cell survival and differentiation at the injury site. However, different biomaterials display multiple properties that significantly impact neural tissue at a cellular level. Here, we evaluated the behavior of different cell lines seeded on chitosan (CHI), poly (ε-caprolactone) (PCL), and poly (L-lactic acid) (PLLA) scaffolds. We demonstrated that the surface properties of a material play a crucial role in cell morphology and differentiation. While the direct contact of a polymer with the cells did not cause cytotoxicity or inhibit the spread of neural progenitor cells derived from neurospheres (NPCdn), neonatal rat spinal cord cells (SCC) and NPCdn only attached and matured on PCL and PLLA surfaces. Scanning electron microscopy and computational analysis suggested that cells attached to the material’s surface emerged into distinct morphological populations. Flow cytometry revealed a higher differentiation of neural progenitor cells derived from human induced pluripotent stem cells (hiPSC-NPC) into glial cells on all biomaterials. Immunofluorescence assays demonstrated that PCL and PLLA guided neuronal differentiation and network development in SCC. Our data emphasize the importance of selecting appropriate biomaterials for tissue engineering in SCI treatment.

show abstract

Section: Discussionsupporting

confidence: 86%

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

da Silva,

Bobotis,

Correia

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Whatever the type of cell death, these guidelines describe the rearrangements that cytoskeletal dynamics could undergo (Kroemer et al, 2008;Galluzzi et al, 2018;Ren et al, 2021). Although the absence of cytotoxicity is a general feature of CS-NCs, in some specific systems, such as tumoral cells, death pathways are triggered (Venkatesan et al, 2011;Taranejoo et al, 2016;Zamproni et al, 2020) (Fig. 1).…”

Section: Chitosan-based Ncs Uptake and Its Impact On The Cytoskeleton Dynamicsmentioning

confidence: 99%

Impact of chitosan-based nanocarriers on cytoskeleton dynamics: Current status and challenges

Santo¹,

Alaimo²,

RUBIO³

et al. 2022

Biocell

View full text Add to dashboard Cite

Chitosan-based nanocarriers (CS-NCs) show a promising role in improving drugs and bioactive compounds delivery for therapy. However, the effects exerted by CS-NCs at the cellular level, including their recognition and uptake, have not been fully investigated yet. Many factors, including size, shape, concentration, and surface chemistry of CS-NCs, play an important role in determining the types of intracellular signals triggered. The mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside cells. In the present work, we discuss the effects induced by CS-NCs per se on the cytoskeleton, a key component in cell architecture and physiology. The focus of this report is made on tumoral and normal biological models in which CS-NCs could differentially affect the cell cytoskeleton. The recent years reports regarding the impact of CS-NCs on cytoskeleton dynamics and the current techniques for its evaluation are summarized and discussed. Understanding mechanisms underlying cytoskeletal impact after cell exposure to CS-NCs is critical for the design of safest value-added formulations in the biomedical field. Furthermore, this revision points out some interesting aspects of cytoskeletal changes and cell death encompassing anti-tumoral effects.

show abstract

“…Y. Chen was the first to fabricate and entrap an arginine-rich hexapeptide into CS-DS nanoparticles [22]. This increased the popularity of biopolymer-based nanoparticle research and led to further developments in the field such as the encapsulation of insulin into CS-based nanoparticles [23,24].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Biopolymer Based Nanoparticles for the Entrapment of Chromium and Iron Supplements

2020

View full text Add to dashboard Cite

The objective of this study was to encapsulate iron and chromium into novel nanoparticles formulated using chitosan (CS), dextran sulfate (DS) and whey protein isolate (WPI) for oral drug delivery. Empty and loaded CS-DS nanoparticles were prepared via complex coacervation whilst whey protein nanocarriers were produced by a modified thermal processing method using chitosan. The physiochemical properties of the particles were characterized to determine the effects of formulation variables, including biopolymer ratio on particle size and zeta potential. Permeability studies were also undertaken on the most stable whey protein–iron nanoparticles by measuring Caco-2 ferritin formation. A particle size analysis revealed that the majority of samples were sub-micron sized, ranging from 420–2400 nm for CS-DS particles and 220–1000 nm for WPI-CS samples. As expected, a higher chitosan concentration conferred a 17% more positive zeta potential on chromium-entrapped WPI nanoparticles, whilst a higher dextran volume decreased the size of CS-DS nanoparticles by 32%. The addition of iron also caused a significant increase in size for all samples, as seen where the loaded WPI samples were 296 nm larger than the empty particles. Caco-2 iron absorption revealed that one formulation, which had the lowest particle size (226 ± 10 nm), caused a 64% greater iron absorption compared to the ferrous sulfate standard. This study describes, for the first time, the novel design of chromium- and iron-entrapped nanoparticles, which could act as novel systems for oral drug delivery.

show abstract

Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes

Cited by 7 publications

References 34 publications

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

Impact of chitosan-based nanocarriers on cytoskeleton dynamics: Current status and challenges

Fabrication of Biopolymer Based Nanoparticles for the Entrapment of Chromium and Iron Supplements

Contact Info

Product

Resources

About