Decreased UDP‐glucuronic acid in rat liver after ether narcosis

Eriksson, Göran; Stråth, Daniel

doi:10.1016/0014-5793(81)80049-x

Cited by 30 publications

(3 citation statements)

References 20 publications

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“…Experiments with laboratory animals to study liver structure and function traditionally have been carried out with barbiturates or diethylether anesthesia. Certain effects of these anesthetics on the liver have been well documented (Bolender and Weibel, 1973;Eriksson and Strath, 1981;Kuriyama et al, 1969;Vermeulen et al, 1983;Watkins et al, 1984).…”

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confidence: 99%

Isoflurane as an anesthetic for experimental animal surgery

et al. 1987

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Isoflurane is an inhalational anesthetic agent associated with no known hepatic toxicity. Despite this fact, isoflurane has not been widely utilized as an anesthetic agent in studies of liver structure and function in experimental animals. For this reason, livers from rats treated with pentobarbital or diethylether were compared to those from rats treated with isoflurane to determine differences in biochemical and morphologic parameters. Liver from pentobarbital-treated rats showed a significant decline in glutathione-S-transferase activity compared to liver from isoflurane/O2 or ether-treated rats. Liver microsomes from isoflurane/O2-treated rats retained more cytochrome-C(P450)-reductase activity than did those from pentobarbital-treated, ether-treated, or decapitated rats. Despite these biochemical alterations, morphometric analysis of liver from isoflurane/O2 and pentobarbital-treated rats showed no quantitative or qualitative differences in liver structure or organelle volume densities. Neither were differences detected in uptake and distribution of 125I-epidermal growth factor when analyzed by electron microscopic autoradiography. These data show that isoflurane with supplemental O2 has no effects on hepatic structure and fewer effects on hepatic function than other anesthetics and may be a better experimental anesthetic than any currently in use.

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confidence: 99%

Isoflurane as an anesthetic for experimental animal surgery

et al. 1987

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“…A significant decrease in the uridinediphosphate-glucuronic acid (UDP-GlcUA) concentration without a similar change in ATP/ADP/AMP ratio has been reported during anaesthesia with ether [Eriksson and Strâth, 1981], Ether may thus decrease the concentration of activated co-substrate for the glucuronyl transferase reaction and it could be speculated that similar effects on the formation of PAPS might occur during ether anaesthesia. The concept of co-substrate de pletion is consistent with the observation of a normal rate of sulphanilamide acetylation in rat hepatocytes in the presence of ether, since the metabolism of ether to acetaldehyde has been shown to take place in several species [Peters and Shourthouse, 1975;Aune et al, 1978;Slowed et al, 1981], with the possibil ity of further metabolism to acetate and CoA, the co-substrate of acetylation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Diethylether on the Formation of Paracetamol Sulphate and Glucuronide in Isolated Rat Hepatocytes

et al. 1984

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Diethylether has previously been shown to inhibit several pathways of drug metabolism, including conjugation of paracetamol in isolated rat hepatocytes. Since overall paracetamol conjugation consists of pathways of different subcellular localization (cytosolar sulphation and microsomal glucuronidation) the response of both pathways to diethylether was tested. The elimination of paracetamol (160 µmol/l, initial concentration) and the formation of paracetamol sulphate and glucuronide were measured (high-performance liquid chromatography) in suspensions of isolated rat hepatocytes from fasted and fed animals over 1 h in the absence and presence of diethylether (30 mmol/l). Approximately 90% of the paracetamol elimination was by sulphation and nearly 10% by glucuronidation both in the controls and in the presence of ether. The overall disposition of paracetamol and the formation of sulphate were both reduced by about 50% in the presence of ether compared to the controls while the formation of glucuronide was reduced by 70%. The results were not influenced by the nutritional state of the animals before sacrifice. It is concluded that the inhibitory effect of ether on total paracetamol metabolism was mainly caused by reduced sulphation. Since microsomal glucuronidation was also inhibited by ether, both cytosolar and microsomal enzyme systems were sensitive to diethylether.

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“…In conclusion, snips possess the often important cell-cell interactions of perfused tissue and slices, and the high reproducibility of cell suspensions. Their preparation is simpler than these, and incurs neither digestion at cell interfaces, which may alter cell membranes (see Dutton, 1980), nor anaesthesia, which may also affect cell membranes and drastically change nucleotide concentrations (Eriksson & Strath, 1981). Snips are particularly suitable for exploratory and topological work, or for studies such as those on perinatal development, where the need for large sample pools and the use of small fragile livers precludes perfusion and discourages 'slicing', and where age-dependent conditions for isolating minimally damaged cells complicate the use of hepatocytes.…”

Section: Comparison With Other Intact-cell Preparationsmentioning

confidence: 99%

Liver snips. A simple, rapid and reproducible method for studying metabolism in small fragments of tissue, as applied to glucuronidation in rat liver

Pollard¹,

Dutton²

1982

Biochemical Journal

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A simple, rapid method is described of preparing intact cells as small (about 2mm) pieces of organized tissue capable of performing synthetic metabolic functions. It has been applied to the study of glucuronidation in rat liver. In this process, snips appear less damaged, more versatile and more active than tissue slices and yield results of reproducibility comparable with those with homogenates. From a comparison with the literature, snips glucuronidate the substrates employed at a rate much the same as in perfused preparations and some 30% less than the rate in isolated-hepatocyte suspensions; the advantages they offer in certain situations over these two techniques are discussed.

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Decreased UDP‐glucuronic acid in rat liver after ether narcosis

Cited by 30 publications

References 20 publications

Isoflurane as an anesthetic for experimental animal surgery

Isoflurane as an anesthetic for experimental animal surgery

Effect of Diethylether on the Formation of Paracetamol Sulphate and Glucuronide in Isolated Rat Hepatocytes

Liver snips. A simple, rapid and reproducible method for studying metabolism in small fragments of tissue, as applied to glucuronidation in rat liver

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