Decreased synovial fluid ghrelin levels are linked with disease severity in primary knee osteoarthritis patients and are increased following laser therapy

Zou, Yong; Deng, Hongyu; Mao, Zheng; Zhao, Chang; Huang, Ju; Liu, Gang

doi:10.1016/j.cca.2017.04.014

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…The synovial fluid ghrelin levels were increased following laser therapy. Ghrelin may play a protective role in knee OA (Zou et al, 2017). In this study, we observed that ghrelin mRNA and proteins expressions significantly decreased in both in vitro and in vivo OA models.…”

Section: Discussionsupporting

confidence: 51%

CircPan3 Promotes the Ghrelin System and Chondrocyte Autophagy by Sponging miR-667-5p During Rat Osteoarthritis Pathogenesis

Zeng

Zhang

Liao

et al. 2021

Front. Cell Dev. Biol.

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This study aimed to investigate the potential roles of circRNAs in regulating osteoarthritis (OA)-related ghrelin synthesis, autophagy induction, and the relevant molecular mechanisms. Results showed that Col2a1, Acan, ghrelin, and autophagy-related markers expression were downregulated, while matrix metalloproteinase 13 (MMP13) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) expressions increased in both IL-1β-induced rat chondrocytes and cartilage tissues of OA rats. A total of 130 circRNAs and 731 mRNAs were differentially expressed in IL-1β-induced rat chondrocytes. Among them, we found that circPan3 expression was significantly decreased in both cellular and animal OA models. CircPan3 directly targeted miR-667-5p. CircPan3 overexpression promoted Col2a1, Acan, ghrelin, beclin 1, and LC3-II expression but reduced MMP13 and ADAMTS5 expression in rat chondrocytes, whereas overexpression of miR-667-5p exhibited opposite effects on the above markers. Furthermore, we found that miR-667-5p bound directly to the 3′-UTR sequence of ghrelin gene. Moreover, the circPan3-induced alterations in chondrocytes were antagonized by miR-667-5p overexpression. Taken together, our findings demonstrate that circPan3 promotes ghrelin synthesis and chondrocyte autophagy via targeting miR-667-5p, protecting against OA injury. This study provided experimental evidence that circPan3/miR-667-5p/ghrelin axis might serve as targets of drug development for the treatment of OA.

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Section: Discussionsupporting

confidence: 51%

CircPan3 Promotes the Ghrelin System and Chondrocyte Autophagy by Sponging miR-667-5p During Rat Osteoarthritis Pathogenesis

Zeng

Zhang

Liao

et al. 2021

Front. Cell Dev. Biol.

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“…In addition, systemic and local delivery of ghrelin markedly ameliorated the OA severity in both surgically induced OA models, which implied a protective function of ghrelin in the degeneration of articular cartilage . In two of our previous cross‐sectional studies, we found that in both knee primary and traumatic OA, SF ghrelin levels were negative related to disease severity .…”

Section: Introductionmentioning

confidence: 50%

Attenuated levels of ghrelin in synovial fluid is related to the disease severity of ankle post‐traumatic osteoarthritis

Zou

Yang³

et al. 2019

BioFactors

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Post‐traumatic osteoarthritis (PTOA) of ankle joints results in pain and reduced joint function. Ghrelin, a 28‐amino‐acid polypeptide, has been previously identified as the first cognate natural ligand that binds to the growth hormone secretagogue receptor. In the present study, ghrelin has been validated to exert cartilage‐protective and anti‐inflammatory effects. The current study was aimed at investigating the potential role of the levels of serum and synovial fluid (SF) ghrelin on the severity of disease in patients suffering from ankle PTOA. Ninety‐seven patients with ankle osteoarthritis who received an arthroscopical examination and debridement or replacement of the ankle joint were included in the study cohort. Meanwhile, 95 healthy individuals (whose age and sex were matched) who received periodic body checkups were enrolled as healthy controls. Enzyme‐linked immunosorbent assay (ELISA) was used to analyze the ghrelin levels in serum and SF. SF was also probed for cartilage degradation enzyme matrix metalloproteinases‐3 (MMP‐3) and tumor necrosis factor alpha (TNF‐α), which is a known pro‐inflammatory cytokine. The clinical evaluation was carried out using the American Orthopedic Foot and Ankle Society (AOFAS) ankle‐hindfoot rating scale and visual analogue scale (VAS). The radiographic severity was evaluated using the modified Kellgren–Lawrence (K–L) grading system. We scored for the modified Mankin's score to depict histopathological changes due to cartilage lesions. The diagnostic relevance of the ghrelin concentrations in the prediction of the radiographic grading (in comparison with MMP‐3 and TNF‐α) was evaluated by calculating the area under the curve of the receiver operating characteristic (ROC) curve. The serum abundance of ghrelin was not significantly altered between ankle PTOA patients and healthy controls. SF ghrelin was negatively correlated with radiographic progression determined by modified ankle K–L grades. In addition SF ghrelin concentrations were negatively related to VAS scores, and positively associated with AOFAS ankle‐hindfoot rating. Moreover, SF ghrelin was inversely proportional to the expressions of MMP‐3 and TNF‐α. ROC analysis curve demonstrated that ghrelin serves as a favorable marker for the diagnosis of radiographic severity by modified ankle K‐L grade. The ghrelin concentration in SF is negatively proportional to disease progression in patients suffering from ankle PTOA. Local administration of ghrelin may function as a decent adjuvant therapy to delay the progress of ankle PTOA. © 2019 BioFactors, 45(3):463–470, 2019

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“…Ghrelin has been proven to inhibit inflammation and promote cartilage growth. SF levels of ghrelin were negatively related to TNF-a, IL-6 levels, and disease severity in patients with primary KOA 28 . Another study showed that ghrelin were significantly associated with increased knee symptom, infrapatellar fat pad (IPFP) (local adipose tissue situated in the knee under the patella) signal intensity alteration 29 .…”

Section: Association With Clinical Scores and Changesmentioning

confidence: 93%

Osteoarthritis year in review 2018: biomarkers (biochemical markers)

Hosnijeh

Bierma-Zeinstra

Bay‐Jensen

2019

Osteoarthritis and Cartilage

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The aim of this narrative review is to summarize important findings from biochemical marker studies relevant to osteoarthritis (OA) in the context of new discoveries and clinical and scientific need. Design: We conducted a systematic search of electronic medical databases (Embase, Medline, Web of Science, Cochrane central) between 01-03-2017 and 31-03-2018. The search was restricted to human studies, English language and full text available publications while reviews were excluded. Only papers describing protein based biomarkers measured in human body fluids (blood, urine and synovial fluid (SF)) were included. Of the 992 papers, 86 were reviewed here, with inclusion primarily based on relevance to OA biochemical markers. Results: This review highlights a selection of studies based on their quality and perceived importance to the field mainly including those that 1 evaluate prognostic value of biomarkers for OA progression (i.e., biomarkers reflecting change in composition of joint tissues and biomarkers of inflammation) 2 , help in assessment of intervention efficacy, and 3 are innovative and uncover new candidate biomarkers, or use new approaches in biomarker discovery. Conclusions: Key findings and implications for possible clinical utility of biochemical markers are summarized and discussed. Given the paucity of robust biomarkers within the field, and the heterogeneity of the condition, enormous works are needed for development and validation of novel and clinically applicable biomarkers to reduce the impact of this highly prevalent and debilitating condition.

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Decreased synovial fluid ghrelin levels are linked with disease severity in primary knee osteoarthritis patients and are increased following laser therapy

Cited by 13 publications

References 32 publications

CircPan3 Promotes the Ghrelin System and Chondrocyte Autophagy by Sponging miR-667-5p During Rat Osteoarthritis Pathogenesis

CircPan3 Promotes the Ghrelin System and Chondrocyte Autophagy by Sponging miR-667-5p During Rat Osteoarthritis Pathogenesis

Attenuated levels of ghrelin in synovial fluid is related to the disease severity of ankle post‐traumatic osteoarthritis

Osteoarthritis year in review 2018: biomarkers (biochemical markers)

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