Decreased sensitivity of transformed 3T3-SV40 cells treated with N-acetylcysteine to bacterial invasion

Ia, Gamaleĭ; Tn, Efremova; Km, Kirpichnikova; Yy, Komissarchik; Lv, Kever; Yv, Polozov; Sy, Khaitlina

doi:10.1007/s10517-006-0300-3

Cited by 7 publications

(6 citation statements)

References 14 publications

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“…The decrease in GSH may therefore be involved in ROS elevation in these cell lines. Previous studies have shown that NAC reduces oxidative stress by increasing GSH level and GSH/GSSG ratio and decreasing ROS and lip-id peroxidation (Amer et al, 2008;Gamalei et al, 2006). In this present study, NAC caused significant decreases in ROS levels in HepG2 and HEK 293 cells treated with Cd without significant increase in GSH levels.…”

Section: Discussionsupporting

confidence: 63%

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

Lawal

Ellis

2010

J. Toxicol. Sci.

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-Cadmium (Cd) is a toxic heavy metal with no uniform mechanism of toxicity. In this the present study, the toxic effect of 5, 10 and 50 μM of Cd chloride was compared in three human cell lines; a human hepatoma cell line HepG2, a human astrocytoma cell line 1321N1, and a human embryonic kidney cell HEK 293. The results indicate a decrease in the viability of all three cell lines following exposure to Cd with HepG2 cells (IC 50 = 13.96 μM) showing the most sensitivity when measured using the MTT assay. There was significant increase in lactate dehydrogenase leakage, DNA damage, malondialdeyde and antioxidant enzymes activities in all three cell lines especially at 50 μM Cd. Significant decreases in ATP production were also observed at all Cd concentrations in HepG2 and HEK 293 cell lines. ROS levels significantly increase and GSH/GSSG ratio significantly decrease in all the three cell lines after Cd exposure, but these effects were attenuated by the presence of N-acetylcysteine (NAC). The present study therefore shows that ROS production and glutathione (GSH) depletion may play a role in Cd-induced toxicity in all the three cell lines. The endogenous levels of protective enzymes as well as their responsiveness are likely to determine a cell's susceptibility to Cd.

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Section: Discussionsupporting

confidence: 63%

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

Lawal

Ellis

2010

J. Toxicol. Sci.

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“…These factors were employed to find out whether bacterial invasion is sensitive to the cell type and the presence of antioxidant N-acethylcysteine (NAC) in the culture medium. The results of these experiments confirmed different effects of NAC on 3T3, 3T3-SV40 and HeLa cells [78]. Incubation of HeLa cells with NAC increased penetration of grimelysin-producing bacteria by 1.5-2 times for wild-type Serratia grimesii and by 3-3.5 times for recombinant Escherichia coli expressing the grimelysin gene [79].…”

Section: Cellular Factors Involved In Serratia Invasionsupporting

confidence: 63%

Bacterial Actin-Specific Endoproteases Grimelysin and Protealysin as Virulence Factors Contributing to the Invasive Activities of Serratia

Khaitlina

Bozhokina

Tsaplina

et al. 2020

IJMS

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The article reviews the discovery, properties and functional activities of new bacterial enzymes, proteases grimelysin (ECP 32) of Serratia grimesii and protealysin of Serratia proteamaculans, characterized by both a highly specific “actinase” activity and their ability to stimulate bacterial invasion. Grimelysin cleaves the only polypeptide bond Gly42-Val43 in actin. This bond is not cleaved by any other proteases and leads to a reversible loss of actin polymerization. Similar properties were characteristic for another bacterial protease, protealysin. These properties made grimelysin and protealysin a unique tool to study the functional properties of actin. Furthermore, bacteria Serratia grimesii and Serratia proteamaculans, producing grimelysin and protealysin, invade eukaryotic cells, and the recombinant Escherichia coli expressing the grimelysin or protealysins gene become invasive. Participation of the cellular c-Src and RhoA/ROCK signaling pathways in the invasion of eukaryotic cells by S. grimesii was shown, and involvement of E-cadherin in the invasion has been suggested. Moreover, membrane vesicles produced by S. grimesii were found to contain grimelysin, penetrate into eukaryotic cells and increase the invasion of bacteria into eukaryotic cells. These data indicate that the protease is a virulence factor, and actin can be a target for the protease upon its translocation into the host cell.

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“…At the same time, reactive oxygen metabolites play a major role in the regulation of proliferative processes [3]. For evaluation of the role of reactive oxygen metabolites in the morphogenetic effect of sedatin on the neonatal myocardium, we studied the intensity of free radical oxidation in the myocardium of newborn animals receiving 5 injections of sedatin by the method of CL.…”

Section: Resultsmentioning

confidence: 99%

Effect of synthetic dermorphin analogues on tissue homeostasis in the myocardium of newborn albino rats

et al. 2007

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DNA synthesis and state of the nucleolar apparatus in myocardial cells of newborn albino rats receiving intraperitoneal injection of sedatin, a synthetic dermorphin analogue, were studied by means of autoradiography and silver impregnation. Labeling intensity significantly increased, while the number of nucleoli in nuclei decreased. Chemiluminescence study showed that the concentration of reactive oxygen metabolites significantly decreased in the myocardium of treated animals after sedatin administration. Non-arginine analogue of sedatin had little effect on tissue homeostasis in the myocardium.

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Decreased sensitivity of transformed 3T3-SV40 cells treated with N-acetylcysteine to bacterial invasion

Cited by 7 publications

References 14 publications

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

Bacterial Actin-Specific Endoproteases Grimelysin and Protealysin as Virulence Factors Contributing to the Invasive Activities of Serratia

Effect of synthetic dermorphin analogues on tissue homeostasis in the myocardium of newborn albino rats

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