Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

Kobayashi, Takahiko; Ishida, Junich; Shimizu, Yuichi; Kawakami, Hiroshi; Suda, Goki; Muranaka, Toshiya; Asaka, Masahiro; Sakamoto, Naoya

doi:10.1177/1010428317694547

Cited by 8 publications

(5 citation statements)

References 42 publications

(78 reference statements)

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“…Many other RBPs also exhibit changed expression in GC tissues. The hnRNP‐A1, ‐A2/B1, ‐C, ‐F, ‐K, polypyrimidine tract‐binding protein (PTBP)‐1, ‐3, poly r(C) binding protein 2, IMP‐3, Musashi‐1, and non‐POU domain containing octamer binding protein (NONO) were upregulated (C. Chen et al, ; Huang et al, ; Jing et al, ; P. T. Lee et al, ; D. Li et al, ; Park et al, ; Shou et al, ; Sugiyama et al, ; S. H. Xu et al, ; R. Yang et al, ; Zhou et al, ), while RNA‐binding motif (RBM)‐4, ‐5, RBM single‐stranded interacting protein 3 (RBMS3), and PCBP1 were inhibited (Ji et al, ; Kobayashi et al, ; Y. Wu et al, ; Yong et al, ; T. Zhang et al, ). These aberrant expression patterns were thought to associate with pathologic feature and survival of GC patients, but further investigations are needed to reveal their functions and direct targets during cancer development.…”

Section: Major Functions Of Rbps In Gi Cancermentioning

confidence: 99%

“…H. Xu et al, 2018;R. Yang et al, 2016;, while RNA-binding motif (RBM)-4, -5, RBM single-stranded interacting protein 3 (RBMS3), and PCBP1 were inhibited (Ji et al, 2017;Kobayashi et al, 2017;Yong et al, 2016;. These aberrant expression patterns were thought to associate with pathologic feature and survival of GC patients, but further investigations are needed to reveal their functions and direct targets during cancer development.…”

Section: Function Of Rbps In Gastric Cancermentioning

confidence: 99%

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Diverse roles of RNA‐binding proteins in cancer traits and their implications in gastrointestinal cancers

Masuda

Kuwano

2018

WIREs RNA

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Gene expression patterns in cancer cells are strongly influenced by posttranscriptional mechanisms. RNA-binding proteins (RBPs) play key roles in posttranscriptional gene regulation; they can interact with target mRNAs in a sequence-and structure-dependent manner, and determine cellular behavior by manipulating the processing of these mRNAs. Numerous RBPs are aberrantly deregulated in many human cancers and hence, affect the functioning of mRNAs that encode proteins, implicated in carcinogenesis. Here, we summarize the key roles of RBPs in posttranscriptional gene regulation, describe RBPs disrupted in cancer, and lastly focus on RBPs that are responsible for implementing cancer traits in the digestive tract. These evidences may reveal a potential link between changes in expression/function of RBPs and malignant transformation, and a framework for new insights and potential therapeutic applications.

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Section: Major Functions Of Rbps In Gi Cancermentioning

confidence: 99%

Section: Function Of Rbps In Gastric Cancermentioning

confidence: 99%

Diverse roles of RNA‐binding proteins in cancer traits and their implications in gastrointestinal cancers

Masuda

Kuwano

2018

WIREs RNA

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“…RBM5 is a ubiquitously expressed RBP known to regulate mRNA and protein levels, as well as alternative splicing of genes involved in regulation of apoptosis, cell cycle, cytoskeleton, and drug metabolism 43 , 69 , 73 , 85 – 87 . RBM5 can both stimulate and suppress splicing at specific splice sites via different mechanisms 41 , 42 , 67 , 69 , 72 , 88 .…”

Section: Discussionmentioning

confidence: 99%

Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington’s disease

et al. 2023

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RNA-binding proteins (RBPs) are key players regulating RNA processing and are associated with disorders ranging from cancer to neurodegeneration. Here, we present a proteomics workflow for large-scale identification of RBPs and their RNA-binding regions in the mammalian brain identifying 526 RBPs. Analysing brain tissue from males of the Huntington’s disease (HD) R6/2 mouse model uncovered differential RNA-binding of the alternative splicing regulator RBM5. Combining several omics workflows, we show that RBM5 binds differentially to transcripts enriched in pathways of neurodegeneration in R6/2 brain tissue. We further find these transcripts to undergo changes in splicing and demonstrate that RBM5 directly regulates these changes in human neurons derived from embryonic stem cells. Finally, we reveal that RBM5 interacts differently with several known huntingtin interactors and components of huntingtin aggregates. Collectively, we demonstrate the applicability of our method for capturing RNA interactor dynamics in the contexts of tissue and disease.

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“…RBPs also play a vital role in the initiation of GC. It was reported that RBM5 downregulation was involved in GC progression, behaving as a tumor suppressor gene in GC ( 19 ). RBPs have the potential to be used as novel biomarkers as well.…”

Section: Discussionmentioning

confidence: 99%

RNA-Binding Protein COL14A1, TNS1, NUSAP1 and YWHAE Are Valid Biomarkers to Predict Peritoneal Metastasis in Gastric Cancer

et al. 2022

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Gastric cancer (GC) is the third leading cause of tumor related mortality worldwide. Peritoneal metastasis (PM) occurs in more than half of advanced GC patients, leading to poor prognosis. Therefore, the GSE62254 cohort was used to construct a signature consisting of four RNA-binding proteins (RBP) to predict the possibility of PM in GC patients. Then, ROC curves were plotted followed by calculation of AUCs, showing that the signature had a similar predictive accuracy compared with the TNM staging system. Importantly, the capability of prediction was enhanced by combining the classifier and TNM staging. In order to validate the expression of the four RBPs in GC tissues with and without PM, immunohistochemistry was further performed on samples from 108 patients. The differential expression of COL14A1, TNS1, NUSAP1 and YWHAE was in accordance with the emergence of PM. Afterwards, we produced Kaplan–Meier curves according to the signature and differential expression of the RBPs in patients. Finally, CCK-8 assays were performed to verify the effect on cell proliferation, finding that COL14A1 and TNS1 promoted cell proliferation, while NUSAP1 and YWHAE led to suppressed cell proliferation. In conclusion, the four-RBP-based signature, combined with TNM staging, has the potential to predict risk of PM in GC.

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Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

Cited by 8 publications

References 42 publications

Diverse roles of RNA‐binding proteins in cancer traits and their implications in gastrointestinal cancers

Diverse roles of RNA‐binding proteins in cancer traits and their implications in gastrointestinal cancers

Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington’s disease

RNA-Binding Protein COL14A1, TNS1, NUSAP1 and YWHAE Are Valid Biomarkers to Predict Peritoneal Metastasis in Gastric Cancer

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