Decreased Ratios of Interleukin-1&amp;lt;i&amp;gt;β&amp;lt;/i&amp;gt; and Intercellular Adhesion Molecule 1 in Gingival Crevicular Fluid after Scaling and Root Planing Associated with Periodontal Pocket Healing

Hieda, Yuriko; Usui, Michihiko; Nakamura, Taiji; Morishita, Masaki; Hanatani, Tomoya; Moritani, Yuki; Koga, Yukiko; Kasai, Shingo; Inoue, Maki; Nakashima, Keisuke

doi:10.4236/ojst.2017.79031

Cited by 4 publications

(4 citation statements)

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“…Although bleeding on probing (BOP) is a classical and beneficial indicator of periodontitis progression [ 19 , 20 ], it cannot distinguish between minute and massive bleeding and is not quantitative. Recently, inflammatory cytokines in gingival crevicular fluid (GCF), such as MMP-8, IL-1, and TNF-α, were measured and found to be associated with disease progression and response to treatment [ 21 , 22 , 23 ]. However, the measurement of these cytokines is performed by ELISA, which is expensive in terms of purchasing equipment and testing costs.…”

Section: Discussionmentioning

confidence: 99%

The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species

et al. 2022

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The trypsin-like peptidase activity assay kit measures the trypsin-like protease produced by three red-complex species, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, causing periodontitis, and detects the presence of these bacteria in samples. The purpose of this study was to investigate the relationship between the detection of TLPs by a novel TLP-AA, ADCHECK and the detection of red-complex pathogens by real-time PCR using tongue swabs from patients with periodontitis. The detection limit of trypsin-like protease activity by ADCHECK was validated using the culture supernatants of two different Porphyromonas gingivalis bacterial strains. Real-time PCR was performed to determine the number of red-complex species in the tongue coatings of patients with periodontal disease. Trypsin-like protease activity in tongue-swab samples was scored using ADCHECK. ADCHECK successfully detected trypsin-like protease activity in 103 Porphyromonas gingivalis bacterial strains. The specificity, positive predictive value, negative predictive value, and accuracy of ADCHECK for the presence of red-complex pathogens determined by real-time PCR were 90%, 97%, 98%, and 92%, respectively. ADCHECK is an effective tool for the detection of red-complex pathogens.

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Section: Discussionmentioning

confidence: 99%

The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species

et al. 2022

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“…La relación entre los valores de los parámetros clínicos con los niveles de IL-1α e IL-1β, mostró una correlación significativa, que no se observó con el TNF-α, estos resultados son similares a los obtenidos por otras investigaciones que reportan una mayor concentración de mediadores en tejido inflamado, y en relación directa con los parámetros periodontales [30][31][32][33] . Por el contrario, otros estudios no han mostrado correlaciones entre los parámetros clínicos periodontales y los mediadores de la inflamación estudiados 21,26,34 .…”

Section: Discussionunclassified

Evaluación de los mediadores IL-1α, IL-1β y TNF-α, en fluido gingival crevicular de pacientes con periodontitis

Simone

Escalona

Ávila

et al. 2019

Odontol. sanmarquina

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Objetivo. Evaluar los niveles de mediadores químicos inflamatorios como la interleucina- 1α (IL-1α), interleucina-1β (IL-1β), factor de necrosis tumoral-α (TNF-α) y los parámetros clínicos en pacientes diagnosticados con periodontitis crónica o agresiva, antes y después de la terapia periodontal no quirúrgica (TPNQ). Métodos. Se tomaron muestras de fluido gingival crevicular (FGC) en 11 pacientes diagnosticados con periodontitis y 11 controles sanos para evaluar niveles de IL-1α, IL-1β, TNF-α antes y después de la TPNQ, mediante la técnica de ELISA (Enzyme-Linked Immuno Sorbent Assay). Resultados. En el grupo con periodontitis se observó disminución significativa de los parámetros clínicos después del tratamiento (p<0,05). En FGC, IL-1β presentó los niveles más elevados, seguido de IL-1α y TNF-α. Posterior a la TPNQ, IL-1β disminuyó 17 veces su concentración e IL-1α 4 veces en relación a los valores iniciales, sin embargo, solo se observó una relación estadísticamente significativa para TNF-α. Las mayores correlaciones con los parámetros clínicos de la enfermedad periodontal se observaron para IL-1β (p<0,01), seguido por IL-1α (p<0,05) y una baja correlación con TNF-α. Conclusiones. En este trabajo, el grupo de pacientes con periodontitis presentó mayores niveles de IL-1α, IL-1β y TNF-α en FGC, siendo IL-1β la citocina más elevada. Posterior al TPNQ, se observó disminución significativa de los valores clínicos periodontales. Así mismo, las interleucinas redujeron sus valores promedios, sin alcanzar los del grupo control. Las correlaciones observadas entre los índices periodontales y los mediadores sugieren mayor correlación de IL-1β con los signos clínicos de la enfermedad periodontal.

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“…The present authors previously used a membrane antibody array to measure levels of multiple cytokines in GCF from patients with chronic periodontitis before and after scaling and root planing (SRP). The findings suggested that IL-1β in GCF is related to periodontal healing [14].…”

Section: Introductionmentioning

confidence: 89%

Associations of cytokine levels in gingival crevicular fluid of mobile teeth with clinical improvement after initial periodontal treatment

et al. 2020

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Studies suggest that analysis of gingival crevicular fluid (GCF) is useful for evaluating periodontal status. In this study, clinical variables related to tooth mobility, and multiple cytokine levels in proximate GCF, were measured at four time points during initial periodontal treatment: before treatment (baseline), after supragingival scaling, after occlusal adjustment, and after scaling and root planing (SRP); 20 teeth from 13 patients with periodontitis were included. Baseline interleukin (IL)-10 level in GCF was significantly higher around teeth that showed substantial improvement in periodontal epithelial surface area (PESA) after SRP than around teeth without PESA improvement. IL-3 and IL-16 levels in GCF at baseline were significantly higher around teeth with a periodontal inflamed surface area (PISA) of 0 mm 2 after SRP than around teeth without PISA improvement. In addition, baseline IL-7, IL-11, and IL-12p40 levels in GCF were significantly lower around teeth with decreased mobility after occlusal adjustment than around teeth without decreased mobility. These results suggest that pre-treatment cytokine levels in GCF are useful in predicting the effects of initial periodontal treatment.

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Decreased Ratios of Interleukin-1<i>β</i> and Intercellular Adhesion Molecule 1 in Gingival Crevicular Fluid after Scaling and Root Planing Associated with Periodontal Pocket Healing

Cited by 4 publications

References 34 publications

The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species

The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species

Evaluación de los mediadores IL-1α, IL-1β y TNF-α, en fluido gingival crevicular de pacientes con periodontitis

Associations of cytokine levels in gingival crevicular fluid of mobile teeth with clinical improvement after initial periodontal treatment

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