Decreased Osteogenic Differentiation of Mesenchymal Stem Cells in Alcohol-induced Osteonecrosis

Suh, Kuen Tak; Kim, Seung Wook; Roh, Hyoung Lok; Youn, Myung Soo; Jung, Jin Sup

doi:10.1097/01.blo.0000150568.16133.3c

Cited by 126 publications

(75 citation statements)

References 24 publications

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“…However, few studies are associated with treatment of alcohol-induced ON. Consistent with previous observations [10,20,24,28,46,50], our data suggest alcohol can induce adipogenesis both in vitro and in vivo, a change found in early stage ON. Adipogenic differentiation is a complex process regulated by many factors.…”

Section: Discussionsupporting

confidence: 93%

Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Wang

Yin

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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Alcohol can induce adipogenesis by bone marrow stromal cells and may cause osteonecrosis of the femoral head. Currently, there are no medications available to prevent alcohol-induced osteonecrosis. We hypothesized puerarin, a Chinese herbal medicine with antioxidative and antithrombotic effects, can prevent alcohol-induced adipogenesis and osteonecrosis. Both bone marrow stromal cells (in vitro) and mice (in vivo) were treated either with ethanol or with ethanol and puerarin, with an untreated group serving as a control. In the in vitro study, the number of adipocytes, contents of triglycerides, and levels of PPARc mRNA expression were decreased and alkaline phosphatase activity, contents of osteocalcin, and levels of osteocalcin mRNA expression were increased in cells treated with both alcohol and puerarin, compared with cells treated with alcohol only. In the in vivo study, marrow necrosis, fat cell hypertrophy and proliferation, thinner and sparse trabeculae, diminished hematopoiesis, and increased empty osteocyte lacunae in the subchondral region of the femoral head were observed in mice treated with alcohol. However, no such changes were seen in femoral heads of mice treated with alcohol and puerarin. The data suggest puerarin can inhibit adipogenic differentiation by bone marrow stromal cells both in vitro and in vivo and prevents alcohol-induced osteonecrosis in this model.

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Section: Discussionsupporting

confidence: 93%

Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Wang

Yin

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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“…However, it is difficult to obtain bone marrow specimens from patients with earlier-stage ONFH because of the absence of subjective symptoms and ethical concerns. Moreover, several researchers have reported reduced BMSC osteogenic differentiation in ONFH patients requiring hip replacement for late-stage disease [9,20]. In our study, BMSCs activities were not found to be significantly different in alcohol-induced ONFH patients who had stopped drinking and those that continued to consume alcohol (data not shown).…”

Section: Discussioncontrasting

confidence: 57%

“…Reports published recently suggest that BMSC numbers and osteogenic differentiation ability are depressed in patients with ONFH, which, in turn, suggests that ONFH is a disease of bone cells and/or BMSCs [5,6,9,20]. In addition, several reports have indicated that BMSC isolation yield and osteogenic capacity are related to the risk factors of ONFH [6,9].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis

Yoo

Song²,

Koo

et al. 2008

International Orthopaedics (SICOT)

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We investigated whether the proliferation activities and osteogenic capacities of bone marrow stromal cells (BMSCs) are depressed in patients with osteonecrosis of the femoral head (ONFH). BMSCs were isolated from fresh bone marrow of the iliac crests of 54 donors and were differentiated into osteogenic lineage in vitro. The results of 27 consecutive patients with ONFH (16 idiopathic and 11 alcohol-induced) were compared with those of 27 patients with a nonnecrotic hip disorder. The proliferative activities of BMSCs in patients with ONFH were not found to be reduced and their osteogenic capacities (alkaline phosphatase activity and calcium deposition amount) were unaltered during in vitro differentiation. Results from patients with idiopathic or alcohol-induced ONFH were similar to those of matched patients with nonnecrotic disorder. These findings suggest that the osteogenic potentials of BMSCs are not defective in patients with ONFH.

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“…Likewise, our results showed that in the setting of osteonecrosis, aMSCs showed superior osteogenic potential compared with bmMSCs as measured by alkaline phosphatase activity. This may be explained partly in previous studies showing that patients with corticosteroidinduced and alcohol-induced osteonecrosis have decreased osteogenesis from their bmMSCs [7,9,12,17,34]. Greater physiologic stress in the bone marrow may contribute to the discrepant osteogenesis profile of MSCs based on native tissue source in patients with these insults.…”

Section: Discussionmentioning

confidence: 99%

“…Reported predictors of failure include size of the necrotic lesion, etiology of the osteonecrosis, and proliferation potential of the bmMSCs based on fibroblast colony-forming units [13,22]. In addition, previous studies have shown that patients with corticosteroid-induced and alcohol-induced osteonecrosis have decreased osteogenesis from their bmMSCs [7,9,12,17,34] and could account for the limited results obtained in certain osteonecrosis groups. Identifying other potential sources of MSCs is of critical importance for management of patients who may be considered at high risk for failure.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived Mesenchymal Stem Cells Are Phenotypically Superior for Regeneration in the Setting of Osteonecrosis of the Femoral Head

Wyles

Houdek

Crespo‐Diaz

et al. 2015

Clin Orthop Relat Res

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Background Bone marrow-derived mesenchymal stem cells (bmMSCs) have been used as a cellular therapeutic option for treatment of osteonecrosis of the femoral head. However, use of bmMSCs as a treatment adjuvant for orthopaedic disorders in general has achieved limited success. Adipose-derived MSCs (aMSCs) may be a moreefficient regenerative cell source given their greater quantity and protection from physiologic stress.

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Decreased Osteogenic Differentiation of Mesenchymal Stem Cells in Alcohol-induced Osteonecrosis

Cited by 126 publications

References 24 publications

Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis

Adipose-derived Mesenchymal Stem Cells Are Phenotypically Superior for Regeneration in the Setting of Osteonecrosis of the Femoral Head

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